3D打印技术制备生物医用高分子材料的研究进展

620136ACTAPOLYMERICASINICANo．6Jun．2013722*802012-12-272013-01-215100310321174142511731842107412951021003512330042011DFR51090．＊＊[e-mail]10.3724/SP．J．1105.2013.12430··3D*＊＊1300223D．．3D．3D3D．3D．3D3D3D2080CADCT3D3D1～3．3D“、”．CT3DSTL3D．3DSLA、FDM、SLS3DP13．Fig．1Schematicillustrationsoftypical3DprintingtechnologiesastereolithographyReprintedfromRef.4Copyright2010withpermissionfromElsevierandbfuseddepositionmodelingReprintedfromRef．5Copyright2002withpermissionfromElsevier63D3D、、6．3D、、6～8．、、．3DCT、MRI．3D．3D．3D、．Fig．2ASEMimagesofPPFscaffoldswithachexagonandbdsquareporesfabricatedbySLAReprintedwithpermissionfromRef．11Copyright2007AmericanChemicalSocietyandBSEMimagesofporousPLA-FA3gyroidstructuresbuiltbySLAReprintedwithpermissionfromRef．13Copyright2009AmericanChemicalSociety11.1SLA．、1a．SLA、、．SLA3D．SLAPPF、DL-PLA、ε-PCL、、．DEFN-NVP910．3D、、11．ChoPPFSLA327201312．PPFPPF12．2、8．Table1Examplesofscaffoldsfabricatedby3DprintingMaterial3DprintingtechnologyApplicationRef．OCM-2/HASLAMandibularscaffold28HyaluronicacidderivativeSLAAuriclescaffold39PEGT/PBT3DfiberdepositionFemoralandtibialscaffolds50PCLSLSMandibularcondylescaffold57PLGA95/5/HASLSModelofahumanfourthmiddlephalanx64Ca-PSLSProximalfemoralcondylescaffold65DL-PLAε-PCL．3D．Grijpma3DL-PLA-FA3N-NVP2B13．NVP．．NVPNVP．．PLA10．PLAPLA600g/mol．ε-PCLPCL14．CAD．465μm70.5%．PLADL--ε-PLACL、、15．3DCAD95%．．．MatsudaPTMC、、16．PEG．PEG、．17～19．、、．20．、21．3D/．RGD、、22～24．25．．．．HAHA．42763DChoPPF/HA3D26．HA/．HA．MatsuoL-/HAPLLA/HA27．-OCM-2/HA2829．CO2．1.23031．．．3D．PEGRGD、32．33．ArcautePEGPEG-DA34．/．PEG．ButcherPEG-DA/3a35．5.3～74.6kPa．．21100%．PLA-PEG-PLA、36．．Fig．3aPrintedheterogeneousvalvewhererootwasformedwith700MWPEG-DAhydrogelwhiletheleafletswereformedwith700/8000MWPEG-DAhydrogels．KeyfeaturessuchasthecoronaryostiumandsinuseswerepresentedReproducedwithpermissionfromRef．35Copyright2012IOPPublishing．bPhotographof3Dprintedhyaluronicacid/dextranhydrogelScalebarindicates25mmReprintedwithpermissionfromRef．37Copyright2011AmericanChemicalSociety．38．．HUVEC4．HEMA/Dex-HEMA/HAc3D3739．39．5272013Fig．4ImmunofluorescenceimagesshowingthebiologicalfunctionalityoftheHUVECseededscaffoldsThepresenceofendothelialcellspecificmarkersCD31aandvonWillebrandfactorvWFbshowedthattheconfluentendothelialcellsretainedtheirphenotypeduringthecultureperiod．MoreoverKi67labelingcshowedHUVECadheredonthescaffoldsandexpressingthismarkerarrowsdindicatingaproliferativestateafter4daysincultureReprintedfromRef．38Copyright2012withpermissionfromElsevier2FDM、、1b．FDMABS、、、、、．、、、．FDM．、、40．HutmaterPCLFDM、3D541．160～700μm48%～77%．4～77MPa0.4～3.6MPa4%～28%．．3～4．3D42．TeohPCLFDM43．2．3DPCL．HenninkBMGε-CLHMGε-CLPHMGCL3D54445．．PHMGClHMG∶Cl=8∶923DBalb/cPHMGCl360%PHMGClPCL．62763DFig．5Macroscopicimagesof3D-printedscaffoldsbeforeimplantationtopviewofaPCLandbPHMGCLscaffoldsandsideviewofcPCLanddPHMGCLscaffoldsScalebaris2.5mmReprintedfromRef．45Copyright2012withpermissionfromElsevier、．PHMGClPCL．ChoPCL/PLGA3D46．600μm69.6%0.812.9MPa．3D3D47．RGDPCL/PLGA、48．L--ε-PLLACL3D49．FDM．Woodfield-/PEGT/PBT6CT3D50．6．3D．20%TCPhMSCs51．hMSCsPCL-TCPhMSCs350%．PCL-TCP15%PT152h52．PT15．PT15．PLGA/TCP3D1253．．PCL/PLGA/TCP3D123D54．3SLS、．7272013．SLASLS、．SLS、、．．SLS3D．、、、5556．DasPCLSLS3D52～67MPa2.0～3.2MPa57．3D．PCL．NaCl3D58．80wt%NaCl90%3D1mm．99.99%．59．．C2C12345kPaPCL2111C2C12．SLSPCL60．PCL．SLSPCL、PLASLS61．．HAHASLS/HA3D6263．WiriaSLS125～250μmPLGA95/5HAHA/TCP64．Wang-PHBV/Ca-P3D65．/-2rhBMP-2．PEEK、、、-666～68HA69～71SLS．43D3D3DPCAD．3DP、、、．ThericsSherwood3DPPLGA/PLLAPLGA/TCP-72．90%55%．6．．．53D、、．82763D．3D．、3D73．3D123．PEGPEG-DAPEGPEG-DMA/3D7475．7576．RGD76．D’LimaPEG-DMA/77．PEG．26%．．．3D．CaCl2Ca2+7879．hCMPCs3D1792%89%hCMPCs3D．．80．/．．．pHpH3D．Yoo81．．63D3D3D．3D．3D、．、．、、．3D、、．．3D．3D．3D．3D、、、．3D9272013．3D、．REFERENCES1LeongKFCheahCMChuaCK．Biomaterials2003242363～23782YeongWYChuaCKLeongKF．ChanfrasekaranTrendsinBiotechnology200422643～6523SeolYJJangTYChoDW．SoftMatter201281730～17354MelchelsFPWFeijenJGrijpmaDW．Biomaterials2010316121～61305ZeinIHutmacherDWTanKCTeohSH．Biomaterials2002231169～11856DerbyB．Science2012338921～9267MinnsRJBibbRBanksRSuttonRA．MedicalEngineering＆Physics200325523～5268MahaisavariyaBSitthseripratipKOrisTongdeeT．InjuryExtra200637176～1809CookeMNFisherJPDeanDRimnacCMikosAG．JBiomedMaterResBApplBiomater200264B65～6910MelchelsFPWFeijenJGrijipmaDW．Biomaterials2009303801～380911LeeKWWangSFoxBCRitmanELYaszemskiMJLuL．Biomacromolecules200781077～108412ShinJHLeeJWJungJHChoDWLimG．JMaterSci2011465282～528713JansenJMelchelFPWGrijpmaDWFeijenJ．Biomacromolecules200910214～22014ElomaaLTeieiraSHakalaRKorhonenHGrijpmaDWSeppalaJU．ActaBiomaterialia201173850～385615MelchelsFPWBertoldiKGabbrielliRVeldersAHFeijenJGrijpmaDW．Biomaterials2010316909～691616KwonIKMatsudaT．Biomaterials2005261675～168417LeeSJKangHWParkJKRhieJWHahnSKChoDW．BiomedMicrodevices200810233～24118MizutaniMArnoldSCMatsudaT．Biomacromolecules20023668～67519MatsudaTMizutaniM．JBiomedMaterRes200262395～40320MelchelsFPWTonnarelliBOlivaresALMartinILacroixDFeijenJWendtDJGrijpmaDW．Biomaterials2011322878～288421KimKDeanJBreithauptRMikosAGFisherJP．Biomaterials2011323750～376322LanPXLeeJW