BD-FACSVerse-流式仪器使用指导

bdbiosciences.com23-11463-00Rev.016/2011ForResearchUseOnlyBecton,DickinsonandCompanyBDBiosciencesSanJose,[e-mail]@europe.bd.comBDFACSVerse™SystemUser’sGuideCopyrights©2011,Becton,DickinsonandCompany.Allrightsreserved.Nopartofthispublicationmaybereproduced,transmitted,transcribed,storedinretrievalsystems,ortranslatedintoanylanguageorcomputerlanguage,inanyformorbyanymeans:electronic,mechanical,magnetic,optical,chemical,manual,orotherwise,withoutpriorwrittenpermissionfromBDBiosciences.Theinformationinthisguideissubjecttochangewithoutnotice.BDBiosciencesreservestherighttochangeitsproductsandservicesatanytimetoincorporatethelatesttechnologicaldevelopments.Althoughthisguidehasbeenpreparedwitheveryprecautiontoensureaccuracy,BDBiosciencesassumesnoliabilityforanyerrorsoromissions,norforanydamagesresultingfromtheapplicationoruseofthisinformation.BDBioscienceswelcomescustomerinputoncorrectionsandsuggestionsforimprovement.TrademarksCONTRAD®isaregisteredtrademarkofDeconLabs,Inc.FCAPArrayisatrademarkofSoftFlow,Hungary,Ltd.MicrosoftandWindowsareregisteredtrademarksofMicrosoftCorporation.BD,BDLogo,andallothertrademarksarepropertyofBecton,DickinsonandCompany.©2011BDPatentsTheBDFACSVerse™flowcytometeriscoveredbyoneormoreofthefollowingUSpatents:7,787,197;7,129,505;6,897,954;6,809,804;6,683,314;and6,510,007.RegulatoryinformationForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.Class1LaserProduct.FCCinformationWARNING:Changesormodificationstothisunitnotexpresslyapprovedbythepartyresponsibleforcompliancecouldvoidtheuser’sauthoritytooperatetheequipment.NOTICE:ThisequipmenthasbeentestedandfoundtocomplywiththelimitsforaClassAdigitaldevice,pursuanttoPart15oftheFCCRules.Theselimitsaredesignedtoprovidereasonableprotectionagainstharmfulinterferencewhentheequipmentisoperatedinacommercialenvironment.Thisequipmentgenerates,uses,andcanradiateradiofrequencyenergyand,ifnotinstalledandusedinaccordancewiththeinstructionmanual,maycauseharmfulinterferencetoradiocommunications.Operationofthisequipmentinaresidentialareaislikelytocauseharmfulinterferenceinwhichcasetheuserwillberequiredtocorrecttheinterferenceathisorherownexpense.ShieldedcablesmustbeusedwiththisunittoensurecompliancewiththeClassAFCClimits.ThisClassAdigitalapparatusmeetsallrequirementsoftheCanadianInterference-CausingEquipmentRegulations.CetappareilnumériquedelaclasseArespectetouteslesexigencesduRéglementsurlematérielbrouilleurduCanada.HistoryRevisionDateChangemade23-11463-00Rev.016/2011NewdocumentContentsChapter1:Introduction9Aboutthisguide.................................................10Safetysymbols..................................................12Systemtechnicalsupport...........................................13Chapter2:Aboutthesystem15Systemoverview.................................................16Cytometeroverview..............................................19Opticalcomponents..............................................22Fluidicscomponents..............................................24Systemoptionsandupgrades.......................................26BDFACSuitesoftwareoverview.....................................28BDFACSuitesoftwarecomponents..................................31Dailyworkflow..................................................36Chapter3:Systemsetup37Settingupthesystem.............................................38Managinguseraccounts...........................................42Managingdepartments............................................43Managingusers..................................................45BDFACSuitepreferences..........................................48SetupandQCoverview...........................................50ImportingoraddingaCS&Tbeadlot................................53AboutsetupandQCreports........................................54BDFACSVerse™SystemUser’sGuideviChapter4:Systemstartupandshutdown59Performingsystemstartup..........................................60Performingmanualsystemshutdown.................................61Performingautomatedsystemshutdown...............................63Chapter5:DailysetupandQC65AbouttheSetupandQCworkspace..................................66DailysetupandQCworkflow......................................70RunningdailyperformanceQC.....................................71RunningAssayandTubeSettingsSetup...............................73Chapter6:Experimentacquisitionandanalysis77Experimentoverview..............................................78Experimentacquisitionworkflow....................................81Creatingandopeningexperiments...................................82UsingtheExperimenttab..........................................85Buildingexperiments..............................................95Creatingtubes...................................................96Creatingplotsinaworksheet.......................................98Previewingdatainplo