避暗实验测定小鼠学习记忆功能方法的研究

6710003。、。。。R338.64A1672-2345201106-0025-03。、、〔1〕。、、〔2〕。、〔3〕。、。11.120±2gSCXK。1.2070811。1.3BA-200。1.420min3mg/kg。1.5180s〔4〕。36v、50Hz3min。MethodologicalStudyofPassiveAvoidanceTestofLearningandMemoryFunctioninMiceWANGYingYANGWenweiLUOJuhuaCollegeofPharmacyandChemistryDaliUniversityDaliYunnan671000China〔Abstract〕ObjectivesToimprovethesensitivityofpassiveavoidancetestthreevarioustestingmethodswereappliedtoestimatethelearningandmemoryfunctioninmicemodelwithdysmnesiainducedbyscopolamine.MethodsScopolaminewasusedtoduplicatedysmnesiamodelsinmice.Latencytimeandnumbersoferrorwereobtainedbysimpletestmethoddoubletestmethodandmulti-testmethod.Thesenstitivitiesofeachmethodwerecompared.ResultsInsimpletesttherewerenosignificantdifferencesbetweenmodelgroupandcontrolgroupinbothlatencytimeandnumbersoferror.Whiledatahavenotshownsignificantdifferenceonlatencytimeindoubletest.Howeverinmulti-testthereweresignificantdifferencesinbothtwoindexescomparedtotheothertwogroups.ConclusionMulti-testwasthemostsensitivemethodtomeasurethelearningandmemoryabilityinanimalduringpassiveavoidancetest.〔Keywords〕passiveavoidancetestlearningandmemorydysmnesiaJOURNALOFDALIUNIVERSITY10620116Vol.10No.6Jun.2011255min〔5-7〕。1.5.119：009：0024h5mint。1.5.219：0015：006h9：005mint。1.5.319：0015：009：0039：0048h35min3t。22.1。1。1x±s2.2P0.05。2。2x±s2.3P0.05。3、4。3x±s4x±s3、。。95%〔3〕。。〔8〕。〔9-11〕。〔4〕。。P＞0.05nP10—100.168/s300.0±0.0279.5±43.3P—0±00.1680.2±0.42nP10—100.026/s289.4±22.5242.1±68.0P—0.1±0.20.0610.8±0.82nP10—100.0321300.0±0.0248.1±102.03d292.9±15.0256.8±44.12278.8±45.0236.1±75.33300.0±0.0286.3±29.0nP10—100.0110±01.10±1.63d0.07±0.140.6±0.5220.2±0.420.5±0.5330±00.2±0.429026P=0.061P=0.026P=0.032P=0.01。3。〔1〕．ADChAT〔J〕．2007234497-501．〔2〕．〔J〕．2003362158－161．〔3〕．〔J〕．200983164-169.〔4〕．〔M〕．3．2002226－834．〔5〕Un-HoJinJang-HyunKimGyu-TaeChangetal.EnhancementoflearningandmemorybyamedicinalformulationSaenhyetanginmice〔J〕.JournalofEthnopharmacology2007109271－280．〔6〕．〔J〕．20031954－6．〔7〕．、〔J〕．2006103499－102．〔8〕．〔J〕．2004231746-48．〔9〕．〔J〕．2010293242－245．〔10〕AlokNahataU.K.PatilV.K.Dixit.EffectofConvulvuluspluricaulisChoisy.onlearningbehaviourandmemoryenhancementactivityinrodents〔J〕.NaturalProductResearch200822161472－1482.〔11〕Xiu-LanSUNHideyukiITOTakayoshiMASUOKAetal.EffectofPolygalatenuifoliarootextractonscopolamine-inducedimpairmentofratspatialcognitioninaneight-srmradialmazetask〔J〕.Biol.Pharm.Bull.20073091727－1731.DXSKYX2010052011-03-03.109027