[分享]岛津公司各种分析方法介绍

目录11.1(1)/GCMS1(2)/GCMS2(3)/3(4)/4(5)/5(6)/(1)6(6)/(2)71.2()/GC81.3/GC91.4(1)/IR10(2)/IR111.5/LC121.6/LC131.7/LC141.8(1)/LC15(2)/LC161.9/LC171.10DL-(1)/LC18DL-(2)/LC191.11/UV201.12B1B2(1)/RF21B1B2(2)/RF221.13LC/LC231.14B/LC241.15E/LC251.16(K)/LC261.17/LC271.18/LC281.19/LC291.20/LC3022.1/GC312.2/GC322.3/GC332.4/GC342.5(1)/UV35(2)/UV362.6(1)/UV37(2)/UV382.7/LC392.8/LC402.9(1)/LC41(2)/LC422.10EDTA/LC432.11P-/LC442.12/LC4533.1()/GC463.2NCI(1)/GCMS47NCI(2)/GCMS483.3(1)/GCMS49(2)/GCMS503.4(1)/GCMS51(2)/GCMS523.5Imazaril/LC533.6N-(1)/LC54N-(2)/LC553.75644.1/GC574.2/GC584.3()/GC594.4()/GC604.5/GC614.6/GC624.7/GC634.8/GC644.9(1)/GCMS65(2)/GCMS664.10(1)/GCMS67(2)/GCMS684.11/GCMS694.12(1)/GCMS70(2)/GCMS714.13(1)/GCMS72(2)/GCMS73(3)/GCMS7455.1(1)/ICP-AES75(2)/ICP-AES76(3)/ICP-AES775.2Pb/AA785.3(1)/LC79(2)/LC805.4Pb(1)/AA81Pb(2)/AA825.5()(1)/ICP-AES83()(2)/ICP-AES845.6(1)/ICP-MS85(2)/ICP-MS865.7/ICP-AES875.8/AA8866.1(1)/LC89(2)/LC906.2/LC916.3()/LC926.4Fumonisin(1)/LC93Fumonisin(2)/LC946.5(1)/LC95(2)/LC966.6/LC97(1)-GCMS1)2)3)1)EICICI(M+1)+1EICIDB-1DB-WAX1InstrumentGCMSQP5000ColumnDB-WAX0.25mm×30mdf=0.25µmCol.Temp60-250(10/min)Inj.Temp250I/FTemp.250CarrierGasHe(100kPa)ReagentGasIsobutane1.1.1C18:0EI1.1.2C18:0CI(2)GCMS1.1.3C20:5EI1.1.4C20:5CI1.1.52779198(3)/GCGC-MS1[]1990(1995)(1995)1.1.612GCGC-MS134GCGC-MS5g()H2O16mLCHCl3MeOH(21)100mL5CHCl35CHCl3MeOH(21)100mL×2CHCl30.5%NaOH100mLNa2SO4()40(4)/2[]1990(1995)(1945)11.720mg()1ml0.5N(MeOH(MeOH)2ml100.55mlNa2SO4+NaHCO3(2+1)30GCGC-MS405ml15mL(5)/3()[](5)(1991)1.1.85g()20%(w/w)KOH(in40%EtOH)15mL8516N-HClpHNa2CO320ml320ml3(30mL20mL20mL3)Na2SO41(6)/(1)4(1)BF3-CH3OH1.1.9(2)H2SO4-CH3OH1.1.1020mg()BF3-CH3OH3mL2n-20ml+20mln-()GCGCMS(30mL20mL20mL3)5020mgH2SO4-CH3OH20mL1n-20ml+20mln-10mLGCGCMS(6)/(2)1.1.11(3)CH2N2(I)(II)50%(III)10mg+2ml(IV)1I2IIN--N’--P-20%0.5-1mL3IIIIII4III10GCGCMS(100mL)(4)DimethylformamideDialkylacetals(CH3)2NCH(OR)25-50mg300µlGCGCMS(6010-15)(5)PhenyltrimethylAmmoniumHydroixide(PTAH)PTAH/(1-1.5M%)30GCGCMSPTAH/INOUT+N--N’--++5%()GC()1.2.1110kPa1.2.2110kPa380kPa[]1)No.G1652)No.15.211.1.11GC-17AAFwCBP200.22mmX25mdf=0.25µm210230230(FID)He100kPa(0.52ml/minat210)Split11001.2.1()1.2.2()[]GC-18APFSCorGC-17AAFwFIDDB-WAX0.25mm×30mmdf=0.25µmAOC-20i/sC-R7AplusorCLASS-GC10[]1)NO.G130GC-17AAFwCBM650.22mm×25mdf=0.01µm50(1min)-(20/min)-240-(6/min)-390390(FID)He(1.5mL/min)1.3.11.3.2[]GC-17AAFw+OC1-17FIDULTRAALLOY650.53mm×30mdf=0.1µmAO-20i/sC-R7AplusorCLASS-GC10(1)-IR(LC)(GC)µgFTIRLC-FTIRLC1.4.1UVACLCKBr1.4.2A1.4.3B1730cm-11.4.4CInstrument:LC-VPColumn:Shim-packSCR-102H(8mmφ×300mmL)MobilePhase:5mMTrifluoroaceticAcidAqueousFlowRate:1mL/minColumnTemp.:50Detector:UVD(absorbanceat210nm)Instrument:FTIRResolution:4cm-1Accumulation:50Appodization:Happ-GenzelDetector:PyroelectricDetector(2)-IR1.4.1LC1.4.2A(T)1.4.3B(G:)1.4.4C()δ(10-HDA)[]()(/)0.45µmSTRODS-II(4.6mm150mm)10mM/=55/45(V/V)1.0mL/min40=210nm1.5.1(210nm)ADAM(9-An-thryldiazomethane)ADAM[]Shim-packCLC-ODS(6.0mm150mm)/=95/5(V/V)1.0mL/min45(Ex365nmEm415nm)1.6.11.6.2ADAM[]1.6.3ADAM1234561234[]49(1)59(1992)No.18310-11()Shim-packSCR-102H2(8.0mm300mm)5mMvp0.8mL/min455mMBis-Tris20mMBis-Tris100µmEDTA0.8mL/min481.7.21.7.1123456789(1)LC()OPA()[]ApplicationReportNo.19()Shim-packCLC-DOS(6.0mm150mm)Shin-packGRD-ODS(4.0mm250mm)(A)10mM(pH6.8)(B)A/=2/1(C)80%1.0mL/min45E350nmEm460nm(1)E485nmEm530nm(2)1.8.1min(2)LC[]Pre-treatmentSample10-20µLβ-Mercaptreagent1)200µLOPAreagent2)200µLMixingNBD-Clreagent3)200µLMixingandWaitingInjection10µLtoHPLC1)β-Mercaptpropionicacid10µL100mMBoratebuffer(pH9.0)10mlL2)OPA20mgAcetonitrile3mL100mMBoratebuffer(pH9.0)10ml3)NBD-Cl100mgAcetonitrile10mL1.8.2TimeFunctionValue0.01B.CONC54.0B.CONC158.0B.CONC2016.0B.CONC2718.0B.CONC3025.0B.CONC4530.0B.CONC5039.0B.CONC6539.01B.CONC7042.0B.CONC7548.0B.CONC8048.01B.CONC10049.0SV153.0SV054.0B.CONC10054.01B.CONC054.02STOP1.8.3()OPA10N-2179.1.2[]No.17No.L19647(4)365(1990)50010mLShim-packAmino-NaNAB600.5mL/min(E×348nmEm450nm)A/BOPAN-/AB0.2mL/min1.9.1171.9.2(1)LCLCOPA/N[]N.NimuraandT,KinoshitaJ.Chromatogr.,352,169(1986)2pp716.21(1992)1pp101(1993)No.L235DevelosilODS-UG-5(6.0mm150mm)Shim-packGRD-ODS(4.0mm250mm)(A)50mM(B)(A)(B)1.2mL/min35E350nmEm450nm1.10.1DL-(2)LC[]1.10.21.10.31.10.4TIMEFUNCTIONVALUE16BCONC2424BCONC2429BCONC4050BCONC4059BCONC6759.01BCONC8064BCONC8064.01BCONC065STOPStandardsolution300µLbuffersolution*1200µLmixreagentA*2100µLmixreagentB*3100µLmixandwai