不同相对分子量透明质酸功能及应用的研究

CHINESEJ.DIAL.&ARTIF.ORGANSVol.22NO.2JUN.2011510006,,【】Mr。HA、、、、、、。HA(LMWHA)HA(o-HA)、、、HALMWHAo-HA。【】R965A1005-0809(2011)02-0010-04ResearchontheFunctionandApplicationofDifferentMolecularWeightHyaluronicAcidHUANGYue-shan,PANYi-ming,XUEJing(BiologicalScienceandEngineeringCollege,SouthChinaUniversityofTechnology,Guangzhou510006,China)【Abstract】Hyaluronicacidisakindofmacromolecularmucopolysaccharide,whichbiologicalactivityandusingeffectassociatewithitsmolecularweight,soweshouldbedependingonthedifferentpurposes,selecttheappropriatemolecularweight.Thispaperreviewstheprogressonthefunctionandapplicationofdifferentmolecularweighthyaluronicacidinrecentyears,detailingthehighmolecularweightHA(HMWHA)hasbeenwidelyappliedinthefieldsoftreatmentofosteoarthritis,postoperativeanti-adhesion,viscoelasticagentophthalmicsurgeryandeyedrops,becauseofitscharacteristicsingoodviscoelasticity,water-holding,suppressinflammationresponse,lubricate,drugsslowreleaseandsoon;lowmolecularweightHA(LMWHA)andHAoligosaccharides(o-HA)havethefunctionofanti-tumor,promotingwoundhealing,promotingangiogenesis,immuneregulationandsoon,andbythedegradationofHAcangettheavailableLMWHAando-HA.【Keywords】hyaluronicacidmolecularweightfunctionapplication(hyaluronicacid,HA)(ECM)。、、、[1]、、。HA。HA(HMWHA)Mr2×106HA、、、Mr(1~2)×106HA、、、Mr(1~8)×104HA(LMWHA)Mr1×104HA10--20116222(o-HA)、、、[2]。HMWHA、LMWHAo-HA。1HMWHA1.1、[3]。[4]。Balazs[5]2060。。[3]HMWHALMWHAAHMWHAMr6～7×106、BLMWHAMr5～7×105、C10,、。、。ABCP0.05BAP0.05。ββ。1.2HAHAMr。[6]AMr2.5×106、BMr1.0×106、CMr0.5×106D3、5、8n=6A、BDP0.01CDP≥0.01Mr2.5×106HAMr1.0×106HAMr0.5×106HA。[7-8](1)。。(2)。、。1.3、。[9-11]。3×106MrHA。、、。HAMr(1.0~1.5)×106MrHA[12](1)HA(2)HA(3)HA。。、。[3]。2LMWHAo-HA2.1[13]。———CD44RHAMM11--CHINESEJ.DIAL.&ARTIF.ORGANSVol.22NO.2JUN.2011。[14-19]。Misra[20]MCF-7/AdrHACD44ErbB2-3MCF-7/Adr-3Akt。3～10o-HAMCF-7o-HACD44-3。2.2、、。[21]o-HAHA。o-HA。o-HAHA,[22-24]。HAo-HACD44RHAMM(receptorforHA-mediatedmotility)[25]。[26]、MTT、o-HA。o-HACD44o-HAERK-1/2RHAMMo-HAERK-1/2。o-HARHAMM。2.3[13]HMWHALMWHA。HMWHALMWHA。[2]HA(46)Mr(0.8~2)×105HA。HA-4(TLR-4)。Termeer[27]800~3200o-HA、8×104~2×105HALMWHAo-HAHALMWHAo-HA、[28]。2.4HA、HA、、HA。HA123LMWHAo-HA4。LMWHAo-HAHALMWHAo-HA。HA。[2]Mr5×105HANF-kB/-kB、HA。HA、、、。LMWHAo-HA。3。HMWHA、、、12--20116222。HALMWHAo-HA、。LMWHAo-HALMWHAo-HA。[1],,.[J].,2008,12(23):4515-4518.[2],,.[J].,2005,7(12):1-3.[3].[J].,2008,30(4):340-341.[4]GhoshP,GuidolinD.Potentialmechanismofactionofintraarticularhyaluronantherapyinosteoarthritis:aretheeffectsmolec-ularweightdependent[J].SeminarsinArthritisandRheumatism,2002,32(1):10-37.[5]BalazsEA,DenlingerL.Viscosupplementation:anewconceptinthetreatmentofosteoarthritis[J].JournalofRheumatologySup-plement,1993,39:3-9.[6].[J].,2002,19(4):334-336.[7]GaughanEM.Effectsofsodiumhyaluronateontendonhealingandadhesionformationinhorses[J].AmerricanJournalofVet-erinaryResearch,1999,52(5):764.[8]DavidAmiel:Hyaluronaninflexortendonrepair[J].TheJournalofHandSurgery,1999,14A(5):837.[9]GreenbergDD,StokerA,KaneS,etal.Biochemicaleffectsoftwodifferenthyaluronicacidproductsinaco-culturemodelofos-teoarthritis[J].OsteoarthritisCartilage,2006,14(8):814-822.[10]GuechotJ,EdriefS,LasnierE,etal.Automatedassayofhyaluron-icacidinserum[J].Immuno-analyseBiologieSpecialisee,2008,23(3):148-152.[11]CampoGM,AvenosoA,CampoS,etal.Theantioxidantandan-tifibrogeniceffectsoftheglycosaminoglycanshyaluronicacidandchondroitin-4-sulphateinasubchronicratmodelofcar-bontetrachloride-inducedliverfibrogenesis[J].Chemico-Bio-logicalInternactions,2004,148(3):125-138.[12],.[C].8,,2004:87-91.[13],,,.[J].,2006,8(3):6-9.[14]ChoiKY,LeeS,ParkK,etal.Preparationandcharacterizationofhyaluronicacid-basedhydrogelnanoparticles[J].JournalofPhysicsandChemistryofSolids,2008,69(5-6):1591-1595.[15],,,.HAV[J].,2008,24(2):183-185.[16]ScheibnerKA,LutzMA,BoodooS,etal.HyaluronanfragmentsactasanendogenousdangersignalbyengagingTLR2[J].TheJournalofImmunology,2006,177(2):1272-1281.[17]TayE,OlverJ.Intraorbitalhyaluronicacidforenophthalmos[J].Ophthalmology,2008,115(6):1101-1101.[18]KuttyJK,ChoE,SooLeeJ,etal.TheeffectofhyaluronicacidincorporationonfibroblastspreadingandproliferationwithinPEG-diacrylatebasedsemi-interpenetratingnetworks[J].Bio-materials,2007,28(33):4928-4938.[19]LiuL,DuG,ChenJ,etal.InfluenceofhyaluronidaseadditionontheproductionofhyaluronicacidbybatchcultureofStrepto-coccuszooepidemicus[J].FoodChemistry,2008,110(4):923-926.[20]MisraS,GhatakS,TooleBP.RegulationofMDR1expressionanddrugresistancebyapositivefeedbackloopin-volvingyaluro-nan,phosphoinositide3-Kinase,andErbB2[J].TheJournalofBiologicalChemistry,2005,280(20):20310-20315.[21]PhamHT,BlockNL,LokeshwarVB.Tumor-derivedhyaluroni-dase:adiagnosticurinemarkerforhigh-gradebladdercancer[J].CancerResearch,1997,57(4):778-783.[22]HallCL,LangeLA,ProberDA,etal.App60(c-src)isrequiredforcelllocomotionregulatedbythehyaluronanreceptorRHAMM[J].Oncogene,1996,13(10):2213-2224.[23]RaoCM,DebTB,DattaK.Hyaluronicacidinducedhyaluronicacidbindingproteinphosphorylationandinositoltriphosphateformationinlymphocytes[J].Biochemistry&MolecularBiologyInter