马铃薯品质性状分子育种研究进展

!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!![1]#######$#(75%)[2]#!DNADNADNA#DNA%DNA!!###$#$###!!![3~5]##[6~10]#2090##!.DNA#!&863’&’(2004AA241131)!##1967##()###10%161606()#Tel%0452-4527329#E-mail:[e-mail]!2005-01-24#%2005-02-01!!!(#161606)!!!!!#!$$$#$%&$’((%)*’(’+$,-%.#%/+/%0%1’,21+$3$’’45.&)%$62+15/5’(8&7,9:1,;0,:1,9=0/&4,=07,9?4,@04A&0;0,B!#$#%&’&$()*+,’#-#.#&/0&-1,23-4,4)45&67/82(-).1#.(&9)-&,)&’C7D&1,##$#E78(/$+,/9F&0D(1(75-507G6?0,’53H4/7D’&7/4557,’57D715/&1,HH7I763(;7,’3G(3’1’3;367/4615-577H0,9G35J4160’07D.K,’&7;71,’0;7’&7(57D7,’(53-67;D3G’&7;367/4615;15L7557D715/&1,H97,7’7/&,363!907D157-70,9H0D/4DD7H.M,H’&7G4’457H7I763(;7,’3G(3’1’3;367/4615-577H0,9’7/&,363907D0D16D3(53D(7/’7H.:’;%$4(9N3’1’3O03’7/&,3639?P4160’?Q&151/’75!#**ChineseAgriculturalScienceBulletinVol.21No.62005June!#$%%&’()*+,’-./01’-2)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3!RFLP#$DNA$%&PCR#!DNA&RAPD#$&AFLP#$&STM#!!’&SNP#$&EST#[11~16]%7)8)8DavidsSDouches&1994#RFLPs$RAPD%F1&Haploids!#$%&’()$*!!+,-+(&.)&#!!/,$’&0-!!!QTL!QTLs%12RAPDs!6QTLs!2!4!5!10[11]%%7)8)7!%19!8!!%!40ku&!Patatin!40%#%!!!%40ku!10~15!40~60%4kb%!6%5(GT/3(AG%5(22bp!12!!98%~99%!!1%~2%%&RFLP#!140DNA[12~15]%7)7!!!!!!%7)7)8!’!)%ADP-!%MosantoADP-!24%%!!2%!30%8%%!!0.001%~0.01%%![17]%7)7)7!%*%!!%!*!!![17]%!!:(1)!AGPase!AGPase!%!!!)(2)%!RuthLor-berth%(3)!!%!!%!!!!%!#++!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,!#!$$%#&’’()**+,’-./&0,12345,16.,7’./,![17]!.!.812240ku5#$2.5ku%TPB1101.1!-$GUS%!LBA4404!!&!.!.9!!&ADPG!Monsanto&!#$%&ADPGglgc’glgc16!RussetBurbank!!60%!!glgc16!8[17]!.!.%!!T-DNA!!!T-DNA!!((((()1*&RNA[1,2,5]&!8.$1%+$((%[17]&$2%+!$QTL%[18]&$3%+!&Tanksley&Nelson(1996)QTL$advancedbackcrossQTLanalysis!AB-QTL%!QTL[15,18,19]&$4%!200!0.05~0.5!QTL&!,![20,22]&8.!$1%![23~26]&$2%[27,28]&$3%!![29]&$4%!+!)*&!&!!&!&!)*&DNA$%!!!DNA&!!&8%~10%![17]&&!!!&!!&1..,!!--ChineseAgriculturalScienceBulletinVol.21No.62005June!#$%%&’()*+,’-./01’-2)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3ChineseAgriculturalScienceBulletinVol.21No.62005June+5$%%&3()*+,3-./013-6)34)*3(I1)!(II)!(IV2)!#(I2)#(III1)!$!3.1()!$%!!!!&!!!!&3.2!!!!!()!()!!!&3.3%%!%!!!!%&!!!&1,,.().,2004,19():27~322,.().,2005,(1):257~259,2953..:,1988.124~1264..:,2004.14~1235..,1989,(3):1~31993.6~122,,..,1990,(2):23~283,..:,1992.62~804,..,1998.375,,,..21,2000:103~1076,..,1996,(1):49~547,,.QTL.,2004,(1):77~838,..,1998,(5):31~329,..,2001,(3):165~16910..,1998,(1):4~811BernardoRA.modelformarker-assistedselectionamongsinglecrosseswithmultiplegeneticmarkers.Theor.Appl.Genet,1998,(97):473~47812CaiQ,GuyCL,MooreGA.ExtensionoflinkagemapinCitrususingrandomamplifiedpolymorphicDNA(RAPD)markersandRFLPmap-pingofcold-acclimation-responsiveloci.TheorApplGenet,1994,(89):606~61413DeVicenteMC&SDTanksley.QTLanalysisoftransgressivesegrega-tioninaninterspecifictomatocross.Genetics,1993,(134):585~59614DouchesSD,RosannaFreyre.Identificationforgeneticfactorsinfluenc-ingchipcolorindiploidpotato(solanumssp.).AmPotatoJournal,1994,(71):581~59115DudleyJW.Molecularmarkersinplantimprovement:manipulationofgenesaffectingquantitativetraits.CropSci,1993,(33):660~66816ChenHH,PHLi.Characteristicsofcoldacclimationanddeacclima-tionoftuber-bearingSolanumspecies.PlantPhysio,1980,(65):1146~114817..:,2003.283~34018EathingtonSR,JWDudley&GKRufenerII.Usefulnessofmarker-QTLassociationsinearlygenerationselection.CropSci,1997b,(37):1686~169319FernandoRL&MGrossman.,Marker-assistedselectionusingbestlinearunbiasedprediction.Genet,1989,(21):467~47720HanF,RomagosaI,UllrichSE,JonesBL,HayesPM&WesenbergDM.Molecularmarker-assistedselectionformaltingqualitytraitsinbarley.Mol.Breed,1997,(3):427~43721HospitalF,MoreauL,LacoudreF,CharcossetA&GallaisA,Moreontheefficiencyofmarker-assistedselection.Theor.Appl.Genet,1997,(95):1181~118922KatsubeT,Kazuta.UDP-glucosepyrophosphorylasefrompotatotuber:cDNAcloningandseq