06-Bt杀虫晶体蛋白受体分子的结构与功能

ActaEntomologicaSinica,December2006,49(6):1009-1016ISSN045426296:(30471159,30270900);973(2006CB102000):,,1978,3Authorforcorrespondence,E-mail:[e-mail]:2005-09-04;Accepted:2006-05-20Bt1,2,2,1,1,1,3(11,100081;21,100094):(Bacillusthuringiensis,Bt)BtBtBtBt,,Bt4:NBt:;Bt;;;:Q966:A:045426296(2006)0621009208AdvancesinresearchonmolecularstructureandfunctionofBt(Bacillusthuringiensis)receptorsininsectsWANGLi1,2,LIXue-Feng2,XUBao-Ren1,WUQing-Jun1,ZHANGYou-Jun1,3(11InstituteofVegetablesandFlowers,ChineseAcademyofAgriculturalScience,Beijing100081,China;21CollegeofScience,ChinaAgriculturalUniversity,Beijing100094,China)Abstract:Bacillusthuringiensis(Bt)receptorisveryimportanttothetoxicityexpressionofBtininsects.ChangeinbindingabilitybetweenthisreceptorandBtinsecticidalproteinsmaybethemainmechanismofBtresistance.Inthispaper,thetypes,structure,bindingcharacteristicsoftheinsectreceptors,includingaminopeptidaseN,cadherin-likeprotein,alkalinephosphataseandglycolipids,theexpressionofgenesofinsectreceptorsinendogenouscells,andtherelationshipsbetweenBtresistanceandreceptorarereviewed.Keywords:Insect;Bacillusthuringiensis;receptor;structure;functionBacillusthuringiensis(Bt),95%;Bt2004,Bt2240(Zhaoetal.,2005),,,BtBt,BtBtPlutellaxylostellaTrichoplusiani,Bt1260,(GriffittsandAroian,2005):Bt,,,(brushbordermembranevesicles,BBMV)(Cry),(Danieletal.,2002)Bt,(2003)34,,BtBtN(aminopeptidaseN,APN)(cadherin-likeprotein)(alkalinephosphatase,Alp)(glycolipids)1N(APN)N,55%(Losevaetal.,2002),111NN,Manducasexta(BBMV)120kDAPNAPN31%31%31%29%(Knightetal.,1995)N,Nakanishi(2002)Bombyxmori8APN,,8APN4,APN1APN2APN3APN4HelicoverpaarmigeraLymantriadisparAPN420061,GenBankAPN60,33,(2003),112Lorence(1997),APN(glycosylphosphatidylinositol,GPI)APNAPN,APN1(Yaoietal.,1999)APN3(Gomezetal.,2001)Cry1AaIle135Pro1984APN(APN1APN2APN3APN4)Cry1AaCry1Ab,4APN,Cry1AaCry1AbAPN,APN(Nakanishietal.,2002),APNAPN,Rajagopal(2003)APN(HaAPN1HaAPN2),APNCry1AaCry1AbCry1Ac:3APN1Cry1AcAPN2APN113,APN(1)APNBBMV,EpiphyaspostvittanaAPNSf9130kD,,GPISpodopteralituraAPN,,APNCry1Ac,,APNCry1Ac(GillandEllar,2002)APNSf9Cry1Ac,Sf9,Sf9,Cry1AcCry1BaBtHeliothisvirescensAPNCry1AcCry1Fa()S2,S2,Cry1AcAPNS2,APN,,APNBt(Banks,2003)1NTable1ExpressionofaminopeptidaseNgeneoninsectmidgutinendogenouscellsInsectspeciesEndogenouscellsReferencePlutellaxylostellaSf9Denolfetal.,1997BombyxmoriE.coliYaoietal.,1999ManducasextaSf21Louetal.,1999LymantriadisparSf9Karneretal.,1999EpiphyaspostvittanaSf9SimpsonandNewcomb,2000SpodopteralituraSf21Agrawaletal.,2002HelicoverpaarmigeraTrichoplusianiRajagopaletal.,2003HeliothisvirescensDrosophilaS2Banksetal.,2003ManducasextaDrosophilaGillandEllar,2002114BtZhu(2000)PlodiainterpunctellaBtHD198rRC668s,cDNA,0101ActaEntomologicaSinica494,2PCR(PASA)BtHD198r,Asp185Glu185,Cry1A(Cry1Aa),,Rajagopal(2002)RNAAPNCry1C,APNBtCry1CKnight(2004)120kDAPN,120kDAPNCry1AcWang(2005)BtCry1AcBBMVBBMVAPNBtAPNBtLee(2000)Cry1AbCry1Ac2,,Cry1AbBBMVAPN,Morin(2003),BBMVCry1AN,Banks(2003)Cry1Ac110kD,2Vadlamudi(1993)BBMVCrylA(cadherin),(Midboeetal.,2003)211()Bt-R130%60%20061,GenBank821,12,N,C,,110PectinophoragossypiellacDNAcDNA50006000bp,1700,,175270kD,210kDcDNANC:N,(repeat,912)(Bt),,2126(Gahanetal.,2001;Dorschetal.,2002;Huaetal.,2004a)212Bt,017310nM,N()Cry1BtBt-R175CR9,Bt-R1aCR12(Huaetal.,2004a)Bt-R1Cry1A(Gomezetal.,2003)Cry1Ab1(TBR1)()7(CR7)7(Gomezetal.,2002);2(TBR2)()11(CR11)(Huaetal.,2004a)Hua(2004a)Bt-R1Bt-R1a,Bt-R1a()CR11CR12Cry1Ab,CR12Bt-R1aCry1AbNagamatsu(1999)Bt-R175NBtR175Cry1AGomez(2001)Bt-R1Bt-R175Cry1A,869HITDTNNK8761296LDETTN1301Bt-R1Bt-R175Cry1A,11016:Bt,Cry1AcCry1AbCry1Aa,,Cry1AaCry1AbCry1AcCry1Ja,,Cry1A(Gonzalez-Cabreraetal.,2003)213,4(2)Bt-R1cDNA,,SpodopterafrugiperdaSf21Cos7Hek293,195kD,BBMV15kD(KeetonandBulla,1997),Bt-R175cDNA,Bt-R175,(Nagamatsuetal.,1999)Tsuda(2003)Bt-R175Cos7Hek293,Cry1AaCry1AbCry1Ac,Cry1AcBt-R1752Table2Expressionofcadherin-likegeneoninsectmidgutinendogenouscellsInsectspeciesEndogenouscellsReferenceBt-R1Sf21KeetonandBulla,1997;Mengetal.,2001ManducasextaBt-R1Cos7KeetonandBulla,1997;Dorschetal.,2002Hek293KeetonandBulla,1997Bt-R175Sf9Nagamatsuetal.,1999BombyxmoriBt-R175Cos7Ikawaetal.,2000Hek293Tsudaetal.,2003Bt-R1(a)ManducasextaBt-R1(a)DrosophilaS2Huaetal.,2004bOstrinianubilalisSf9Flannaganetal.,2005214Bt5Cry1Ac,(Xuetal.,2005),Gahan(2001),Cry1Ac10128YHD2Cry1A,C1110YHD23mRNA5,,,Morin(2003),Cry1Ac(3100)3(r1r2r3)82,1(rs)(ss)(Huaetal.,2004a)Cry1Ab13631464(Huaetal.,2004a)1430GlnAlaCry1Ac;1425Leu,CTGCGG,(Huaetal.,2004a)Bt,Xu(2005)Cry1Ac,DNABtBt,Cry1Aa,N(Jenkinsetal.,2000)2101ActaEntomologicaSinica49,AFLPPxCaLPLG8Mode1LG22,,BtDNA,(Baxteretal.,2005)3(Alp),311,Alp,(membrane-bound,mAlp)(solubleform,sAlp)2Alp;60kD;L-(Eguchi,1995)mAlpsAlpmAlp,,sAlp(Eguchi,1995)3mAlp,(Itohetal.,1999),mAlp,cDNA1974bp,547,36511mAlp13Alp,310Zn2+Mg2+(Itohetal.,1999)CmAlpN-(GalNAc),GPIBBMV,Bt,mAlp,(Jurat-FuentesandAdang,2004)312BtBravo(2002)WesternCry1A,Cry1Ab,APNAlp,McNallAdang(2003)APNCry1AcWesternNCry1Ac,mAlpCry1AcCry1Ac,Cry1Aa,Cry1AbCry1Ac(Jurat-FuentesandAdang,2004)Cry1AcAlp,mAlpCry1Ac,Cry