流式细胞仪入门

-----OOO1233.13.244.14.24.34.455.15.25.35.466.177.17.28FACScanTMFACSortTMFACSCaliburTMBDLSRFACSVantageTMFACSVantageTMSEFACStarPLUSTM0.2-150Listmode1-11234567892-12-22-12-22-1BDLSR2-2DNA123456789DNA10113.1FSC3-1FSCSSC3-1SSC903-13-2FSCSSC3-2FSCSSC1234FSC5906SSC73.2488nm7FITC3-3488nmFITCFITCFITC3-3FITCPEPerCPAPC488nmFITCPE3-4PE488nmFITC530nmPE570nm3-4FITCPEPerCPAPC3-53-5FSCSSC12345FITCPE67---4.14-14-2FACSCaliburBDLSR4-1FACSCalibur4-2BDLSR4-34-3FACSVantageSE12344.2SSCPMTsFSCPMTsPMTsBPFITC530/30530±15nm515nm545nmBP500/50475nm-525nmLPSPLP500500nm500nm4-45604-1560nm560nm4-1560nm4-412530/30344.3BDPMTsPMTsSSCFSCPMTs4-54-5LinLogLogLin0-1000mV0-1,000mVGPIO4-64-61FSC2SSC34ADC4.4FSC1FSC25.1FSC“”FSC4FSCSSCFITCPE810000FCS80kBFSCFITCFL15-15-1X1FL1Y2FL23XYZ5-1123CellQuest3Z5.2FSCFSCSSC5-215.3list-mode,5-35-35-4M1M2CD3FITC5-4M1NORM001CD3FITCM2NORM0025-560002891M1619M2CD32272CD3M22272/2891=78.59%5-55-6LLULYCD19PELRXCD3FITCURCD19+/CD3+5-6(NORM001)CD3FITC/CD19PE(NORM002)5-7CD19+/CD3+296/2839=10.43%5-75-85-9R4CD4CD340/2866=1.40%5-8CD3FITC/CD4PE5-9CD3FITC/CD4PEclusteranalysisBDMultiSETTMAttractorsTM5-105-10Attractors5.4100%100%5-1125205-11QuantiCALCTM5-12Y5-12QuantiCALCModFitLTTMDNADNAG0/G1SG2M5-13DNA125-45-5CD33LL45-65-7CD3+/CD19-56786.1FACSCalibur300FACSCalibur6-1FACSVantageSEFACSVantageSE6-16-2FACSVantageSE12FACSVantageSE3FACSCalibur4FACSVantageSELASERlightamplificationbystimulatedemissionofradiation.19647-1488nm7-1635nm7.21488nm23411234567892123456789101133.1123FSC45SSC67FSCSSC3.212345488nm6FITCPE744.112344.212515545344.312344.41255.11235.215.41221.41%78.593479.57%5678612347123635nm4