BD新产品通讯

•ClicktoeditMastersubtitlestyle2BD信息产品信息•干细胞分选、鉴定试剂盒•凋亡、周期、增殖一步法测定KIT•BD新紫外染料---V421•免疫领域新Marker---TregSTAB13BDBiosciencesStemCellResearch–CellAnalysis干细胞PanelKit•BDStemflowKits:PluripotentStemCells•BDStemflowKits:MesenchymalStemCells•BDStemflowKits:NeuralStemCells•BDStemflowKits:HematopoieticStemCells4BDBiosciences5BDBiosciences6KeyStemCellMarkersTranscriptionfactorthatmarksforpluripotencySox2Transcriptionfactorthatcontrolsembryonicstemcellself-renewalandpluripotency.NanogTranscriptionfactorthatcontrolsembryonicstemcellself-renewalandpluripotency.Oct3/4TumorRejectionAntigenanditsexpressiononhumanpluripotentstemcellsTRA-1-81TumorRejectionAntigenandisasurfacemarkerthatisexpressedonhumanpluripotentstemcellsTRA-1-60Cellsurfacemarkerthatmarksforpluripotencyanddifferentiation.SSEA-4Cellsurfacemarkerthatmarksdifferentiation.SSEA-3Stage-SpecificEmbryonicAntigen:asurfacemarkerthatmarksforpandifferentiation.SSEA-1SignificanceMarker7BDStemflowTMKits:Pluripotentstemcells(ESandiPScells)mNanogPEOct3/4PerCP-Cy™5.5Sox2AlexaFluor®647MsMousePluripotentStemCellTranscriptionFactorAnalysisKit(Cat.No.560585)•AllpluripotentstemcellkitscontainBD™CompBeadPluscompensationparticles•Isotypecontrols•Intracellularanalysiskitscontainfixandpermbuffers•50testsGFPDrop-insSortingIntracellularAnalysisCellSurfaceAnalysisAntibodiesSpecieshNanogPEOct3/4PerCP-Cy™5.5Sox2AlexaFluor®647SSEA-1PEOct3/4PerCP-Cy™5.5SSEA-4AlexaFluor®647SSEA-1FITCSSEA-3PETra-1-81AlexaFluor®647HuHumanPluripotentStemCellTranscriptionFactorAnalysisKit(Cat.No.560589)Hu/MsHumanandMousePluripotentStemCellAnalysisKit(Cat.No.560477)HuHumanPluripotentStemCellSortingandAnalysisKit(Cat.No.560461)特点：方便、稳定、全面8BDBiosciences9MesenchymalStromal(Stem)Cells(MSCs)•MSCs可分化形成多种组织细胞–骨,软骨cartilage,肌肉,脂肪组织,以及骨髓基质•MSCs可在体外分离培养•MSCs应用–再生医学–疾病的体外模型DrugscreeningToxicologyBasicresearch10BDStemflowTMKits:HumanMSCAnalysisKitAppNoteAppNoteGFPGFPDropDrop--insinsSortingSortingIntracellularIntracellularAnalysisAnalysisCellSurfaceCellSurfaceAnalysisAnalysisAntibodiesAntibodiesMSCPositiveCocktail:CD105PerCP-Cy5.5/CD73APC/CD90FITC+CD44PEMSCNegativeCocktail:CD45/CD34/CD11b/CD19/HLA-DRPEHumanMSCAnalysisKit(Cat.No.562245)50tests•完全符合ISCT推荐的Panel（人骨髓来源的MSCs）–DominiciM,et.alMinimalcriteriafordefiningmultipotentmesenchymalstromalcells.TheInternationalSocietyforCellularTherapypositionstatement.Cytotherapy.2006;8(4):315-7.•只需4色的机器(eg.BDFACSCalibur)即可完成，但可随时加入其它Marker扩展到5色及以上11SalesTools:ProductDataSheet12BDBiosciences13(NSC)•在胚胎发育过程中发现并局限于成人的脑•NSCs可被分离并体外培养–胎儿和成人的脑–hESCs分化而来•NSCs应用–移植治疗–人体发育的体外模型–人类疾病的体外模型DrugscreeningToxicologyBasicresearchForResearchUseOnly.Notforuseindiagnosticortherapeuticprocedures.NeuralStemCells(NSCs)14BDStemflowTMKits:Multipotentstemcells-NSCsAppNoteAppNoteGFPGFPDropDrop--insinsSortingSortingIntracellularIntracellularAnalysisAnalysisCellSurfaceCellSurfaceAnalysisAnalysisAntibodiesAntibodiesCD24PECD271PerCP-Cy™5.5CD44PerCP-Cy™5.5CD15PE-Cy7CD184APCHumanNeuralCellSortingKit(Cat.No.562271)20testsCD44FITCKi-67AlexaFluor®488DoublecortinPESox1PerCP-Cy™5.5Sox2PerCP-Cy™5.5GFAPAlexaFluor®647NestinAlexaFluor®647HumanNeuralLineageAnalysisKit(Cat.No.561526)25tests与LarryGoldstein‘slab（UCSD）合力之作,BD独有的能分离活的NSCs（来源于胚胎干或者iPS),为研究NSCs的科研工作者提供了一个划时代的标准。Kit包含优化的方案、试剂及对照，稳定性极高。BDStemflow™NeuralCellSortingKit小包装，方便易用，随时可加入其它Marker.BDStemflow™NeuronalAnalysisKitDescriptionProduct15HumanNeuralLineageAnalysisKit•可以随时监测从胚胎干到神经细胞或神经胶质的分化状态BuffersIncludedGlialcells,astrocytes,embryonicandneuralstemcellsFilament25/NestinNestinCytofixPermIIIImmaturepost-mitoticneuronsNeuronfilament30/DoublecortinDoublecortinAstrocytesFilament1B4GFAPAllproliferatingcelltypesNuclearproliferationmarkerB56Ki-67GlialcellsandneuralstemcellsTranscriptionFactorN23-844Sox1Glialcells,embryonicandneuralstemcellsTranscriptionFactorO30-678Sox2Glialcells,astrocytes,astrocyteprecursorsH-CAMG44-26CD44NeuralCellPopulationIdentifiedMoleculeCloneSpecificityYuanetal.Cell-SurfaceMarkerSignaturesfortheIsolationofNeuralStemCells,GliaandNeuronsDerivedfromHumanPluripotentStemCells.PLoSONE.March2;6(3):e17540.:ProductDataSheets17BDBiosciences18BDStemflowTMKits:HematopoieticStemCellsAppNoteAppNoteGFPGFPDropDrop--insinsSortingSortingIntracellularIntracellularAnalysisAnalysisCellSurfaceCellSurfaceAnalysisAnalysisAntibodiesAntibodiesCD177(c-Kit)PECD34FITCSca-1PE-Cy7LineagecocktailAPCHumanMouseHSCIsolationKit(Cat.No.560492)100tests(10sortsofthebonemarrowpooledfrom10mice)•利用流式细胞仪基于经典的KLS(c-Kit+Lin-Sca-1+)表面Marker分选•为提高产量和稳定性，Kit含有滴定好的直标的代表自我更新能力的Marker(c-KitPE,Sca-1PE-Cy™7),代表造血祖细胞的marker(CD34FITC),以及造血系统的hematopoieticlineage(APCLineageCocktail).•Kit还提供MouseBDFcBlock™,7-AADvitaldye,andisotypecontrols，BD™CompBead.•优化的Protocol.19Q&A•主要研究的干细胞：–ESC/iPS，MSC，NSC，HSC•可分析/分选ESC/iPS的Kit–HumanPluripotentStemCellSortingandAnalysisKit(Cat.No.560461)–HumanandMousePluripotentStemCellAnalysisKit(Cat.No.560477)–HumanPluripotentStemCellTranscript