细菌细胞壁肽聚糖的研究-刘芳

246201112JournalofSichuanUniversityofScience＆EngineeringNaturalScienceEditionVol.24No.6[e-mail]1673-1549201106-0628-04643000。、、、、、。Q936B、、、。、、、、G+30%～95%G－5%～20%。、、、、、、。1N－NAGN－NAM1。3①N－β－14N－。②4N－。G+3StaphylococcusaureusL－Ala→D－Glu→L－Lys→D－AlaCorynebac-teriumpoinsettiaeL－Ser→D－Glu→L－Orn→D－Ala。G－1∶1∶1.5～2.0D－Glu、m－DAPDL－AlaEscherichiacoliL－Ala→D－Glu→m－DAP→D－Ala。③2“”100。G+S．aureus—Cly5—。G－—CO·NH—G+。2、。G－G－G－。G+。SchleiferKan-dler1972G+①NA、B34A24B。BA。②AA1、A2、A3、A4BB1、B2。③3A2α、β、γA1α、A1β、A1γB1α、B1β、B1γ2。3。3ParkSB900SB9009.84%NAG0.871μmol/mgNAM1.14μmol/mgAla、Glu、Asp1.046、0.775、0.304μmol/mg。48g/dLSDS10minSDSTCA。Ala、Asp、GluLys1.1810.9430.7700.456mmol/g。5、、15.8%。4G+S．aureus20、3。①N－UDP－NAGN－UDP－NAMN－“Park”UDP－NAM－。N－－1－N－、N－“Park”UDP。②“Park”N－、。。③。21。D－－D－2。D－－D－“Park”。。5、、、、、、6。。Sekine71IL－1、6IL－6αTNF－αmRNA。8IL－1、IL－6TNF－α。9926246。10。11SB900。12。13200mg/kg60d。。Ishihara14。Sekine15MethA。SunJ16。17。18Lb．casei．ZhangBGC－823、H22Lb．casei．Zhang。19。6、、、、。、。。1．M．2002．2．J．1992193174-179．3．SB900J．1998385376-380．4．J．200322650-54．5．J．2008304605-609．6．J．200438138-40．7SekineKOhtaJOnishiMetal．Analysisofantitu-morproperiesofefectorcellsstimulatedwithacellwallpreparationWPGofbifidohacteriuminfantisJ．BiolpharmBull1995181148-153．8．J．1999113129-131．9．J．1988811-4．10．J．2008241-43．11．SB900J．199934188-92．12．J．2008384551-556．13．036201112J．200222449-51．14IshiharaKHayasakaKYamazakiN．CurrentstatusofmelanomatreatmentwithinterferoncytokinesandotherbiologicalresponsemodifiersinJapanJ．JInvestDermol198992326-328．15SekineKWatanabSEOhtaJetal．InductionandactivationoftumoricidalcellsinvivoandinvitrobythebacterialcellwallofbifidobacteriuminfantisJ．BifidobacteriaMicroflora19941365-72．16SunJShiYHLeGWetal．Distinctimmunere-sponseinducedbypeptidoglycanderivedfromLacto-bacillusspJ．WorldJGastroen-tero1200511406330-63378．17．J．2005235500-502．18．Lb．casei．ZhangJ．J．2008283353-356．19．J．200839396-99．ResearchonPeptidoglycanofBacterialCellWallLIUFangYANGYue-huanSchoolofBiotechnologyEngineeringSichuanUniversityofScience＆EngineeringZigong643000ChinaAbstractPeptidoglycanisanuniquecompositionoftherealbacterialcellwallbecauseitplaysamajorroleinthephysiologicalfunctionsofthebacterialcellwallandbecomesthemainsubjectofresearchonthebacterialcellwallatpres-ent．Thechemicalcompositionstructuralfeatureclassificationseparationandpurificationbiosynthesisbiologicalfunc-tionapplicationprospectofpeptidoglycanofthebacterialcellwallandsoonareintroduced．Keywordspeptidoglycanseparationandpurificationbiosynthesisbiologicalfunction136246