西瓜重组自交系群体的AFLP分子图谱构建

©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(1):5358ActaHorticulturaeSinica:2003-04-24;:2003-09-26:(30070527);863(2001AA241174);948(201024);(5991017)3,:010-51503035,Email:[e-mail]12311122(1,410128;2,100089):(Citrulluslanatusvar.lanatus)97103(Citrulluslanatusvar.citroides)PI296341F2S7,AFLP,15017,124012cM,813cMQTL:;AFLP;RIL;:S651:A:05132353X(2004)0120053206ConstructionofAFLPMolecularGeneticMapforRILPopulationofWatermelonYiKe1,XuYong23,LuXiangyang1,XiaoLangtao1,XuXiangli,GongGuoyi2,andZhangHaiying2(1DepartmentofBiotechnologyofHunanAgricultureUniversity,Changsha410128,China;2NationalEngineeringResearchCenterforVegetables,Beijing100089,China)Abstract:Arecombinantinbredlinesof117F2S7progeniesderivedfromthecross97103(whichisacultivarwithhighertotalsolublesolidsconcentration,thinrind,susceptibletoFusariumwiltdisease)PI296341(whichisanAfricanwildgermplasmwithlowertotalsolublesolidsconcentration,thickrind,resistanttoFusariumwiltdisease)wasusedtoconstructamapcontaining150AFLPmarkers.Thismapconsistsof17linkagegroups,cov2ering124012cMwithanaveragedistanceof813cMbetweenlocus.Itwillcontributetoconstructionofahigh2den2sitymapofwatermelon,QTLanalysisofimportantagronomictraitsandmap2basedgeneclone.Keywords:Watermelon;AFLPmarker;Recombinantinbredlines;Linkagemap1,2,3,,,48,F2BC1,,,QTL,,,,(RILs),RFLPRAPDAFLPSSR,AFLPRAPDRFLP,997103PI296341F25,AFLP©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(Thunb.)Mansfeldvar.citroides(ClemsonB.B.Rhodes,PI296341,,,,,,3),Citrulluslanatus(Thunb.)Mansfeldvar.lanatus97103(,)97103PI296341,117F2S7DNA:CTABDNA,Murry10AFLP:AFLPAFLPAFLPTMGIBCOBRL,rTaqEdNTP()(Takara,Dalian)AFLPSequi2GT(Bio2Rad,America),AFLP:AFLPAFLP,5L,:1buffer,1125mmol/LMg2+,0125mmol/LdNTP,014mol/LIRD-Eco-,15ngMse-,015L20,0125UrTaqE6%4200202GNENGlobalIR2DNA(2dye,Li2CoR,America)(50700bp,IRdye700,Li2CoR,America):AFLPA,B,-PI296341A,97103B,-,Mapmaker/Expversion310,LOD5100130,Group,order,rippleKosambi,Map,(threepoint)Mapchartversion210221111EcoRI/MseI,DNA(1),115113%,287,2611,40650bp(1)AFLP,,197103PI296341RITable1Infrareddye2labeledprimerstestedinRIpopulationfrom97103PI296341PrimersTotalbandsPolymorphicbandsPrimersTotalbandsPolymorphicbandsE2AAC/M2CAA7541E2AAG/M2CTA6125E2AAC/M2CAT4625E2AAG/M2CTC4622E2AAC/M2CTC3924E2AAG/M2CTT5426E2AAC/M2CTT5829E2ACA/M2CAC3523E2AAG/M2CAC4325E2ACC/M2CAA4823E2AAG/M2CAT5424Total5592874531©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:PI296341;2:97103;3:F1;442:RILFig.1AFLPprofilesgeneratedbyE2AAGM2CACinRIpopulationM:100bpDNALadder;1:PI296341;2:97103;3:F1;4-42:partsofRILpopulation212AFLPRIL221AFLP,67,30%(0105P0101),5797103,85%;10PI296341,15%97103,19,21(ACAA141,ACAA366,CAAC76,CAAC84,CAAC89,CAAC200,CCAA94,ACAT145,ACAT188,ACTC78,ACTC101,ACTT250,ACTT438,ACTT472,AGAC100,AGAC126,AGAT97,AGAT112,AGTA142,AGTA163,AGTC235)1,5(ACAA91,ACAA120,,ACAA181,ACAA123,AGAC63)2,1(ACAT96)3,3(AGTT81,ACTC97,ACTT279)6,1(ACAA84)7,1(ACTC107)8,1(AGTA391)9,1(AGTA80)12,1(ACAA160)14,3(ACAA134,ACAT142,AGTT133)17PI29634110,3,1(CCAA280)1,3(AGAT142,AGAC354,ACAT159)551:AFLP©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(AGTT131,ACTC170,AGAC270)311213221,150AFLP,96PI296341,549710317,124012cM,813cM(2),AFLP,LG1LG2,LG1,44AFLP,37217cM,817cM,2(97103PI296341)RILAFLPFig.2AAFLPmolecularmapforRILsofwatermelon6531©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(RIL)DH,,,,RIL,RILQTLF25,AFLP,413105kb,124012cM,,813cM,F2(r=0105),,312AFLP(),AFLP12,1350100,,AFLP287,5113%,RAPDSSRISSR,AFLP,,,,,AFLP,HuangJ.C.,14AFLPAFLP,,,AFLP,,,AFLP,,,,37217cM,F25,48,Levi20719cM22516cM6,7,,,313,15QTL,41%23%,1636%AFLP,(hitchhiking),17,Xu,,751:AFLP©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(0105P0101),AFLP,97103,85%(P0105),97103,PI296341,,(97103)11,RILF2,,RIL,(near2isogeniclines,NIL),,:1LindhoutP.Perspectivesofmolecularmarkerassistedbreedingforearlinessintomato.Euphytica,1994,79:2792862MartinGB,BrommonschenkelSH.Map2basedcloningofaproteinkinasegeneconferringdiseaseresistanceintomato.Science,1993,262:143214363,..,2001,23(5):4985024HashizumeT,SkimamotoI,HarashimaY,etal.ConstructionofalinkagemapforwatermelonCitrullus(Thunb)Matsum&NakaiusingRAPD.Euphytica,1996,90:2652735,,,.QTL.,2000,27(10):9029106LeviAmnon,ClaudeEThomas,ZhangXP,etal.AgeneticlingkagemapforwatermelomnbasedonrandomlyamplifiedpolymorphicDNAmark2ers.J.Amer.Soc.Hort.Sci.,2001,126(6):7307377LeviAmnon,ThomasClaudeE,JoobeurT,etal.Ageneticlinkagemapforwatermelonderivedfromatestcrosspopulation:(Citrulluslanatusvar.citroidesC.lanatusvar.lanatusCitrulluscolocynthis).Ther.Appl.Genet,2002,(10):1198HashizumeT,ShimamotoI,HiraiM.ConstructionofalinkagemapandQTLanalysisofhorticulturaltraitsforwatermelonCitrulluslanatus(Thunb.)Matsum&NakaiusingRAPD,RFLPandISSRmarker