高选择性重组菌在含Hg

44()Vol.44Sup.20056JournalofXiamenUniversity(NaturalScience)Jun.2005Hg2+Hg2+Hg2+:2004212230:,(D0310004):(1977-),,.3:0592-2183088,3,,,,,(,361005):,E.coliJM109(MerTMerPMT,M1).Hg2+;,.:E.coli;;;:X131.2:A:043820479(2005)Sup20098204[1].,,,,[2].[3,4].,.,..11.11):E.coliJM109(M1),(MerPMerT)GST2MT,Kanr()Am2pr()[5].Hg2+,Hg2+Hg2+.2)LB:10g/L,5g/L,NaCl10g/L;0.5LB:5g/L,2.5g/L,NaCl2.5g/L.3):Kan(),Amp(),IPTG(22D2).4):ZYG2,UV310,,DHL2A.1.260mg/LKan100mg/LAmpLB,37.60mg/LKan100mg/LAmp0.5LB,IPTG1.0mol/L,.,,.UV310600nm;1OD6000.35g/L[6].ZYG2.M1Hg2+Hg2+,Hg2+1mg/L,.,350mL0.5LB,,2mg/LHg2+0.5LB,FF,.1Hg2+M1Fig.1EffectofHg2+onSG&Bin0.5LB22.1M1Hg2+3Ca2+M1Hg2+Fig.3EffectofCa2+onSG&Bin0.5LB0.5LB,M1.1,1mg/LHg2+M1,OD6001.90,Hg2+90%.Hg2+2mg/L,M1,OD6000.73,Hg2+,85%.Hg2+4mg/L,M1,OD6000.25,Hg2+37%,,(),M1Hg2+11mg/LLB,OD6001.2,.[7].M1Hg2+Hg2+,M1Hg2+1mg/2Na+M1Fig.2EffectofNa+onSG&Bin0.5LBLHg2+1.5mgHg2+/gdrycell4mg/LHg2+25mgHg2+/gdrycell.,/,[8,9].4EDTAM1Hg2+Fig.4EffectofEDTAonSG&Bin0.5LB2.2(Na+Ca2+Mg2+)M1Hg2+2,Na+M1,0.04mol/LNa+M1.Na+M1.Na+0.90mol/L,M1Na+,OD6000.40,Hg2+80%.M1Hg2+Na+.3,Ca2+M1,0.1mol/LCa2+M1,Hg2+Ca2+,95%.Ca2+M1,Hg2+.Mg2+M1Ca2+().Ca2+Mg2+Na+,Ca2+Mg2+M1Na+.2.3M1Hg2+99:Hg2+Hg2+Hg2+5M1Hg2+Fig.5EffectofcitrateonSG&Bin0.5LB4,1.4mmol/LEDTAM1,OD6000.13,Hg2+65%,Hg2+.EDTAM1Hg2+,M1EDTA,Hg2+.,M1Hg2+EDTA[4],EDTAM1Hg2+[10].5,M1.17mmol/L7(10mg/L)Hg2+Fig.7Effectsofco2ionswithinitialconcentrationof10mg/LontheHg2+bioaccumulationin0.5LBM1.34mmol/L54mmol/L,M1.M1Hg2+,Hg2+90%.EDTAM1,EDTAM1.EDTAM1,EDTAM1M1,M1;6(10mg/L)M1Fig.6Effectsofco2ionswithinitialconcentrationof10mg/LonthegrowthofM1in0.5LBM1.EDTA,M1;,.8D=0.20h-1,Cin=2mg/LFig.8CSTRoperationunderD=0.20h-1,Cin=2mg/L2.4M1Hg2+6,M1,.Cd2+Ni2+,M1OD6000.251.55.M1:Cd2+Zn2+Cu2+Pb2+Ni2+.7,Cd2+Cu2+,M1Hg2+,Hg2+90%.Cd2+Cu2+Hg2+,,Hg2+.4a4b,Cu2+Zn2+M1,Hg2+,.2.58D=0.20h-1,Cin=2001()2005mg/L,CoutOD600.,12.5h,M1,Hg2+,5.5h,Hg2+,90%Hg2+.,,.,0.92mgHg2+/gdrycell.M1.3,M1Hg2+.Hg2+M1,.Na+M1,Hg2+Na+.Ca2+Mg2+M1,M1.EDTAM1,M1Hg2+;M1.M1,;Cd2+Cu2+,M1Hg2+,Hg2+90%.M1.:[1]GoyalN,JainSC,BanerjeeUC.Comparativestudiesonthemicrobialadsorptionofheavymetals[J].Adv.Envi2ron.Res.,2003,7:311-319.[2],,,.[J].,1998,14(2):180-187.[3]DengX,LiQB,LuYH,etal.BiaccumulationofnickelfromaqueoussolutionsbygeneticallyengineeredEsche2richiacoli[J].Wat.Res.,2003,37:2505-2511.[4]ChenShaolin,DavidBWilson.Geneticengineeringofbac2teriaandtheirpotentialforHg2+bioremediation[J].Bio2degradation,1997,8:97-103.[5]ChenShaolin,DavidBWilson.Constructionandcharac2terizationofEscherichiacoligeneticallyengineeredforbioremediationofHg2+contaminatedenvironments[J].Appl.Environ.Microbiol.1997,63(6):2442-2445.[6]ChenShaolin,EunkiKim,MichaelLShuler,etal.Hg2+removalbygeneticallyengineeredEscherichiacoliinahollowfiberbioreactor[J].Biotechnol.Prog.,1998,14:667-671.[7]OkinoS,IwasakiK,YagiO,etal.Removalofmercuricchloridebyageneticallyengineeredmercury2volatilizingbacteriumPseudomonasputidaPpY101/pSR134[J].Bull.Environ.Contam.Toxicol.,2002,68:712-719.[8]ChandraSekharK,SubramanianS,ModakJM,etal.Re2movalofmetalionsusinganindustrialbiomasswithref2erencetoenvironmentalcontrol[J].Int.J.Miner.Process,1998,53:107-120.[9]HandanUcan,YKemalBayhan,YusufKaya,etal.Bio2sorptionofchromium(VI)fromaqueoussolutionbyconebiomassofpinussylvestris[J].Bioresour.Technol.,2002,85:155-158.[10]DengX,WilsonDB.BioaccumulationofmercuryfromwastewaterbygeneticallyengineeredEscherichiacoli[J].Appl.Microbiol.Biotechnol.,2001,56:276-279.SimultaneousGrowthandBioaccumulationofMercuricIonbyGeneticallyEngineeredE.coliandItsContinuousTreatmentZHENGYang2chun,DENGXu3,LIQing2biao,LUYing2hua,HENing,SUNDao2hua,PENGYa2juan(DepartmentofChemicalandBiochemicalEngineering,XiamenUniversity,Xiamen361005,China)Abstract:GeneticallyengineeredE.coliJM109,namelyM1,whichexpressedbothHg2+transportsystemandmetallothionein,wastestedforitscapabilityofsimultaneousgrowthandbioaccumulationofHg2+(SG&B)underlownutritionalcircumstances.Theinflu2entialfactorsofambientconditions,e.g.initialconcentrationsofmercuricion,ionicstrength,thepresenceofmetalchelatorsandoth2ercoexistingmetalionswereinvestigated.Hg2+bioaccumulationbehaviorofM1wasprovedtobewellcoupledwithitsgrowthofM1.Furthermore,continuoustreatmentofHg2+contaminatedorganicwastewaterwasrealizedviacontinuousstirred2tankbioreactor.Keywords:geneticallyengineeredE.coli;mercuricion;wastewater;bioaccumulation101:Hg2+Hg2+Hg2+