阿莫西林和硫酸粘菌素对猪鸡大肠杆菌和沙门氏菌的体外联合抗菌作用

,,,,(,,430023)[]2008-11-19[]A[]1002-1280(2009)05-0030-03[]S859.79+6[](MIC),MIC(FIC),1478.6%,21.4%,,[];;;;;:(1982-),,,,:E-mail:[e-mail]ctivityofAmoxicillinCombinationwithColistinSulfateagainstEscherichiacoilandSalmonellafromPigandChickenMAHong-wei,WUTao,XIAOFei,GAOHai-pen,QIUYin-sheng(FeedScienceDepartmentofWuhanPolytechnicUniversity,HubeiFeedEngineeringTechnologyResearchCenter,Wuhan,430023;China)Abstract:TheinhibitoryeffectsofamoxicillincombinationwithcolistinsulfateonEscherichia.coilandSalmonellaofpigandchickenwereinvestigated.Theminimalinhibitoryconcentration(MIC)ofamoxicillinandcolistinsulfateinvitrowasdetectedbytwo-folddilutionrespectively.TheMICofcombinationamoxicillinwithcolistinsulfatewasdetectedbycheckerboardmethod,andthefractionalinhibitoryconcentration(FIC)wascalculated.Ingeneral,synergyandadditiveeffectrateofcombinationofamoxicillinwithcolistinsulfatewas78.6%invitroagainst14strainspathogenicbacterial,andtherewasnoantagonismaftercombinationoftwodrugs.Keywords:amoxicillin;colistinsulfate;Escherichiacoil;Salmonella;MIC;combineuseofmedicine,,[1-2],,,,,,032009,43(5):3032/,11.1,587598;,1006916,OXOIDLTD,PB10025,,1.2,308612533,9517%;,0608166,20946/mg,1.35,3;1,1,O54O78C79-13A-72,1.4,,,SXP-250B-G,YG-1,,,AE-20022.1LBJ.[3]2.2102.4mg(),100mLpH6.0,1024g/mL6.4mg()100mL,64g/mL,6.4g/mL,0.64g/mL2mLEP,-202.312,58mLLB,3756h,1012h10-1:10-110-210-310-410-510-610-710-8;10-610-710-8100LLB,,,(),3;937,12h,24h,,1mL,2.4MICNCCLS12896V,1250L112,1105CFU/mL37,810h,1824h,,MIC[4]2.5MIC,6,MIC2,11/21/41/81/16,961105CFU/mL,37,810h,24h[5](FIC)FIC=(MIC/MIC)+(MIC/MIC):FIC0.5;0.5FIC1;lFIC2;FIC233.111MICFICMIC/(gmL)MIC/(gmL)FIC132640.0050.020.75216320.0010.0021.0038640.010.160.1844160.0050.020.558320.020.021.2511281280.40.42.002642560.20.40.7534160.10.20.75O781282560.050.11.00A272162560.0070.0150.56MIC16256g/mL,MIC5032g/mL;MIC16512g/mL,[6-7]132009,43(5):3032/,MIC0.00250.16g/mL,MIC500.02g/mL;MIC0.01560.8g/mL,[8-9]3.212,32MICFICMIC()/(gmL)MIC()/(gmL)FICAPEC32640.010.021.0064640.40.81.50O548640.01250.0250.62C79-131285120.0310.0621.003/%/%/%/%FIC825.062.512.500.786066.733.301.091414.364.321.400.944,(),,,()[10],,,,,,,,,,,,,14,78.6%,21.4%FIC0.781.09,FIC0.94,I,:[1],,,.[J].,2007,43(10):31-33.[2],,,.[J].,2008,44(1):18-20.[3]()J,DW.()[M]..:,2002:1595.[4]NationalCommitteeforClinicalLaboratoryStandards.Perform2anceStandardsforAntimicrobialSusceptibilityTesting[S].Pennsylvania:NCCLS,2001:1-123.[5],.[M].:,2002:47-56.[6],,,.[J].,2005,27(2):10-13.[7],.(Colistinsulfate)[J].,2003,11(23):604-606.[8],.[J].,2006,40(9):34-36.[9],,,.B[J].,2007,31(3):7.[10].[M].,2003:266-267.232009,43(5):3032/,