平衡透析法用于有机小分子和蛋白质相互作用研究进展-杨海艳

第36卷第4期2008年7月河南师范大学学报(自然科学版)JournalofHenanNormalUniversity(NaturalScience)Vol.36No.4July.2008:1000-2367(2008)04-0093-05杨海艳,王公轲,陈得军,卢秀敏,卢雁(,453007):,...,,..:;;;:O658.9:A,[1-2],.,[1],Scatchard.,.,;,..1.,..,,.,(),.,.,,,.22.1ScatchardScatchardCl-(HSA)Scatchard[3],.:,(),,.Scatchard:n/[L]=KN-Kn,(1)n,[L],K,N:2007-09-29:(20673034);(20060476001):(1953-),,,,,.,nn/[L],NK.Congdon[4]CBBG-250,Scatchard,:1/[L]=KN/n-K,(2)1/[L]=(1/KN)(1/[L])+1/N.(3)Hilln/(n-1),:(n-1)/n=-(1/NK)(1/[L])+(N-1)/N.(4)Congdon,,.,.(3)(4),KN(2).1~2,(4);4~5,,(2).2.2Taira[5],.Scatchard,;,,.,.n=ns+nns,(5)ns,ns=NKs[L]/(1+Ks[L]);nns,nns=Kns[L](S-NS).TairaScatchard(),,,.,,.2.3Plsavento[6]Scatchard,.(BSA),.Plsavento:,,Kd=n/CF,n/CF.,PlsaventoTazoR(123,6),.3:(1);(2)().,.3.11946,Scatchard[7-9],.Klotz[10,11],1946,,,.,pH.1947,Klotz[12]pH.(),,.,,.pH,,pH9.0,.pH9.0,pH,pH=9.0,pH9.0,,.pH94河南师范大学学报(自然科学版)2008年,,,.,pH=5~13,,pH4.5,H+.KraghHansen[13].,;,;,pH,,.pH,[14].pH,,,BSA.,.,,,:[13,14][15][16].M.N.JonesP.Manley[17]25,,.,[18],Gv.=RTv0vdln[S],(6)Wyman,R,T,v,[S].JuliaCordoba[19]25,pH=6.4,0.132mol/L.,,[20]HABA[2-(4-hydroxyphenazo)benzoicacid][21].,.1996,,[22]().()pH=2.0,3().30.11.11061053.210436.58.41051068.2104391.01061074.51043.,,;,,,;,.[23]K(ACBK)BSA,.:pH=9.91ACBK,Pesavento,D,ACBK.3.2.,,.1997,PetersenCE.[24]-214HSA.(HSA)(2A),,2A,.PabloTaboada[25]25HSA(penicillin,nafcillin,cloxacillin,dicloxacillin,andflucoxacillin)pH=7.4.,,HSA1200(di95第4期杨海艳等:平衡透析法用于有机小分子和蛋白质相互作用研究进展cloxacillin)3000(nafcillin).Nafcilincloxacillin,,.2001,[26]pH=4.47,(CCA)(BSA).,CCABSA,Pesavento,CCABSA.[27],HSABSA.pH=7.43HSABSA,HSABSA.Scatchard,HSABSA.Bjerrum,HSABSA.2003,,[28]2-(8--5--7-)-1,8--3,6-BSA,8Q5SACBSA,.298K,35~40,6.1105Lmol-1.8Q5SACBSA,pH=3.34,0.21~46.90mgL-1.[29]ZnPcS2P2(,)BSA.Hill,ZnPcS2P2BSA,.,.,,.4,..,,.,.,,,.[1],,,.[J].,1999,21(1):17-21.[2],.[J].,2001,29(2):232-241.[3]ScatchardG,ScheinbergIH,ArmstrongSH.Physicalchemistryofproteinsolutions,IV.Thecombinationofhumanserumalbuminwithchlorideion[J].JAmChemSoc,1950,72:535.[4]CongdonRW,MuchGW,SplittgerberAG.ThebindinginteractionofCoomassiebluewithproteins[J].AnalBiochim,1993,213:407.[5]TairaZ,TeradaH.Specificandnonspecificligandbindingtoserumalbumin[J].BiochemPharmacol,1985,34:1999-2005.[6]PlesaventoM,ProfumoA.InteractionofSerumAlbuminwithASulphonatedAzoDyeinAcidicSolution[J].Talanta,1991,38:1099-1106.[7]ScatchardG.Physicalchemistryofproteinsolutions.I.Derivationoftheequationsfortheosmoticpressure[J].JAmChemSoc,1946,68(11):2315-2319.[8]ScatchardG,BatcheladerAC,BrownA.Preparationandpropertiesofserumandplasmaproteins.VI.Osmoticequilibriainsolutionsofserumalbuminandsodiumchloride[J].JAmChemSoc,1946,68(11):2320-2329.[9]ScatchardG,BatcheladerAC,BrownA,etal.PreparationandPropertiesofSerumandPlasmaProteins.VII.OsmoticEquilibriainConcentratedSolutionsofSerumAlbumin[J].JAmChemSoc,1946,68(12):2610-2612.[10]KlotzIM,WalkerFM,PivanRB.Thebindingoforganicionsbyproteins[J].JAmChemSoc,1946,68(8):1486-1490.96河南师范大学学报(自然科学版)2008年[11]KlotzI.M.SpectrophotometricInvestigationsoftheInteractionsofProteinswithOrganicAnions[J].JAmChemSoc,1946,68:2299.[12]KlotzIM,WalkerFM.TheBindingofOrganicIonsbyProteins.ChargeandpHEffects[J].JAmChemSoc,1947,69:1609-1612.[13]KraghHansenU,MollerJV.Proteinbindingofsmallmolecules.IV.Relationbetweenbindingofphenolsulfophthaleindyesandotherligandswithahighaffinityforhumanserumalbumin[J].BiochimBiophysActa,1974,336:30.[14]KraghHansenU,MollerJV.Proteinbindingofsmallmolecules.I.EffectofpHandinorganicsaltsonthecombinationofphenolredwithproteins[J].BoichimBiophysActa,1973,295:438.[15]JonasA,WeberG.Presenceofarginineresiduesatthestrong,hydrophobicanionbindingsitesofbovineserumalbumin[J].Biochemistry,1971,10:1335.[16]ComptonSJ,JonesCG.MechanismofdyeresponseandinterferenceintheBradfordproteinassay[J].AnalBiochem,1985,151:369.[17]JonesMN,ManleyP.Bindingofnalkylsulphatestolysozymeinaqueoussolution[J].JChemSoc,FaradayTrans.1979,75:1736.[18]JonesMN,ChapmanD.Micelles,MonolayersandBiomembranes[M].NewYork:WileyLiss,1995.[19]CordobaJ,ReboirasMD,JonesMN.InteractionofnoctylbDglucopyranosidewithglobularproteinsinaqueoussolution[J].InternationalJournalofBiologicalMacromoleules,1988,10(5):270-276.[20]BrackenJS,KlotzIM.Asimplemethodfortherapiddetectionofserumalbumin[J].AmericanJournalofClinicalPathology,1953,23:1055-1058.[21]PorterEG,WatersWJ.Arapidmicromethodformeasuringthereservealbuminbindingcapacityinserumfromnewborninfantswithhyperbilirubinemia[J].JLabClinMed,1966,67:660-668.[22],,.III[J].,1996,24(1):10-14.[23],.K[J].(),2003,21(2):121-125.[24]PetersenCE,HaCE,HarohalliK,etal.Mutagenesisstudiesofthyroxinebindingtohumanserumalbumindefineanimportantstructuralcharacteristicofsubdomain2A[J].Biochemistry,1997,36:7012-7017.[25]PabloTaboada,VictorMosquera,JuanM.Ruso,FelixSarmiento.Interactionbetweenpenicillinsandhumanserumalbumin:Athermodynamicstudyofmicellarlikeclustersonaprotein[J].Langmuir,2000,16:934-938.[26],,.[J].(),2001,1:88-92.[27],,.HSABSA[J].,2001,31(6):530-535.[28],.2(857)1,83,6(BSA)[J].,2003,31(8):976-980.[29],,,.[J].,2003,31(10):1159-1163.ResearchDevelo