人肠道微生态系统的研究进展

().(:16SrRNA).30500[1],.,.,,.,,.,,,,,.[2]..,.,,40%80%[3],.,Segmenetedfilamentousactria;,,[4]..1.[1,5],,99%,90%..,,,103CFU/ml.,105108CFU/ml,(102105CFU/ml).,,1091012CFU/ml,:(1081011CFU/ml)(104109CFU/ml),.:.,,,;,,,,,,;.,,,,,,..2.[6],,..(1)pH:(lymphokines).:,.:(compartmentalisation).88ZiranZazhiVol.26No.2©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(2):.:().(3):,(Eh)pH./:EhpH;().(4):.3.[1,7],,,.,.,..,,,,.,,.,.[8,9],.,,,,.1.,:,.NH3,,.,,,,.2.,,,.,(90%),,pHEh,.,,,.,,,.,H2O2.3..,,4/5.,.,,IgA.IgG1/10.,,.,.4.,.,,,.,,.,,(),,..98262©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:1.16SrRNA(polymerasechainreaction,PCR)[10]16SrRNA,.,PCRDNA,.,.(consensusse2quence).:,.EMBLDDBJPDBGenBankRibosomalDatabaseliberary()DNA.100%,.Frothingham[11]7816SrRNA,716SrRNA94%99%.(B.infantis)(Actinomycesbovis,Arhtro2baterglobiformis,Mycobacteriumbovis,Saccharothrixaustr2lienseandStrptomycescoelicolor),.Leblond[12]4516SrRNA,GenBank/EMBL16SrRNA,16SrRNA.[13]16SrRNAV10,615PCR,.2.(denaturinggradientgelelec-trophoresis,DGGE)rRNA.:530-50bpGC(GC-clamp),16SrRNAPCR,GC,DNA;DNA,,DNA[14].,.DGGE.,.Favier[15]DGGE16SrRNA,DGGE,.,DGGE,,,.Temmerman[16]DGGE10,,;106,,DGGE,5.3.(pulsed-fieldgelelectrophoresis,PFGE)[5]DNA.(8bp6bp)(5-50),,DNA.,PFGE,.PFGE,,,.RousselY[17],PFGE9LactobacillusacidophilusLactobacillusacidophilus,PFGELact.aci2dophilusLact.Gasseri,1.852.02Mb.McCartney(1996)Kimura(1997).PFGE,23,,.09ZiranZazhiVol.26No.2©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(restrictionfragmentlengthpolymorphism,RFLP)[18]16SrRNA,16SrRNA,,,,..DNA/,.RFLP,,.Mangin[19]21,RFLP,218.RFLPDNA,RFLP.5.(randomamplifiedpolymorphicDNA)10-12,DNA,DNA,PCR,,DNA,DNA.DuPlessis[20]Lactobacil2lusacidophilusLb.crispatusLb.amylovorttsLb.galli2narumLb.gasseriLb.jonhsonii.,,.,,.6.(fluorescentinsituhybridization,FISH),,.Langendijk[21],16SrRNA,.Harmsen[22]716SrRNA6(Phascolarcto2bacterium,Veillonella,Eubacteriumhallii,Lachnospira,Eu2bacteriumcylindroids,Ruminococcus),90%.,.,,.,,,.(2003117),,200240,,2002401,.,2002;19(2):882902,.,1999;29(1):342433GuarnerF.,MalageladaJ.R.TheLancet,2003;360:51225194,.,2003;25(6):322345..:,2002:452656HolzapfelW.H.,etal.InternationalJournalofFoodMicrobiology,1998;41:8521017..:,2001:11521168..:,19969,.,1999;13(4):3924210,.,2001;22(5):265226811FrothinghamF.,AduncarJ.,WilsonK.H.MicrobEcolHealthDis,1993;6:2312LeblondB.,etal.IntJSystBacteriol,1996;46:10213,.,2001;21(5):578257914MuyzerG.,SmallaK.AntoniVanLeeuwenhoek,1998;73:127214115FavierC.F.,etal.Appl.Environ.Microbiol.2002;68(1):219222616TemmermanR.,etal.Appl.Environ.Microbiol.2003;69(1):220222617RousselY.,ColminC.JApplBacteriol,1993;74(5):549255618,..:,2002:2722919ManginL.,etal.Appl.Environ.Microbiol.1994;60:145120DuPlessisE.M.,DicksL.M.T.CurrMicrobiol.,1995;31:114211821LangendijkP.S.,etal.Appl.Environ.Microbiol.,1995;61(8):30692307522HarmsenH.J.M.,etal.Appl.Environ.Microbiol.,2002;68(6):298222990ProgressintheStudyofHumanIntestinalMicroe2cosystemJinHong-zhi,LiKun-baoGraduateStudent,SchoolofLifeScienceTechnology,ShanghaiJiaotongUniversity,Shanghai200240Professor,SchoolofLifeScienceTechnology,ShanghaiJiaotongUniversi2ty,Shanghai200240Keywordsmicroecosystem,intestinalflora,molecularmethods19262©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.