用清除有机自由基DPPH法评价植物抗氧化能力

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,510650)1,12(DPPH).DPPH.DPPH.DPPH,..1,12(DPPH),,,Q946.,,.(thiocyanate)[1](TBA)[2]ORAC(automatedoxygenradicalabsorbancecapacityassay)[3].,,.1,12(1,12Diphenyl222picryl2hydrazyl,DPPH),DPPH..,DPPH[4,5],.DPPH,.1111(BeckmanDU27),(1,12diphenyl222picrylhydrazyl,DPPH),,(TBA),,(AsA)(QCT)(GSH)(BHT),Sigma,2(2TP)Merck.1,2223,52(Tiron),,.112(Castanopsisfissa)(Psycotriarubra).(100%)36%16%.012g50%,(5000g,15min),10ml.113(DPPH)LarrauriYokozawa[4,5].DPPH,A525.2ml,DPPH,50%120mol/L.10,011ml119mlDPPH.20min.DPPH=12[(A-B)/A0]100%,A0DPPH(119mlDPPH+011ml50%),ADPPH,B(011ml+119ml3(KZ9522J12105).Tel:[phone],E2mail:[e-mail]:1999211220,:2000204202856Prog.Biochem.Biophys.2000;27(6)©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.).:[(DPPH)/(g)]DPPH.1140138mmol/L0133mmol/LH2O2,5h,,(TBA)(MDA)[6].MDA.115[7],O2,AsAO2,480nm().2211DPPHDPPH330nm525nm,(AsA)(QCT)(1),525nm,525nmDPPH,Larrauri[4]DPPH517nm.1DPPH212DPPH(GIP)(QCT)(GSH)2(2TP),BHT,Tiron.2DPPH(2),rBHT018977(2b),019670019935.,DPPH.,DPPH.2DPPH(A525)(a):QCT,y1=-010872x+110543,r=019904;:GIA,y2=-012485x+110843,r=019935;(b):BHT,y3=-010245x+019008,r=018977;:GSH,y4=-010149x+110715,r=019918;(C):2TP,y5=-010233x+018558,r=019788;:Tiron,y6=-010212x+017586,r=019670.213DPPH1DPPH50%(IC50)DPPH,,,GSH2TPBHTTiron.Cao[8]ORACASA2TPGSH.9562000;27(6)Prog.Biochem.Biophys.©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(DPPH50%)/gc/molL-1DPPH/gg-1GIP2111612324122QCT516791317170AsA8141261535107BHT13190351132183GSH341366211211302TP19150221631119Tiron16180381121187214DPPH.3DPPH(AsA).AsADPPH-010638,019986(3b),AsA-010011,019775(3a).DPPHAsA,.,,(O2),DPPHDPPH.3(a)DPPH(b)(a)y1=-010011x+011619,r=019775;(b)y2=-010638x+110962,r=019986.215DPPH.DPPH(4),.,.,,.DPPH.4DPPH(a)(b):;:.,DPPH(066Prog.Biochem.Biophys.2000;27(6)©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.)(,)()(DPPH,DPPH,).1OsawaT,NamikiMA.NoveltypeofantioxidantisolatedfromleafwaxofEucalyptusleaves.AgricBiolChem,1981,45(3):7357392OttolenghiA.Interactionofascorbicacidandmitochondriallipides.ArchBiochemBiophys,1959,79:3553633CaoG,AlessioHM,CulterRG.Oxygen2radicalabsorbancecapacityassayforantioxidants.FreeRadicalBiolMed,1993,14(3):3033114LarrauriJA,Sanchez2MorenoC,Saura2CalixtoF.Effectoftemperatureonthefreeradicalscavengingcapacityofextractsfromredandwhitegrapepomacepeels.JAgricFoodChem,1998,46(7):269426975YokozawaT,DongE,NatagawaT,etal.Invitroandinvivostudiesontheradical2scavengingactivityoftea.JAgricFoodChem,1998,46(6):214321506,,,.,,1984,26(6):605615LinZF,LiSS,LinGZ,etal.ActaBotSin,1984,26(6):6056157,,.,,1989,16(3):209WongYK,HuangS,WongLY.ProgBiochemBiophys,1989,16(3):2098CaoG,SoficE,PriorRL.Antioxidantcapacityofteaandcommonvegetables.JAgricFoodChem,1996,44(11):34263431DetectionofAntioxidativeCapacityinPlantsbyScavengingOrganicFreeRadicalDPPH.PENGChang2Lian,CHENShao2Wei,LINZhi2Fang,LINGui2Zhu(SouthChinaInstituteofBotany,TheChineseAcademyofSciences,Guangzhou510650,China).AbstractAverysignificantlinearrelationshipwasfoundbetweenthecapacityofscavengingDPPHfreeradicalandconcentrationsofsixantioxidants(r=0189801994)determinedbyspectrophotometry.TherewasanobviousdifferenceinthecapacityofscavengingDPPHfreeradicalamongdifferentantioxidants.BothscavengingDPPHandinhibitingtheoxidationofadrenalinwerecloselyrelatedwiththeconcentrationofascorbicacid.ThechangeofDPPHlevelsismoresensitivethanthatofadrenalininthepresenceofascorbate.TheantioxidativeabilityinleavesextractsoftwowoodyplantsgrownunderdifferentlightintensitieswasmeasuredbyeitherscavengingDPPHorinhibitingtheoxidationoflinoleicacid.Thesameconclusionwasdrawnthroughthesetwoassays.ItissuggestedthatscavengingDPPHfreeradicalisarapid,simple,sensitiveandpracticalassayfortheevaluationofantioxidativecapacityinplants.Keywords1,12diphenyl222picrylhydrazyl(DPPH),plant,antioxidativecapacity,freeradical(FAOBMB)1021241992Fischer,1997Boyer,1975Baltimore,21,(IUBMB)199910,21,,,,,,:15:100101:010264889892:[e-mail]1662000;27(6)Prog.Biochem.Biophys.