自动定氮仪测定水产品中的挥发性盐基氮

16200512SouthChinaFisheriesScienceVol11,No16Dec.,2005:2005207225;:2005209202:(1954-),,,E2mail:cpjcx@1261com,,(,510300):(Tilapianiloticus)(Decapterusmaruadsi)(Litopenaeusvannamei)(Crassostrearivularis)4(TVBN):016molL-12min4TVBN,98142%100118%,1140%GB/T5009144,,:;;;:S98511+1;O657:A:1673-2227-(2005)06-0050-05DeterminationoftotalvolatilebasicnitrogeninaquaticproductsbynitrogenautoanalyzerCHENPei2ji,LILiu2dong,HUANGKe(SouthChinaSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Guangzhou510300,China)Abstract:FourspeciesofaquaticproductsasTilapianiloticus,Decapterusmaruadsi,LitopenaeusvannameiandCrassostrearivulariswerepretreatedbyperchloricacid(HClO4)solutiontoextractthetotalvolatilebasicnitrogen(TVBN),whichwasdeterminedbynitrogenautoanalyzer1TheinfluenceofdifferentconcentrationofHClO4solution,extractingtimeandkeepingtimeoftheextractbeforedistillationondeterminationwasstudied1Theresultsshowedthattherecoverywasbetween98142%and101118%,RSDwasbelow1140%,bydeterminatingtheTVBNinaquaticproductsafterthesampleshadbeenhomogenizedfor2minutesat016molL-1HClO4solution1TheresultofthismethodhadnosignificantdifferencecomparedwiththedistillationinGB/T5009144,andtheoperationwaseasyandrapid1Keywords:nitrogenautoanalyzer;aquaticproducts;TVBN;determination(TVBN),,,,,TVBN,[1,2],TVBN[2-4],,,,,,,TVBNTVBN6:51,TVBN111111111,2200()11112(012014016018molL-1),(20gL-1),(30gL-1),,(12gL-11,1gL-1),(0101molL-1),(100mgL-1),,11113(Tilapianiloticus),(Decaplerusmaruadsi),(Litopenae2usvannamei)(Crassostrearivularis),,,11211211,;;20100g,100mL,11212(1L815mL)30mL;50mL;20mL;3min;5s1121320100mL,2,,,,,,,0101molL-120100mL()11213X=(V1-V2)c14m20/100100:XTVBN,mg100g-1;V1,mL;V2,mL;c,molL-1;m,g;141100mL[c(1/2H2SO4=1molL-1)],mg221111211,,2min,,11TVBNTab11InfluenceofHClO4concentrationonTVBNcontentinmusclesoffourspeciesHClO4/molL-1T1niloticusfilterabilityTVBN/mg(100g)-1D1maruadsifilterabilityTVBN/mg(100g)-1L1vannameifilterabilityTVBN/mg(100g)-1C1rivularisfilterabilityTVBN/mg(100g)-1081501918561557148012912021160711081010141011523182719581580161012423185811081620181012523184811281601,,,,TVBN,521,,TVBN016molL-1,,TVBN21211211,016molL-1,2min,2,,2minTVBN,,016molL-12min,213,,,,,,2,016molL-1,,2,32TVBNTab12InfluenceofextractingtimeonTVBNcontentinmusclesoffourspeciesmg(100g)-1/minextractingtimeT1niloticusD1maruadsiL1vannameiC1rivularis010120231958106816515101222410081058168301012524104810781663TVBNTab13InfluenceofkeepingtimeonTVBNcontentinaquaticproductsmg(100g)-1samplemethod/temperature/hkeepingtime012412244896144192T1niloticusHClO45101301013010131101331013610141101501016410183111402010130101321013410137101521017811130121423010130101351014210156101751112512140H2O510122101241012710130101451016611112121782010122101321014610178161633010122101381016211140D1maruadsiHClO452315623157231592316223172231852411024167251252615220231562316023166231772411524173261153023156231642317223191241662518628143H2O523150231542315923168241022415825174281102023150231772411024181271463023150231892415226106:TVBN10%Note:TVBNcontentinextractisnotdeterminatedafterincreasedby10%13,,2TVBN,2TVBN,TVBN56:53144h,2TVBN5114%7117%,48h,2TVBN8181%9153%,2TVBN3,,,TVBN,,210%,56d,203012d214,(NH4)2SO4,112TVBN,(4)98142%101118%,99147%,215112TVBN6(5),6618010-21210410-2,1140%,4Tab14Recoveryofstandardadditionmaterialinsamplesample/mg(100g)-1contentinsample/mg(100g)-1additionofstandardmaterial/mg(100g)-1contentofstandardadditionmaterialinsample/%recoveryT1niloticus10116203010799155405012610012410010817898162D1maruadsi23160204318410111840631539918310012217099110L1vannamei718320271809918740471609914310010616198179C1rivularis8168202816199165404812498190100107110981425Tab15TheprecisionofsampledeterminationsampleTVBN/mg(100g)-1determinatevalueaverageS10-2RSD/%T1niloticus1011610110101281011210116101231011861800167D1maruadsi23174231602315023146231702416823161111360148L1vannamei718071827195717771887196718671971101C1rivularis8165817081528146814281688157121041140541216GB/T5009144TVBN6,,2TVBN6t[5],4tt0195,262TVBNTab16ComparisonofTVBNcontentinsampledeterminedbytwomethodssample3methodTVBN/mg(100g)-1S10-2tt2testsignificanceT1niloticusGB/T5009144101181011361808102t=1116t0195=2123nosignificantD1maruadsiGB/T500914423175231611113614110t=1189t0195=2123nosignificantL1vannameiGB/T50091447186718371978150t=0163t0195=2123nosignificantC1rivularisGB/T5009144815781461210413130t=1150t0195=2123nosignificant:GB/T5009144,,Note:WhentheTVBNwasdeterminatedbythedistillationinGB/T5009144,thecentrifugalseparationwasusedtoseparatetheextractfromthesam2plesolutionbecauseofthedifficultyoffiltration1,,,(1),2minTVBN(2),(3),56d203012d,(4),,5min(5),,,3,,98142%101118%,1140%GB/T5009144,,:[1].[M].:,19891290-2921[2],.[M].:,19871508-5121[3]GB/T5009144220031[S]1[4]CommissionDecisionofEC.Fixingthetotalvolatilebasicnitrogen(TVB2N)limitvaluesforcertaincategoriesoffisheryproductsandspecifyingtheanalysismethodstobeused[J].OfficialJ,1995,L97,84-871[5]1[M].:,19841111