夏天无TLC指纹图谱的研究

[1]1()1:,2000:77927801[2]11:,2001:1791[3],,11:,2003:2381(2005-11-15)TheAnalysisonProcessedChrysanthemumbyFTIRSpectrumsSecond2orderDerivativeSpectrumsandTwo2dimensionalSpectrumsBAIYan1,BAOHong2juan2,WANGDong1,CHENZhi2hong1(11HenanCollegeofTCM,Zhengzhou450008,China;21BeijingUniversityofTCM,Beijing100084,China)AbstractUsingInfraredspectrums,Second2orderderivativespectrumsandTwo2dimensionalspectrumstoanalyzeChrysanthe2mumandprocessedChrysanthemuminhenanprovince,theinfraredcharactersofChrysanthemumandprocessedChrysanthemumcanbefound1KeywordsInfraredspectrum;Sceond2orderderivativespectrum;Two2dimensionalspectrum;Chrysanthemum;ProcessedChrysanthemumTLC1,2,1(11,510515;21,315000):,:TLC,TLC:TLCRr6,,:TLC;TLC;:R28215:A:100124454(2006)0620547204:(19762),,,,:Tel:[phone],E2mail:awag8486@sohu1comCorydalisdecum2bens(Thunb1)Pers1,,12005,:,,,(TLC),,1111METTLERAE240();939();();CS2930();ZF2501();112G();G(013%,20cm10cm013mm),,10530min;G(1%013%,20cm10cm013mm),,10530min,:072629605,;74529620066©1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:11922200101,;,Corydalisdecumbens(Thunb1)Pers12211,(),TLC,22(71541),013%G212,22(71541)G,,,,72%88%213,,=281nm,,,214,,1ml0115mg,;,1ml115mg,215,,015g,,1g,,,1ml,,4h,,2(21),5ml,,,015g,,,2162l,3l,,,,,,2,61616,26,1TLC()A:sample(Xiatianwu)B:protopineC:dl2tetrahydropalmatine21721711:015ml,2ml,,,2l4l6l8l,10l,013%G,,,,30192,0199,21712:2l,013%G,,52RSD9129%,RSD2%2l,013%G,5,5,,RSD(n=5)413%,RSD(n=25)5106%21713:2l,,3h,84529620066©1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.=9102%,5RSD5%,12h21714:015g,,5,,,218A200g/A015g,RSD5%218,2l,3l,,,,,Rr(61),A200g/A015g,1A200g=AS015/W1,As,W1,gA015g=Ar015/W2,Ar,W2,g1TLC16A200g/A015g1(Rr=0)2(Rr=0112)3(Rr=0124)4(Rr=0147)5(Rr=0167)6(Rr=1)1016001510114012301691108011231372015101450113011501550185010721713015501420111012201530184010221694016101520114012401671109011131385014501380110011101490186010521446013301310108012001440181010621227015301480112012101561102010931018014901490111012201541105011031019014401400113012001520199010821781001670153011501250173111101123156:A200g/A015g200g,015g,3311,(s=281nm)(s=281nm,s=370nm),,CS2930,312,;22(71541),,;,,,10212cm313,,,,A200g/A015g,()314A200g/A015g200g,015g,,,,,,,,,,94529620066©1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(),,HPLC,,,,,[1],,11,1994,19(10)61226131(2006-01-15)StudyontheTLC2fingerprintofXiatianwuWEIFeng2huan1,ZHANGJia2jia2,ZHANGLu1(11SchoolofTraditionalChineseMedicine,SouthernMedicalUniversity,Guangzhou510515,China;21ZhejiangPharmaceuticalCol2lege,Ningbo315000,China)AbstractObjective:TodevelopamethodthatcanwellevaluatethequalityofXiatianwu1Methods:MethodsareresearchedanddiscussedtoconstructtheTLC2FP(thin2layerchromatographyfinger2print)ofXiatianwutoevaluateitsquality1Results:Theareasofsixevidentpeaksinthethin2layerchromatographyofXiatianwuarecomparedwiththeareaofdl2tetrahydropalmatinepeakcompaniedwiththeminthesamechromatography1TheRrofeverypeakanditsrelativeareawereacquiredtoevaluatethequalityofXiatianwuandidentifyit1Conclusion:TheTLC2fingerprintofXiatianwucanwellevaluatethequalityofXiatianwu1KeywordsXiatianwu;TLC2fingerprintHPLC1,1,23(11,510282;21,510515):HPLC:MerkLiChrospherRp218e,(pH214),110ml/min,270nm:HPLC:HPLC;HPLC;:R28215:A:100124454(2006)06205502033:ScutellariabarbataD1Don12,34,HPLC,1111(1100series,DAD,Agilent);(T660/H,Elma);(N21000,Eyela);(Simplicity185personal,Millipore);(MerkLiChrospherRp218e:5m,250410mm,NO1447737);:(),()112101,113,,,05529620066©1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.