吸收明胶海绵中明胶交联度的测定

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(,210038):,:2,4,6--,346nm,-,:60%::;;:R917:A:0254-1793(2010)03-0511-02Determinationofcross-linkingdegreeofgelatiningelatinspongeDONGZhi(NanjingJinlingPharmaceuticalFactoryNanjing210038,China)AbstractObjective:Tostudyandimprovethequalityofgelatinsponge,thecross-linkingdegreeofgelatinintheproductisdetermined.Method:Thecross-linkingdegreeofgelatinisdeducedfromthedifferenceofamountof-amino-groupsoflysineinpeptidechainofgelatinbeforeandafterthecross-linkingofgelatinwithformalde2hyde,whichisdeterminedbyusingthereactionbetweenTNBSandtheamino-groupsofaminoacid.Result:Thecross-linkingdegreeofgelatinofdomesticgelatinspongesamplesisabout60%.Conclusion:Themethodappearssuitableforthedeterminationofcross-linkingdegreeofgelatiningelatinsponge.Keywords:cross-linkingdegree;gelatin;gelatinspongeTel:013585176392(025)85801999-8732;E-mail:[e-mail],20[1],,,-[2],[3]1:-(GENERAL,TU-1221,)(,YXQ-LS-75SII,);:2,4,6-(5%,Sig2ma)()();:()()2()11mg,100mL,,50mL,4%1mL015%TNBS1mL,404h,6molL-13mL,1201h,5mL,3,20mL,5mL,15min,15mL,,346nm11mg,50mL115ChinJPharmAnal2010,30(3)©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.=(A01022)/(1146104bX),A,1146104;b,cm;X,g1Tab1Resultofdeterminationofcross-linkingdegreeofgelatiningelatinsponge(samplesnameandbatchesnumber)(samplesweight)/g(absorbance)-(amountofaminogroups)/10-3molg-1-(meanvalueofamountofaminogroups)(cross-linkingdegree)/%(gelatin)08064801011511277013040101151133801319010117112580129501306/(sponge)071211010115014610111001011101380010940101160149401117011076511(sponge)080311010111013990109801011501377010900101140137401090010936917(sponge)09010101011001602011500101060144001114010108015320113501133561644112,4,6-(TNBS)346nm,--,412,,,4136molL-1,,,-TNBS,TNBS6molL-1,-TNBS414TNBS-TNBS4151,-,012018,416,,,,417,,1PENGBi-xian(),CHENGLi-juan().Fromthecollagentothegelatin().TheSciTechnolgelatin(),1994,14(1):12TANGHui-ru().13CNMRStudiesofCross-linkingRe2actionbetweenGelatinandFormaldehyde(-13).ChinaLeather(),1993,22(10):32OfnerIIIClydeM,BubnisWilliamA.Chemicalandswellingevalu2ationsofaminogroupcross-linkingingelatinandmodifiedgelatinmatrices.PharmRes1996,13(12):1821(200946)215ChinJPharmAnal2010,30(3)