LTQ-Orbitrap-Velos_Getting-Started

PartofThermoFisherScientificRevisionA-1250590ThermoFisherScientificLTQOrbitrapVelos™GettingStartedTunePlus2.6©2009ThermoFisherScientificInc.Allrightsreserved.MicrosoftWindowsisaregisteredtrademarkofMicrosoftCorporation.SEQUESTisaregisteredtrademarkofUniversityofWashington.SwagelokisaregisteredtrademarkoftheCrawfordFittingCompany.TeflonisaregisteredtrademarkofE.I.duPontdeNemours&Co.Sigma-AldrichisatrademarkofSigma-AldrichBiotechnology.ProteoMassisatrademarkofSigma-AldrichBiotechnologyLPandSigma-AldrichCo.AllothertrademarksarethepropertyofThermoFisherScientificInc.anditssubsidiaries.ThermoFisherScientificInc.providesthisdocumenttoitscustomerswithaproductpurchasetouseintheproductoperation.Thisdocumentiscopyrightprotectedandanyreproductionofthewholeoranypartofthisdocumentisstrictlyprohibited,exceptwiththewrittenauthorizationofThermoFisherScientificInc.Thecontentsofthisdocumentaresubjecttochangewithoutnotice.Alltechnicalinformationinthisdocumentisforreferencepurposesonly.Systemconfigurationsandspecificationsinthisdocumentsupersedeallpreviousinformationreceivedbythepurchaser.ThermoFisherScientificInc.makesnorepresentationsthatthisdocumentiscomplete,accurateorerror-freeandassumesnoresponsibilityandwillnotbeliableforanyerrors,omissions,damageorlossthatmightresultfromanyuseofthisdocument,eveniftheinformationinthedocumentisfollowedproperly.ThisdocumentisnotpartofanysalescontractbetweenThermoFisherScientificInc.andapurchaser.ThisdocumentshallinnowaygovernormodifyanyTermsandConditionsofSale,whichTermsandConditionsofSaleshallgovernallconflictinginformationbetweenthetwodocuments.ReleaseHistory:RevisionAreleasedinMay2009.SoftwareVersion:Xcalibur2.1orhigher,TunePlus2.6ForResearchUseOnly.Notforuseindiagnosticprocedures.WEEEKonformitätDiesesProduktmussdieEUWasteElectrical&ElectronicEquipment(WEEE)Richtlinie2002/96/ECerfüllen.DasProduktistdurchfolgendesSymbolgekennzeichnet:ThermoFisherScientifichatVereinbarungengetroffenmitVerwertungs-/EntsorgungsanlageninallenEU-MitgliederstaatenunddiesesProduktmussdurchdieseFirmenwiederverwertetoderentsorgtwerden.MehrInformationenüberdieEinhaltungdieserAnweisungendurchThermoFisherScientific,dieVerwerterundHinweisedieIhnennützlichseinkönnen,dieThermoFisherScientificProduktezuidentifizieren,dieunterdieseRoHSAnweisungfallen,findenSieunter’sWasteElectrical&ElectronicEquipment(WEEE)Directive2002/96/EC.Itismarkedwiththefollowingsymbol:ThermoFisherScientifichascontractedwithoneormorerecycling/disposalcompaniesineachEUMemberState,andthisproductshouldbedisposedoforrecycledthroughthem.FurtherinformationonThermoFisherScientific’scompliancewiththeseDirectives,therecyclersinyourcountry,andinformationonThermoFisherScientificproductswhichmayassistthedetectionofsubstancessubjecttotheRoHSDirectiveareavailableatéDEEECeproduitdoitêtreconformeàladirectiveeuropéenne(2002/96/EC)desDéchetsd'EquipementsElectriquesetElectroniques(DEEE).Ilestmarquéparlesymbolesuivant:ThermoFisherScientifics'estassociéavecuneouplusieurscompagniesderecyclagedanschaqueétatmembredel’unioneuropéenneetceproduitdevraitêtrecollectéourecycléparcelles-ci.Davantaged'informationssurlaconformitédeThermoFisherScientificàcesdirectives,lesrecycleursdansvotrepaysetlesinformationssurlesproduitsThermoFisherScientificquipeuventaiderladétectiondessubstancessujettesàladirectiveRoHSsontdisponiblessur™system!TheLTQOrbitrapVelosisamemberofthefamilyofLTQ™massspectrometer(MS)detectors.AboutThisGuideThisLTQOrbitrapVelosGettingStartedmanualprovidesinformationonhowtosetup,calibrate,andtunetheLTQOrbitrapVelos.ProceduresinChapters1–4canbeperformedfromtheXcalibur™TunePluswindow.WhoUsesThisGuideThisLTQOrbitrapVelosGettingStartedmanualisintendedforallpersonnelthatneedtooperatetheLTQOrbitrapVelos,especiallythekeyoperator.Thismanualshouldbekeptneartheinstrumenttobeavailableforquickreference.ScopeofThisGuideLTQOrbitrapVelosGettingStartedincludesthefollowingchapters:•Chapter1:“Introduction”providesgeneralinformationaboutthismanual.•Chapter2:“TunePlusWindow”providesinformationontheTunePluswindow.•Chapter3:“CalibratingtheInstrumentforFTMSMeasurements”providesprocedurestocalibratetheLTQOrbitrapVelosforFTMSmeasurements.•Chapter4:“PerformingDiagnostics/Checks”describesseveraldiagnosticprocedures.•Chapter5:“InstrumentSetup”describestheFTMSrelevanttopicsofthedatadependentsettingsintheInstrumentSetup.•Chapter6:“InstrumentConfiguration”givesinstructionsaboutconfiguringtheinstrument.ReadThisFirstAboutThisGuideiiLTQOrbitrapVelosGettingStartedThermoFisherScientific•Chapter7:“LTQOrbitrapVelosETDInstruments”describestheOrbitraprelevantdifferencesininstrumentsettingsandprocedureswithrespecttousingaLTQOrbitrapVelosETDinstrument.•AppendixA:“MiscellaneousInformation”givesadditionalinformationaboutvarioustopics.ReadThisFirstRelatedDocumenta