COI基因在昆虫中的研究

ChineseJournalofAppliedEntomology20124961687—1695COⅠDNA*＊＊1001932003DNADNAbarcodesDNADNAbarcodingImtDNACOⅠ。DNA、mtDNACOⅠ、NumtsmtDNACOⅠNumtsDNA。DNADNAmtDNACOⅠAdvanceandapplicationofmtDNACOⅠbarcodesoninsectsYANGQian-QianLIZhi-Hong＊＊WUYiLIULi-JunCollegeofAgronomyandBiotechnologyChinaAgriculturalUniversityBeijing100193China．AbstractDNAbarcodinghasbeenwidelyusedintaxonomysincetheconceptappearedin2003．ThemitochondrialgenecytochromecoxidaseICOⅠisregardedasoneofthemostacceptablemolecularmarkersandinsectDNAbarcodingresearchbasedonthisgenehasbeendevelopedinternationally．ThispapergivesageneralintroductiontoDNAbarcodesDNAbarcodingandinsectDNAbarcodeprojects．ItsummarizesresearchintoandtheapplicationofinsectmtDNACOⅠbarcodinginthedescriptionofcrypticspeciesspeciesmolecularidentificationandphylogenetics．InadditionweanalysetheinfluenceofcoamplificationofNumtswithmtDNACOⅠbarcodespresentingseveralsuggestionsondetectingandavoidingNumts．FinallywediscussfutureprospectsforDNAbarcoding．KeywordsDNAbarcodesDNAbarcodingmtDNACOⅠinsect*30971916。＊＊[e-mail]2011-01-252012-02-20DNADNAbarcodesDNADNADNADNAbarcodingHebertetal．2003aHebert2005Miller2007。DNA。、、。DNAcoamplification。DNADNAHebertetal．2004。nuclearmitochondrialpseudogenesNumtsCOⅠ。DNANumtsCOⅠ·1688·ChineseJournalofAppliedEntomology49Numts。1mtDNACOⅠ1.1DNAHebert2003a“”1113320658bpCOⅠDNAStoeckle2003COⅠDNA。2004CBOL170100～20020091iBOL550500DNA。DNADNA2%600bp12Hebertetal．2003a。95%Hebertetal．2003bStoeckle2003。1.2BOLDGuelphbarcodeoflifedatabaseBOLD、、DNA。、、、、6Ratnasingham2007。201012BOLD1074517DNA924413084410。1.3DNADNADNA6、21。DNAINBIPSDNAQBOL3EPPODNA。1.4mtDNACOⅠmtDNACOⅠDNA、、。1.4.1DNADNADNACATB、SDS20092009。Kingetal．2008。DNA2009。1.4.2mtDNACOⅠDNACOⅠ5'14902198Cywinska2006。521.5LepF1/LepR160HebertLCO1490/HCO2198。PCRPCR。、Q-solutionQiagen、DNA2010。COⅠDNACCDBhttp//dnabarcoding．ca/Hajibabaeietal．2005Ivanovaetal．2006。1.4.3mtDNACOⅠ600bp6COⅠDNA·1689·1DNATable1LaunchedinsectDNAbarcodingprogramsBarcodingprogramsLaunchedyearTargetspeciesResearchdevelopmentSpecimensbarcodedSpeciesbarcoded*ReferencewebsiteLepBOL2004165000—48676http//lepbarcoding．org/TrichopteraBOL20071165186452478http//trichopterabol．org/FormicidaeBOL2008122058588796http//．formicidaebol．org/BeeBOL—＞2000066631533BOLDTBI200620002277543BOLDMBI—32007255529BOLD*BOLD201012—。SomeofthedevelopmentdataarefromindependentwebsitesoftheprogramstheotherarefromBOLDhttp//．boldsystems．org/views/login．phpdataendeduptoDec．2010—representslackofrelativeinformation．2mtDNACOⅠTable2CommonprimersinamplifyinginsectmtDNACOⅠbarcodesNameRatioHighMediumLowSequence5'－3'TaxonomicgroupsReferenceLCO1490HCO219866.08%17.48%16.44%GGTCAACAAATCATAAAGATATTGGTAAACTTCAGGGTGACCAAAAAATCAVariousphylaFolmeretal．1994LCO1490-t1HCO2198-t166.05%12.23%21.71%TGTAAAACGACGGCCAGTGGTCAACAAATCATAAAGATATTGGCAGGAAACAGCTATGACTAAACTTCAGGGTGACCAAAAAATCALepF1LepR173.72%13.21%13.07%ATTCAACCAATCATAAAGATATGGTAAACTTCTGGATGTCCAAAAAATCAInsectsHebertetal．2004MLepF1LepR1*63.78%14.63%21.59%GCTTTCCCACGAATAAATAATAAmphibiansHajibabaeietal．2006MlepR1LepF1*62.91%15.63%21.45%CCTGTTCCAGCTCCATTTTC*BOLD。InbracketsisanotherprimerusedratiowascalculatedaccordingtodatafromBOLDhttp//．boldsystems．org/views/primerlist．php．·1690·ChineseJournalofAppliedEntomology49PCRDNA。distancebasedmethodscharacterbasedmethods、BLASTDNA、。DeSalleetal．2005Kohler2007Rachetal．2008。2mtDNACOⅠDNAFosteretal．2004Monaghanetal．2005Rachetal．20082006HoggandHebert2004Balletal．2005。mtDNACOⅠ、。2.1crypticspeciesDNAKohler2007Waugh2007。Hebert20042500DNA10。。DeWaard2009DNA42VershininaLukhtanov2010COⅠ2。Smith20086259717114236May-Itzá20102DNA22。Foottit2009DNA658bpCOⅠ17、。Smith200620TachinidaeDNA173152007DNA16Smithetal．2007。2.2DNADNA、siblingspecies。2011DNA2vanVelzen2007DNA2007DNA。mtDNACOⅠ、。2.2.1、、2010。DNA6COⅠDNA·1691·。Nelson2007COⅠ9Chrysoma56K2PCOⅠDawney2007COⅠGenBankCOⅠBoehme2010DNA658bpCOⅠ6Phoridae。2.2.2。。DNA、。Foottit200813300DNA96%0.2%2009DNAPCRPCR-RFLP、COⅠ。27ISPM27Floyd2010DNAUSDADNAEpiphyaspostvittana。2.3208090。DNACOⅠ2002。DNAHebert2005Hajibabaeietal．2007DNACOⅠ、Luntetal．1996ZhangandHewltt1996。DNAAcsetal．2009Wangetal．2010Fengetal．2011。Smith2005DNA2010DNACOⅠ11mtDNACOⅠ0～0.0460.012。3NumtsCOⅠSongetal．2008。DNADNA。mtDNANumtsBensassonetal．2001RichlyandLeister2004。NumtsDNA。3.1NumtsCOⅠ、、、Numts。Pamilo2007、、4NumtsNumts20004Numts·1692·ChineseJournalofAppliedEntomology491kb1.0bpPamiloetal．2007Song2008NumtsNumtsNumtsNumts。NumtsNumtsmtDNACai2010DNAmtDNADNA3COⅠCOⅠNumtsCOⅠ。3.2NumtsNumtsmtDNA、、PCRNumts。Numts、COⅠSongetal．2008。DNACOⅠNumtsYuanetal．1999DNANumtsBensassonetal．2001Numts750bpPCRRT-PCRHebertetal．2004PereiraandBaker2004Moultonetal．2010。44.1DNA、、DNA3。DNA。DNADNAKohler2007。DNA。DNAWillandRubinoff2004。COⅠ。DNA。201016SrRNACOⅠDNA。16SrRNACOⅠDNACytb12SrRNAND618SrRNAITS。。mtDNACOⅠNumts。NumtsDNANumts。NumtsNumtsDNAMagnaccaandBrown2010。4.2DNAmini-barcodesDNA100bpHajibabaeietal．2006。Meusnieretal．20082009Leesetal．2010DNA6COⅠDNA·1693·。DNADNA。ReferencesAcsZChallisRBihariPBlaxterMHaywardA2009．PhylogenyandDNAbarcodingofinquilineoakgallwaspsHymenopteraCynipidaeoftheWesternPalaearctic．Mol．