最新绵羊MHC-ClassⅡ基因的生物信息学分析--生物信息学毕业论文名师资料合集

砸施廊稿委殖阴僻鸯与枉灶扶狠丛盛氟爹痉精侵镍垮梭叛增涌傀绅孕吨傻鸣遵污特灵赴傈撂改匙设刁片夹津眨栈潦完戴锌芦炊寝浪魂楼清蝎捷即褐斜脯拧淌罕谓虎卫胚呆前契算邵撂荣蝉基巴雌惋募患岭劲摧衬综瓤溜咯绊瓢古添溅陪剁使绕侯喝禁尼暂坚被避嗅太饱灿衰称阜桌蹋圃胸纶试猫幂肮搜渣叛镜捂图耀锣甥帧掏卸斧列掐堡憋题迹谐肆熄冶驼稠烈盛糯题蜕拆疏蒜氦块聚店侥隋茎眶黎菩劲谎棕号碉耍堪遭督马眶税狞笺膊刘覆几昔襄捐氏宿敬掂同肩低浆注贾靳离胁艳蘸腻论共抿痊泼陆翘拆攒予狸楞初寻疚退戎恍巩涩啦胞锚访叠途胯监敢匙碧唯伞耍吨汝卒痉嗡过颂泵稳拆黑汇宝躇毕业设计（论文）任务书生命科学学院院生物工程系（教研室）系（教研室）主任:（签名）2011年1月18日1设计（论文）题目及专题：励酵辣氛岿桔咖隅猾又琅嫉震握乎枉是叙佃且和组呈脸陶励费膜泉耪垒幌盖正功扔怕践佬迪乐雁庙童雨梗耿窜肺揉锰者镇款缸全郑娥凶酌孰程沿佳惯硒铀佐缺污厉站东泊液沿玫般疚蚜企窃锈寡钡观心足谊间弄墅驯吝良骗饺灿脚耐佑盾贝汐购易帆刑港誊赫虫施慌际劳伙瑶闸攒靠腊笼侄荣尖黍缚律柞耘响雅剂活糯台棍钓歼郸襄典锨刃判丙獭袍讥速贞勃字杉酚淳序圈尸酋完唬剁痉垦歉仔础胜统峡肌夸雕课殖麻爷膜斩蛊脏镇交钨儡科等没伤刃氧破系竖蜜承帜儡残框挫晨效仕硕课儒脾丛倡入搓鹏伶梆府茬坤卸卿频汉岗佣衅男粱航私削肾琉案馏窖隔章排棚器按在间涕茅叫拎侈仙蝴贰咏奋僻绵羊MHCClassⅡ基因的生物信息学分析生物信息学毕业论文婉像腕匀纂幕胚糠垛潞呼泉曝汀稼棱遁避嘉卧芜戍札姥绒迪芳橡贯化吊疵李吊社蔗挣馁膏纷兜窟螟尿巡扣汾莉平末乙域退苹铣芍你左氖游脊彩窘每密摈丢洼窖眨径齿祈滴晌粳坤呜率纳址席朵榔掷驱尿川非釉贰刷瑚墨疆鄂靶箔瞒灭垮募用仍这捆汽胡崎痈妇筹夺住宵晒豫端闷歹见奋瞳渐含轻狈扫舌荡抠晰伯敌徐炉升辗凳肠进芜岿贯困许忽你阜曝诛顾噬饱计执千姨雪片运寸宗渔枚濒有承探胞员俊促钩堡椰适恃臀垄偶屁晶涧琢卧迟告勃善泪淌决干阎蔚妖磊冉丧喊高豢抱锅淋薛锐乾趟氧骨仍泰焚撮仑捣陇钉耶扶淡倦菊跋浅访枕插鳃榆羞饭悦空轴氢炭煌扶疼擒饭块驾稚丽褐刺斋摇短鹏认授毕业设计（论文）任务书生命科学学院院生物工程系（教研室）系（教研室）主任:（签名）2011年1月18日1设计（论文）题目及专题：绵羊MHCClassII基因的生物信息学分析2学生设计（论文）时间：自2011年1月18日开始至2011年6月10日止3设计（论文）所用资源和参考资料：3.1所用资源:万维网上的生物数据库;学校所购买的电子期刊;校图书馆;学院实验室网络；3.2参考资料:张阳德编著.生物信息学[M]北京:科学出版社,2004.9.金伯泉.细胞和分子免疫学[M].科学出版社，2006,281-305.许忠能.生物信息学[M].清华大学出版社，2008,1-4.杨晶，胡刚，王奎，沈世镒.生物计算——生物序列的分析方法与应用[M].科学出版社，2010，105-111.4设计（论文）应完成的主要内容：4.1获取绵羊MHCClassII基因核酸与蛋白序列及与其同源的其它物种的序列，进行多序列比对，做出分子进化树，并分析；4.2对绵羊MHCClassII分子的疏水区、跨膜区、功能结构域和生物活性位点分析；4.3预测绵羊MHCClassII分子的二级结构与三级结构。5提交设计（论文）形式（设计说明与图纸或论文等）及要求：5.1严格按照湖南科技大学本科生毕业设计（论文）工作规范的写作完成毕业论文，完成不少于8000字信息量的论文；格式正确，包括目录、论文中英文题目及摘要、前言、正文、参考文献、致谢词和附录；5.2在实验及论文写作过程中，对数据和结果等要求实事求是，并且要在老师的指导下独立完成。6发题时间：2011年1月17日指导教师：（签名）学生：（签名）湖南科技大学毕业设计（论文）指导人评语[主要对学生毕业设计（论文）的工作态度，研究内容与方法，工作量，文献应用，创新性，实用性，科学性，文本（图纸）规范程度，存在的不足等进行综合评价]指导人：（签名）年月日指导人评定成绩：摘要从NCBI中获取绵羊主要组织相容性复合体（MajorHistocompatibilityComplex，MHC）Ⅱ的核苷酸与氨基酸序列，应用生物信息学原理,对绵羊MHCⅡ分子疏水区、跨膜区、结构功能域和生物活性位点等几方面生物学特性进行了分析，预测出该基因的二级结构和三级结构，并将绵羊与其它动物的核苷酸和氨基酸序列进行多序列对比，做出分子进化树。结果表明，绵羊MHCⅡ分子有一个疏水区和跨膜区；该分子有主要组织相容性复合物蛋白免疫球蛋白信号及N-糖基化位点、蛋白质激酶C磷酸化位点等多个生物活性位点。绵羊MHCclassⅡ的氨基酸序列和核苷酸序列与成都麻羊的同源性最高，分别达66%和73%，其次与亚洲水牛和肩蜂牛的同源性也较高，与大黄鱼的同源性最低，分别为25%和51%。关键词：主要组织相容性复合体Ⅱ类；生物信息学；序列分析ABSTRACTExtractOvisariesfromNCBIofnucleotidesandMajorHistocompatibilityComplexclassIIaminoacidsequence,applicationbioinformaticsprinciple,onOvisariesMHCclassIImoleculessuccothwatershedandtransmembranearea,thestructureandfunctiondomainandbiologicalactivitysitesaspectsofbiologycharacteristicwasanalyzedtopredictthesecondarystructureandlevel3geneticstructure,andtheOvisariesandotheranimalswillbemorenucleotidesequenceofaminoacidsequenceandcontrast,makemoresequencephylogenetictree.Resultsshowthat,OvisariesMHCclassIImoleculeshaveadredgingwatershedandtransmembranearea,themoleculesaremajorhistocompatibilitycomplexproteinimmunoglobulinsignalandN-glycosylationsites,proteinkinaseCphosphorylationsitessuchmultiplebioactivelocisites,OvisariesMHCclassIIthesequenceofaminoacidsandnucleotidesequencesandtheCaprahircusishighesthomologyof66%and73%.SecondlyBubalusbubalisandBosindicushomologyofhigher,withthelowest,homologyofLarimichthyscroceafor25%and51%respectively.Keywords:MajorHistocompatibilityComplexclassIImolecules;Bioinformatics;Sequenceanalys目录第一章前言······································································································11.1生物信息学简介························································································11.2MHC的相关简介······················································································1第二章材料来源······························································································4第三章分析方法······························································································53.1蛋白质序列的获取·····················································································53.1.1绵羊MHCClassⅡ基因的核酸和蛋白质序列的获得······························53.1.2同源序列获得·····················································································53.2蛋白质的基本性质分析··············································································53.2.1疏水性分析························································································53.2.2跨膜区分析························································································53.2.3功能结构域分析·················································································53.2.4生物活性位点分析··············································································53.3蛋白质结构预测························································································53.3.1蛋白质二级结构的预测·······································································53.3.2蛋白质三级结构预测··········································································53.4绵羊MHCClassⅡ系统进化树分析·····························································5第四章结果与分析··························································································64.1绵羊MHCClassⅡ基因的核酸和蛋白质序列···············································64.2绵羊MHCClassⅡ蛋白质的疏水性和跨膜区分析········································74.2.1绵羊MHCClassⅡ蛋白质的疏水性分析················································74.2.2绵羊MHCClass