急性大鼠脊髓损伤Allen-s法模型的改良及电生理评价-李一帆

ducedchangesinstructureanddynamicsofhumanphosphomevalonateki-nase[J].TheFASEBJournal,2008,22(14):1012.[8]SabrinaP,MartaLetiziaH,ElisabettaF,etal.TheFunctionalQ84RPoly-morphismofMammalianTribblesHomologTRB3IsAssociatedWithInsulinResistanceandRelatedCardiovascularRiskinCaucasiansFromItaly[J].Diabetes,2005,54(9):2807.[9]CraveroJD,etal.IncreasedexpressionoftheAkt/PKBinhibitorTRB3inosteoarthriticchondrocytesinhibitsinsulin-likegrowthfactor1-mediatedcellsurvivalandproteoglycansynthesis[J].ArthritisRheum,2009,60(2):492.(:2009-08-19):(30970739)(20090726)＊:1007-4287(2010)08-1169-04Allen's李一帆1,2,陈　东1,3＊,张大威4,薛　辉1,刘佳梅1(1.,130021;2.;3.;4.):　、、,(spinalcordinjury,SCI)。　36Wistar,12。(SCI):Allen's,60gcmT10。:,,SCI。:,。(BBB),30。　HE:。SCI、,、。BBB:1。SCI2,3,BBB6,。(SEP,MEP):SCISEPMEP-,,(P0.01)。　、,。:;;BBB;;:R683.1;R687.32:AImprovementandeletrophysiologyassessmentoftheratsallen'smodelforacutespinalcordinjury　LIYi-fan,CHENDon,ZHANGDa-wei,etal.(NormanBethuneSchoolofBasicMedicalScience,JilinUniversity,Changchun130021,China)Abstract:Objective　Toestablishamorepractical,standardanddependablemodelforratsacutespinalcordinjuryandtolaythefoundationoffurtherresearchonspinalcordinjury(SCI).Methods　Thirty-sixfemaleratswererandomlyassignedto3groups(n=12pergroup).SCIgroup:SCIwascreatedwithamodifiedimpingerofAllen'sbya60gcmimpactingontheT10spinalcord.Shamgroup:neuralscutewasopenedonlyandspinalcordwasexposedwithoutSCI.Normalcontrolgroup:normalrats.Ethologicalobservation(BBBscores)wasdoneregularly.Histologicalandelectrophysiologicaltestsweredoneonthe30thdayafteroperation.Results　HEstainingresultsinShamgroupandNormalcontrolgroupwereidentical,butSCIgroupshowedfragmentedconstructioningreymatterandnecrosis,andcellularswellingwasdetectedininjuredregion.BBBscoresrevealedthatnearlyfullfunctionalrecoveryafteroperationinShamgrouprequired1Week.InSCIgroupfunctionalrecoverybeganin2ndweekafteroperationandstopin3rdweek,andthescoresfailedtoexceed6inthefinal.SignificantdifferencelaybetweenShamgroupandSCIgroup(P0.01).Electro-physiological(SEP,MEP)demonstratedthatpeak-to-peakvaluedroppedsharplyandlatencywasextendedobviouslyinSCIgroup.Conclusion　ThismethodcanberegardedasasimplerandidealwaywhichwasingoodreproducibilitytoestablishtheratsmodelforacuteSCI.Keywords:Ratsmodel;Spinalcordinjury;BBBscore;Electrophysiology;Modification(ChinJLabDiagn,2010,14:1169)　　(spinalcordinjury,SCI)、,,,—1169—　20108　14　8,[1,2]。、SCI,SCI。Allen's,、。1　1.1　:Allen's,,4.0mm,,20g,4.1mm,,,3-10cm。1.2　36200～250gWistar,。,20～23℃,50%～65%,3d。,12。,;,,,SCI;(SCI),。1.3　。Wistar,200～250g,10%(0.35ml/100g),,,(13),、5cm、、,,,3cm,、,,,,,10,、。:T9,T10,T11[5]。T10,T9T10、T10T11,T10,,,T10“”,T10,,,T10。Allen's,,,,,,20g3cm,60g·cm,T10,,,,3min。:、,,,,,,。。7d,7d5。,,,20～23℃,50%～60%。8U/,0.2U/,,7d。,,,,,。,,,。2,。2～3d。1,SCI20d,,SCI1。1.4　1.4.1　　30d,6,10%,,、4%,T8～T12,,4%4h,,OCT10min,,,-70℃。,16μm,HE。1.4.2　　BassoBBB[6,7](21),,3。1.4.3　　30d,9,(SEP)(MEP)。10%,,,,,3mm,1mm;,。Powerlab,BL-410,:,3V,1ms,10Hz,20,300Hz,0.01s,6.25ms/div。(SEP),,,,100,;(MEP),—1170—ChinJLabDiagn,August,2010,Vol14,No.8,。-,。1.5　SPSS10.0。±,,P0.01。2　2.1　HE、,、、、,,。SCI、,、、、,(1、2)。2.2　BBB21。1,,2,,1,BBB20,2BBB21。,,,。SCI,,。1,2,,3,3,SCI(P0.01),3d(1)。1　,　2　SCIHE×100HE×1001　BBB(n=12,x—±s)3691215182124273021＊#21＊21＊21＊21＊21＊21＊21＊21＊21＊9.42±0.37＊19.56±0.76＊20.13±0.82＊20.88±0.11＊21＊21＊21＊21＊21＊21＊SCI00.68±0.591.85±0.682.42±1.033.71±1.154.69±1.465.14±1.275.56±1.035.56±1.035.56±1.03　　＊P0.01vs.SCIgroup;#P0.01vs.Shamgroup2.3　2、33、4SEPMEP-,、,SCISEPMEP-,,,(P0.01)。2　SEP--(n=9,x—±s)(mS)-(mV)2.06±0.02＊0.16±0.01＊2.05±0.02＊0.15±0.01＊SCI5.23±0.260.03±0.01　　＊P0.01vs.SCIgroup3　MEP--(n=9,x—±s)(mS)-(mV)2.02±0.02＊80.79±0.55＊2.01±0.02＊80.76±0.53＊SCI4.73±0.355.95±1.03　　＊P0.01vs.SCIgroup　　3　SEP　　4　MEP3　Allen's、,,SCI,,Allen's,,,SCI[8]。。—1171—　20108　14　8Allen's,4.0mm,4.1mm,,,,3min,SCI,;,,3-10cm,,,SCI。,,,T6-T12。T10,;T10,。“”,,,,,,,,、、。(SEP)(MEP),SCI。Singer(1970),SCI[9],SCI[10]。MEP,,[11];SEP,。50%,10%[12]。,,,-。,,,。SEPMEP,SCI,,MEPBBB,SCIBBB,,。,。,Allen's、、、。、、、,,,。,100%。,、、,、,,。:[1]BaydinA,CoklukC,AydinK.Anewminimallyinvasiveexperimentalspinalcordinjurymodelinrabbits[J].MinimInvasiveNeurosurg,2007,50(3):170.[2]FukudaS,NakamuraT,KishigamiY,etal.Newcaninespinalcordinjurymodelfreefromlaminectomy[J].BrainResBrainResProtoc,2005,14(3):171.[3]AllenAR.Surgeryofexperimentallesionofspinalcordequivalenttocrushinjuryoffracturedislocationofspinalcolumn:Apreliminaryreport[J].JAMMedAssoc,1911,57:878.[4]AllenAR.Remarksonthehistopathologicalchangesinthespinalcordduetoimpacts:Anexperimentalstudy[J].JNeuNintDis,1914,41:141.[5],,　,.[J].,2008,22(8):933.[6]BassoDM,BeattieMS,BresnahanJC.Asensitiveandreliablelocomotorratingscaleforopenfieldtestinginrats[J].JNeurotrauma,1995,12(1):1.[7]Engesser-CesarC,AndersonAJ,BassoDM,etal.Voluntarywheelrunningimprovesrecoveryfromamoderatespinalcordinjury[J].JNeurotrauma,2005,22(1):157.[8]DelamarteRB,ShermanJ,GrrrJB.Pathophysiologyofspinalcordinjury.Recoveryafterimmediateanddelayeddecompression[J].JBoneJointSurg,1998,77-A:1042.[9]CaoQ,ZhangYP,IannottiC,etal.Functionalandelectrophysiologicalchangesaf