牙周组织再生技术的研究进展

··2012－01－181985－。[e-mail]710032。、。。R780．2A1005－2593201209－0537－062012229537ProgressinthestudyofperiodontaltissueregenerationGAOLi－naCHENFa－mingJINYanSchoolofStomatologyTheFourthMilitaryMedicalUniversityXi'an710032ChinaAbstractPeriodontitisisaperiodontaltissueinfectiousdiseaseandthemostcommoncauseoftoothlossinadults．Italsohasbeenlinkedtomanysystemicdisorderssuchascoronaryarterydiseasestrokeanddiabetes．Withtheresearchprogressofperiodontaldiseasepathogenesisperiodontaltreatmenthasbeenshiftedfromstoppingdiseaseprogressiontopromotingperiodontaltissuereconstructionandregeneration．Inthispaperthedevelopmentofperiodon-taltissueregenerationtechnologyanditsprospectarereviewed．KeywordsperiodontiumregenerationtissueengineeringcellsheetcelltransplantationChineseJournalofConservativeDentistry2012229537。、、、、。、。。1、。。。、、1－2。、·735·ChinJConservDent20122293－4。5－6。2、、。、、。。。。2．17－1011、12、。13－14。、、。、、、、。。Mcgire152。2．2、。、16－17。18PDLCsnHACPDLCs－nHAC、。①②、③。、。。。2．3cellsheetengineering、。。。、、·835·ChinJConservDent201222919。2．3．1、。NagaiN202004。。。C21C。ECM。22PNIPAAm32℃－PNIPAAmTCPs。－EDTA。－。2．3．2、、、、、。Hasegawa23CPDLCs3－。。、。PDLCsFlores242008PDLSCsPDLSCs。Flores25。。Akizuki26BeagleC2Beagle83、。Iwata272009PGAβ－、。、。282009－APTG－CMPDLSCs、CBB6。·935·ChinJConservDent2012229。、、29。Handa304。BaiYudi31HERSDFC1Icol－1、ALP、runt2Runx2、BSPmRNA3ALP、OCN、BSP、OPGDFCsDFCs。HERS3DFC5HERSDFC。HERSDFC－。32、33、34、3536、37－3816、。。39TranswellBMMSCsPDLSCs－。－。33．1。。4012。。3．2。、。。、、。3．3“”、extracellularmatrixECM、。、、、。·045·ChinJConservDent2012229、、、。1AlghamdiASShiblyOCiancioSG．OsseousgraftingpartIautograftsandallograftsforperiodontalregeneration－aliteraturereviewJ．JIntAcadPeriodontol201012234－38．2AlghamdiASShiblyOCiancioSG．OsseousgraftingpartIIxenograftsandalloplastsforperiodontalregeneration－aliteraturereviewJ．JIntAcadPeriodontol201012239－44．3MurphyKGGunsolleyJC．Guidedtissueregenerationforthetreatmentofperiodontalintrabonyandfurcationdefects．Asys-tematicreviewJ．AnnPeriodontol200381266－302．4VillarCCCochranDL．RegenerationofperiodontaltissuesguidedtissueregenerationJ．DentClinNorthAm201054173－92．5HollyDMracnaJ．TheuseofplateletrichplasmawithguidedtissueregenerationindefectscausedbyperiodontaldiseasesJ．BratislLekListy200911010669－671．6SugaiKSatoSSuzukiKetal．IntentionalreimplantationofatoothwithsevereperiodontalinvolvementusingenamelmatrixderivativeincombinationwithguidedtissueregenerationandbonegraftingacasereportJ．IntJPeriodontRestoratDent200828189－94．7BortinMMGaleRPKayHEetal．Bonemarrowtransplanta-tionforacutemyelogenousleukemia．FactorsassociatedwithearlymortalityJ．JAMA198324991166－1175．8SpeckBBortinMMChamplinRetal．Allogeneicbone－mar-rowtransplantationforchronicmyelogenousleukaemiaJ．Lan-cet198418378665－668．9ThomasEDBucknerCDBanajiMetal．Onehundredpa-tientswithacuteleukemiatreatedbychemotherapytotalbodyirradiationandallogeneicmarrowtransplantationJ．Blood1977494511－533．10WaltersMCPatienceMLeisenringWetal．BonemarrowtransplantationforsicklecelldiseaseJ．NEnglJMed19963356369－376．11FoxIJChowdhuryJRKaufmanSSetal．TreatmentoftheCrigler－NajjarsyndrometypeIwithhepatocytetransplantationJ．NEnglJMed1998338201422－1426．12LindvallO．Parkinsondisease．StemcelltransplantationJ．Lancet2001358SupplS48．13WollertKCMeyerGPLotzJetal．Intracoronaryautologousbone－marrowcelltransferaftermyocardialinfarctiontheBOOSTrandomisedcontrolledclinicaltrialJ．Lancet20043649429141－148．14MenaschePHagegeAAScorsinMetal．Myoblasttransplan-tationforheartfailureJ．Lancet20013579252279－280．15McguireMKScheyerET．Arandomizeddouble－blindplace-bo－controlledstudytodeterminethesafetyandefficacyofcul-turedandexpandedautologousfibroblastinjectionsforthetreat-mentofinterdentalpapillaryinsufficiencyassociatedwiththepa-pillaprimingprocedureJ．JPeriodontol20077814－17．16PalmerRMCortelliniP．Periodontaltissueengineeringandre-generationconsensusreportoftheSixthEuropeanWorkshoponPeriodontologyJ．JClinPeriodontol2008358Suppl83－86．17ChenFMJinY．Periodontaltissueengineeringandregenera-tioncurrentapproachesandexpandingopportunitiesJ．TissueEngPartBRev2010162219－255．18．－J．2004293189－192．19Elloumi－HannachiIYamatoMOkanoT．Cellsheetengineer-ingauniquenanotechnologyforscaffold－freetissuereconstruc-tionwithclinicalapplicationsinregenerativemedicineJ．JIn-ternMed2010267154－70．20NagaiNYunokiSSatohYetal．Amethodofcell－sheetpreparationusingcollagenasedigestionofsalmonatelocollagenfibrillargelJ．JBiosciBioeng2004986493－496．21ShigaMKapilaYLZhangQetal．Ascorbicacidinducescollagenase－1inhumanperiodontalligamentcellsbutnotinMC3T3－E1osteoblast－likecellspotentialassociationbetweencollagenaseexpressionandchangesinalkalinephosphatasephe-notypeJ．JBoneMinerRes200318167－77．22OkanoTYamadaNSakaiHetal．Anovelrecoverysystemforculturedcellsusingplasma－treatedpolystyrenedishesgraftedwithpolyN－isopropylacrylamideJ．JBiomedMaterRes199327101243－1251．23HasegawaMYamatoMKikuchiAetal．HumanperiodontalligamentcellsheetscanregenerateperiodontalligamenttissueinanathymicratmodelJ．TissueEng2005113－4469－478．24FloresMGHasegawaMYamatoMetal．Cementum－perio-dontalligamentcomplexregenerationusingthecell