化脓隐秘杆菌毒力因子的研究进展

,,,,(,110161):S852.61:A:052926005(2010)0120052202(Arcanobacteriumpyogenes),,[1],,,,4,(PLO)(CbpA)(Nan)(Fim),11.1(Pyolysin)PLO,57.9kDa,ploplo1605plo,PLO,,PLOPLO,,BillingtonSJ[2]1997,,PLO;,JostBH[3]1999PLO,;20033HIS2PLO.F(497):2009201212:(30972214);(20082126);(2008641):(19832),,,,E2mail:[e-mail]:,E2mail:[e-mail](499)HIS2PLO.A(522),,3,,[4]PLO,30%40%PLO,,PLO,1.2(collagen2bindingprotein,CbpA),,PaulaAE[5],,FarWesternblottingCbpACbpAcbpA,3500,124.7kDaCbpACbpAN2(A)C2(B)CbpA,,,6CbpA(HIS2CbpA)IIIIV,,CbpAHeLa3T6,cbpA38.2%57.0%HIS2CbpA,cbpA48%,CbpA,[5],CbpACbpA[6]1.3,(neuraminidase,Nan),NanHNanP,nanHnanPNan,,;252010(46)1ChineseJournalofVeterinaryMedicine,;IgA,;nanH3009,107kDaNanHNanHNanNanHnanHNan,NannanPnanH,5112,NanP186.8kDaBBR1nanPNan,;nanHnanP,NanNanH,NanP;NanH,NanP64.2%[7]1.4,,4,FimAFimCFimEFimG4fimAfimCfimEfimG,fimA,fimE98%,fimCfimG67%[8],1.5,4,,[9](fibrinogen2bindingprotein),(PMNs);(Fibronectin2bind2ingprotein),,,:(1)DNA,,(2)(Proteases),,;IgA,,2SilvaE[8]BOX2PCR,,,8(PLOCbpANanHNanPFimAFimCFimEFimG),,8,,,,3,;CbpA,,,[9],,:[1]QueenC,WardACS,HunterDL.BacteriaisolatedfromnasalandtonsillarsamplesofclinicallyhealthyRockyMountainbighornanddomesticsheep[J].WildlDis,1994,30(1):127.[2]BillingtonSJ,JostBH,CuevasWA,etal.TheArcanobac2terium(Actinomyces)pyogeneshemolysin,pyolysin,isano2velmemberofthethiolactivatedcytolysinfamily[J].Bacteri2ol,1997,179(19):610026106.[3]JostBH,SongerJG,BillingtonSJ.AnArcanobacterium(Actinomyces)pyogenesmutantdeficientinproductionofthepore2formingcytolysinpyolysinhasreducedvirulence[J].In2fectImmun,1999,67(4):172321728.[4]JostBH,TrinhHT,SongerJG,etal.ImmunizationwithgenetictoxoidsoftheArcanobacteriumpyogenescholesterol2dependentcytolysin,pyolysin,protectsmiceagainstinfection[J].InfectImmun,2003,71(5):296622969.[5]PaulaAE,BillingtonSJ,MalenAL,etal.TheArcanobac2teriumpyogenesCollagen2BindingProtein,CbpA,PromotesAdhesiontoHostCells[J].InfectImmun,2003,71(8):436824374.[6]PietrocolaG,ValtulinaV,RindiS,etal.FunctionalandstructuralpropertiesofCbpA,acollagen2bindingproteinfromArcanobacteriumpyogenes[J].Microbiology,2007,153(10):338023389.[7]JostBH,SongerJG,BillingtonSJ.IdentificationofasecondArcanobacteriumpyogenesneuraminidaseandinvolvementofneuraminidaseactivityinhostcelladhesion[J].InfectImmun,2002,70(3):110621112.[8]SilvaE,GaivÃoM,LeitÃoS,etal.GenomiccharacterizationofArcanobacteriumpyogenesisolatesrecoveredfromtheute2rusofdairycowswithnormalpuerperiumorclinicalmetritis[J].VetMicrobiol,2008,132(122):1112118.[9]JostBH,BillingtonSJ.Arcanobacteriumpyogenes:molecu2larpathogenesisofananimalopportunist[J].AntonievanLeeuwenhoek,2005,88(2):872102.35ChineseJournalofVeterinaryMedicine2010(46)1