高效价人绒毛膜促性腺激素的制备新方法

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(6):580-5811,2,1,1,13(1,210029;2,210029):(HCG):BaCO3HCG:3000IU/mgHCG8000IU/mgHCG,63181%:,,;;;BaCO3TQ467.94A1000-5048(2008)06-0580-02AnewpreparativemethodofhumanchorionicgonadotropinwithhightiterZHANGRi2hua1,2,LINGJia2jian1,WANGNing1,WANGLin2tao131DepartmentofBiochemistryandMolecularBiology,NanjingMedicalUniversity,Nanjing210029;2DepartmentofGeratology,theFirstAffiliatedHospitalofNanjingMedicalUniversity,Nanjing210029,ChinaAbstractAim:Toimprovethepurificationmethodofhumanchorionicgonadotropin(HCG)withhightiter.Methods:CombinativeapplicationofrivanolprecipitationmethodandBaCO3adsorptiveprocesswasusedtopurifyHCGoflowtiter.Results:TheproposedmethodallowedthepurificationofHCGof3000IU/mgto8000IU/mg.Theaveragerecoveryofbiologicalactivitywasfoundtobe63.81%.Conclusion:Thismethodissim2ple,low2costandpractical.Keywordshumanchorionicgonadotropin(HCG);preparation;rivanol;BaCO3(humanchorionicgonado2tropin,HCG),HCGTSH()FSH()LH()[1],HCGFSHLHHCGHCG(10IU/mg)3000IU/mg[2],HCG(8000IU/mg)1(HCG)3000IU/mg,[2];(rivanol,);HCG();HCG();RC5C(Dupot)2211HCG100mg50mL,,1%,3000r/min15min,595%,,3000r/min10min3,,212BaCO3HCG,012mol/L085320082012303Tel:[phone]E2mail:wang_lintao@hotmail1com©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(Ac)220mL,,3LHCG50mL,2mol/LNa2SO4,BaSO43000r/min10minBaSO4,115L213[3]3311BaCO3HCG(2846IU/mg)20mg3,1Table1PurificationofHCGBatchNo.RoughHCGWeight/mgBA/(IU/mg)PurifiedwithrivanolWeight/mgBA/(IU/mg)Activityrecovery/%PurifiedwithBaCO3Weight/mgBA/(IU/mg)Activityrecovery/%1202846111434154831427198503570159220284611154396380135815748927316632028461215538658512271515263691441,,,,,HCG,,HCG,BaCO3,HCG,,HCG,,,,HCG312BaCO3HCG(3253IU/mg)100mg3,2Table2PurificationofHCG(purifiedwithrivanolandBaCO3)BatchNo.PurifiedHCGWeight/mgBA/(IU/mg)Activityrecovery/%125119823463176225103785360142326179816567124HCG2500IU/mg,,3000IU/mgHCG4000IU/mg1,BaCO32500IU/mgHCG,4000IU/mgHCG,HCG(8000IU/mg),,[4-5],,HCG4BaCO3HCG,,,HCG,[1]TalwarGP,SinghO,PalR,etal1Avaccinethatpreventspreg2nancyinwomen[J]1ProcNatlAcadSciUSA,1994,91(18):8532-85361[2](WangLT),(DingY),(XuXL)1[J]1(JChinaPharmUniv),2001,32(5):392-3951[3]1()[M].:,2000:5421[4]CanfeldRE,MorganFJ,KammermanS1Studiesofhumanchori2onicgonadotropin[M]1AstwoodEB.RecentProgressinHor2maneResearch.AcademicPress,NewYorkandLondon,1971,27:121-1541[5]BahlOP.HumanChorionicGonadotropin[J].BiolChem,1969,244(3):567-5641185