CFX96_CFX384_荧光定量PCR说明书

CFX96™CFX384™PCR#184-5384#185-5384#184-5096#185-5096©2008Bio-RadLaboratories,Inc.Bio-RadLaboratories,Inc.ExcelMicrosoftPowerPointWindowsWindowsVistaMicrosoftCorporationAdobeReaderAdobeSystemsIncorporatedCyGEHealthcareFluorQuasarBiosearchTechnologies,Inc.SYBRTexasRedInvitrogenCorporationInvitrogenCorporationBio-RadLaboratories,Inc.PCRSYBRGreenIBio-RadCFX96CFX384Applera6,814,934B1CFX96CFX384C10006,814,934AppleraCorporationBio-Rad5'AppliedBiosystems,850LincolnCentreDrive,FosterCity,California,94404,USAEppendorfAG6,767,5127,074,367PCRBio-RadHard-Shell®EppendorfAG7,347,9776,340,5896,528,3022007246858200800840042003018019220060120927iiBio-RadBio-RadPCRPCR•()PCRPCR•LifeScienceResearch(discover.bio-rad.com)1Bio-RadCFX96CFX3842CFX96CFX384iii1.Bio-RadBio-RadLaboratoriesBio-Rad()Bio-Rad()()Bio-RadBio-Rad1-800-424-67232.XYXYCFX96CFX384iiiCFX96CFX38434CFX96CFX384C1000™C10003.4.CFX96CFX384PCRiv5CFX96CFX384ClassAFCC•IEC61010-1:2001EN61010-1:2001-1•IEC61010-2-010:2005EN61010-2-010:20032-010•IEC61010-2-081:2001+A1EN61010-2-081:2002+A12-0811•EN61326-1:2006(ClassA)EMC15.15–31oC80%2,000CFX96CFX384vBio-Radiiiiiii1...................................................11123CFXManager692CFXManager.......................................111115161820203..................................................212122232425284..................................................3131333737vi5....................................................4141454850516..................................................535354556164657...............................................69697172737578798...........................................81818587899193QC95979..............................................101101102102107109112113114115CFX96CFX384vii10.............................................121121122123124124125126127QC12812911....................................................131131133135135136138139...........................................................141viiiCFX96CFX38411CFX96CFX384••1•2•3•CFXManager6•9CFX96CFX384••USB•CFXManager™CD••CFXManagerBio-RadCFX96CFX384•100–240VAC50–60Hz•15–31oC80%•USBUSBCFX96Bio-Radiii2CFX96CFX384•CFX96CFX384•C1000™C10001.CFX96CFX96CFX38422.CFX96LEDCFX96CFX3843•••133C10003••••USBCFX96CFX384S1000™3.C1000C1000CFX96CFX384PCRCFX96CFX384CFX96CFX384C10001.C1000USB42.44.C10003.C10002C1000Bio-Rad4.C100055.CFX96C1000CFX96CFX38455.C100066.C1000246.C1000337.C10008.C100077.6CFXManager199.CFXManagerCFXManagerWindowsXPWindowsVistaPCCFX96CFX384PCRCFX96CFX3846WindowsXPWindowsVistaUSBWindows64PCCFXManagerCFX96CFX38432Windows64IntelPCCFXManager1.2.CFXManagerCDCD6.CFXManagerWindowsXPProfessionalSP2WindowsVistaHomePremiumWindowsXPProfessionalSP2WindowsVistaHomePremiumCD-ROMCD-RW10GB20GB2.0GHz2.0GHzRAM1GBRAMWindowsVista2GB2GBRAM1024x7681280x1024USBUSB2.0USB2.0InternetInternetExplorerInternetExplorerMicrosoftOfficeCFX96CFX38473.88.PDF4.Bio-RadCFXManager5.CD:\Bio-RadCFXReadme.txt136CFX96CFX384USB1.C1000USBUSB2.0A33USB2.0B2.C100083.WindowsWindowsUpdate99.4.1010.5.CFX96CFX3849CFX384384Bio-RadCFX384•HSP-3805384Hard-Shell®•HSP-3866384Hard-Shell•MSB-1001MicrosealBCFX960.2mlBio-Rad•MLL-960196•MLL-965196•HSP-9601Hard-Shell96•HSP-9655Hard-Shell96•TLS-08010.2ml8•TLS-08510.2ml8•TCS-080380.2ml•MSB-1001MicrosealB•241•0.2mlCFX9610–25μlCFX3845–20μl231011180oH3A101011.CFX961212.A10180oH3CFX96CFX384112CFXManagerCFXManager••15•16•18•20•20CFXManager•••••13•12•12•141821•15CFXManager12•1613.1414.1515.1616.CFX96CFX3841377.(.prcl)(.pltd)(.pcrd)(.mgxd)(.zpcr)TaTaCFXManager148PCRPCRPCRPCRPCRPCRPCRBio-RadPCRPCRPCRBio-RadBio-Rad8.*.pcrd69.mgxd101101167.CFX96CFX38415CFXManager1717.2137121121PCRPCR8.CFXManager16•21••69•101•121CFXManager1000•CFX96CFX384PCRC1000•C1000CFXManagerC1000PCR•96•384•48/48•C1000S1000S1000C1000CFXManagerCDPDFCFXManagerCFX96CFX384PCR17AB18•48/48C1000(C48FSIM00)•96S1000(S96FEM01)C48FSIM00C1000•CFX384(CFX384SIM03)CFX96CFX38417•CFX96(CFX96SIM02)18.••LED••••••1919.••••CFXManager18171820•C1000••20.C100018•CFX96CFX38419CFX96CFX3842121.22.CFXManager20CFXManager9CFXManager••••••••ExpressLoad.prcl.pltd••••9.CFXManager.prcl.pltd.pcrd.mgxd.zpcrCFX96CFX384213CFXManager••22•22•23•24•26•28•16•14•12•31•41•24PCR34(.pcrd)222323.23••.prcl•ExpressLoad.prcl•3060.0°CCFX96CFX384232424.2555.0°C25.26PCRCFXManager••.pltd•ExpressLoad.pltd24•26.27••27.CFX96CFX38425IDID•10••LED••CFXManager••PCR•10.......Excel...Excel.........262828.2826•••1111.CFX96CFX3842729PCR29.LED11.2829(.pltd)CFXManagerExpressLoad3030.17193131.CFX96CFX3842912•••••12.LED30•••••ExcelExcel•••CFX96CFX384314••33•37•37•22•22•2212432•••32•32.133333.13.96384CFX96CFX38433141.2.14.05096030384μl37(0)343.3634234.1.2.3441.2.963843.364.CFX96CFX384353535.1.2.352-41.2.36303636636.1.2.3.(0.00)3710oC37.HA•••CFX96CFX38437•••••1.2.24•150μl96130μl384•(0)μlPCRPCR••383838.PCR•(Ta)•(bp)PCR•DNAiTaqiProofiTag™DNAiProofTMDNA•PCRPCRPCR••••CFX96CFX38439PCR1.52.01.3-1095°C2.304015-3094-95°C15-3015-6072°C3.1072°C•395°C3098°C•3095°C192°C•2070°C23(bp