Thermo_Scientific限制性内切酶一览表

BGORT2xTFAanITTATAA37°CT50-100%50-100%0-20%0-20%100%20-50%AarICACCTGC37°CBufferAarI+oligoNRNR0-20%0-20%NR50-100%AasIGACNNNNNNGTC37°CB100%20-50%0-20%0-20%50-100%0-20%AatIIGACGTC37°CT50-100%20-50%0-20%0-20%100%20-50%FastDigest®AatIIGACGTC37°CF100%FastDigest®AccIGTMKAC37°CF100%Acc65IGGTACC37°CO0-20%20-50%100%20-50%20-50%50-100%FastDigest®Acc65IGGTACC37°CF100%FastDigest®AciICCGC37°CF100%FastDigest®AclIAACGTT37°CF100%FastDigest®AcuICTGAAG37°CF+SAM100%AdeICACNNNGTG37°CG0-20%100%20-50%100%100%20-50%FastDigest®AfeIAGCGCT37°CF100%FastDigest®AflIICTTAAG37°CF100%FastDigest®AgeIACCGGT37°CF100%AjiICACGTC37°CBufferAjiINRNR20-50%NRNR20-50%AjuIGAANNNNNNNTTGG37°CR+SAM0-20%50-100%20-50%100%50-100%50-100%FastDigest®AjuIGAANNNNNNNTTGG37°CF+SAM100%FastDigest®AleICACNNNNGTG37°CF100%AlfIGCANNNNNNTGC37°CR+SAM0-20%0-20%0-20%100%0-20%20-50%AloIGAACNNNNNNTCC37°CR0-20%0-20%0-20%100%20-50%100%AluIAGCT37°CT50-100%0-20%0-20%0-20%100%20-50%FastDigest®AluIAGCT37°CF100%Alw21IGWGCWC37°CO0-20%20-50%100%50-100%20-50%50-100%FastDigest®Alw21IGWGCWC37°CF100%Alw26IGTCTC37°CT50-100%100%0-20%0-20%100%100%FastDigest®Alw26IGTCTC37°CF100%Alw44IGTGCAC37°CT50-100%100%0-20%50-100%100%50-100%FastDigest®AlwNICAGNNNCTG37°CF100%ApaIGGGCCC37°CB100%20-50%0-20%0-20%20-50%0-20%FastDigest®ApaIGGGCCC37°CF100%FastDigest®ApaLIGTGCAC37°CF100%FastDigest®AscIGGCGCGCC37°CF100%FastDigest®AseIATTAAT37°CF100%FastDigest®AsiSIGCGATCGC37°CF100%FastDigest®AvaICYCGRG37°CF100%FastDigest®AvaIIGGWCC37°CF100%FastDigest®AvrIICCTAGG37°CF100%BamHIGGATCC37°CBufferBamHI20-50%100%20-50%50-100%100%50-100%FastDigest®BamHIGGATCC37°CF100%FastDigest®BanIGGYRCC37°CF100%BauICACGAG37°CT0-20%50-100%0-20%50-100%100%50-100%内切酶在各种Fermentas缓冲液中的活性识别位点孵育温度推荐缓冲液FastDigest®BbsIGAAGAC37°CF100%FastDigest®BbvIGCAGC37°C2xT100%BclITGATCA55°CG20-50%100%20-50%20-50%100%100%FastDigest®BclITGATCA37°CF100%BcnICCSGG37°CT20-50%50-100%50-100%50-100%100%50-100%BcuIACTAGT37°CT50-100%50-100%0-20%20-50%100%0-20%BdaITGANNNNNNTCA30°CG+SAMNR100%0-20%20-50%50-100%50-100%FastDigest®BfaICTAG37°CF100%BfiIACTGGG37°CT20-50%20-50%0-20%0-20%100%0-20%BfmICTRYAG37°CT0-20%50-100%0-20%0-20%100%20-50%BfuIGTATCC37°CBufferBfuINRNR0-20%0-20%NR50-100%BglIGCCNNNNNGGC37°CO0-20%50-100%100%100%0-20%100%FastDigest®BglIGCCNNNNNGGC37°CF100%BglIIAGATCT37°CO0-20%20-50%100%50-100%0-20%100%FastDigest®BglIIAGATCT37°CF100%FastDigest®BlpIGCTNAGC37°CF100%Bme1390ICCNGG37°CO20-50%50-100%100%50-100%50-100%50-100%FastDigest®Bme1580IGKGCMC37°CF100%FastDigest®BmtIGCTAGC37°CF100%BoxIGACNNNNGTC37°CT0-20%0-20%0-20%20-50%100%20-50%BpiIGAAGAC37°CG20-50%100%50-100%50-100%50-100%50-100%BplIGAGNNNNNCTC37°CT+SAM0-20%20-50%0-20%0-20%100%20-50%FastDigest®BplIGAGNNNNNCTC37°CF+SAM100%FastDigest®BpmICTGGAG37°CF100%Bpu10ICCTNAGC37°CBufferBpu10I0-20%20-50%50-100%100%50-100%100%FastDigest®Bpu10ICCTNAGC37°CF100%Bpu1102IGCTNAGC37°CT50-100%50-100%20-50%20-50%100%20-50%FastDigest®BsaAIYACGTR37°CF100%FastDigest®BsaBIGATNNNNATC65°CF100%FastDigest®BsaHIGRCGYC37°CF100%FastDigest®BsaJICCNNGG37°CF100%BseDICCNNGG55°CT50-100%20-50%0-20%0-20%100%50-100%BseGIGGATG55°CT20-50%50-100%20-50%20-50%100%20-50%FastDigest®BseGIGGATG37°CF100%BseJIGATNNNNATC65°CONR100%100%NRNR100%BseLICCNNNNNNNGG55°CT20-50%100%50-100%20-50%100%50-100%BseMIGCAATG55°CR0-20%20-50%0-20%100%50-100%50-100%BseMIICTCAG55°CT+SAM50-100%50-100%50-100%50-100%100%50-100%BseNIACTGG65°CB100%20-50%0-20%0-20%50-100%20-50%FastDigest®BseNIACTGG65°CF100%BseSIGKGCMC55°CG20-50%100%0-20%20-50%50-100%0-20%BseXIGCAGC65°CBufferBseXINRNRNRNRNRNRFastDigest®BseXIGCAGC65°CF100%Bsh1236ICGCG37°CR0-20%0-20%50-100%100%20-50%50-100%FastDigest®Bsh1236ICGCG37°CF100%Bsh1285ICGRYCG37°CG20-50%100%20-50%0-20%0-20%20-50%BshNIGGYRCC37°CO0-20%20-50%100%50-100%0-20%100%BshTIACCGGT37°CO0-20%20-50%100%50-100%20-50%20-50%FastDigest®BsiEICGRYCG37°CF100%FastDigest®BsiWICGTACG37°CF100%FastDigest®BslICCNNNNNNNGG37°CF100%FastDigest®BsmBICGTCTC37°CF+DTT100%FastDigest®BsmFIGGGAC37°CF+SAM100%Bsp68ITCGCGA37°CO0-20%20-50%100%50-100%20-50%50-100%Bsp119ITTCGAA37°CT20-50%0-20%0-20%0-20%100%100%FastDigest®Bsp119ITTCGAA37°CF100%Bsp120IGGGCCC37°CB100%20-50%0-20%20-50%50-100%0-20%FastDigest®Bsp120IGGGCCC37°CF100%Bsp143IGATC37°CBufferBsp143I20-50%20-50%0-20%0-20%50-100%20-50%FastDigest®Bsp1286IGDGCHC37°CF100%Bsp1407ITGTACA37°CT0-20%20-50%0-20%20-50%100%50-100%FastDigest®Bsp1407ITGTACA37°CF100%FastDigest®BspCNICTCAG37°CF+SAM100%FastDigest®BspHITCATGA37°CF100%BspLIGGNNCC37°CT50-100%50-100%0-20%20-50%100%20-50%FastDigest®BspMIACCTGC37°CF100%BspOIGCTAGC37°CO0-20%0-20%100%100%0-20%20-50%BspPIGGATC55°CT20-50%20-50%0-20%0-20%100%0-20%BspTICTTAAG37°CO0-20%0-20%100%20-50%0-20%50-100%FastDigest®BsrBICCGCTC37°CF100%FastDigest®BsrDIGCAATG55°CF100%FastDigest®BsrFIRCCGGY37°CF100%FastDigest®BssHIIGCGCGC37°CF100%Bst1107IGTATAC37°CO20-50%50-100%100%100%20-50%100%BstXICCANNNNNNTGG55°CO20-50%100%100%50-100%50-100%100%FastDigest®BstXICCANNNNNNTGG37°CF100%FastDigest®BstZ17IGTATAC37°CF100%Bsu15IATCGAT37°CT20-50%20-50%20-50%20-50%100%20-50%FastDigest®Bsu36ICCTNAGG37°CF100%BsuRIGGCC37°CR20-50%20-50%50-100%1