takara宝生物公司限制酶双酶切buffer表

EnzymeAccIBamHIBglIIClaIEcoRIEcoRVHincIIHindIIIKpnINcoINdeINotIPstIPvuISacISalISmaISpeISphIXbaIXhoISuppliedBuffer10×M10×K10×H10×M10×H10×H10×M10×M10×L10×K+BSA10×H10×H+BSA+Triton10×H10×K+BSA10×L10×H10×T+BSA10×M10×H10×M+BSA10×HAccI-0.5×K1×T1×M1×M0.5×K1×M1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×T1×T+BSA1×M0.5×K1×M1×MBamHI0.5×K-1×K1×K1×K1×K0.5×K1×K0.5×K1×K+BSA1×K0.5×K+BSA1×K1×K0.5×K1.5×T0.5×T+BSA1×K1×K0.5×K1×KBglII1×T1×K-1×H1×H1×H2×K1×K1×T1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H1×T+BSA1×H1×H2×T1×HClaI1×M1×K1×H-1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×M1×H1×H1×HEcoRI1×M1×K1×H1×H-1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×H1×H1×M1×HEcoRV0.5×K1×K1×H1×H1×H-2×T1×K0.5×K1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H0.5×K+BSA1×H1×H2×T1×HHincII1×M0.5×K2×K1×M1×M2×T-1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×K1×T+BSA1×M2×T1×M1×MHindIII1×M1×K1×K1×M1×M1×K1×M-1×M1×K+BSA1×K0.5×K+BSA1×M1×K1×M1.5×K1×T+BSA1×M1×K1×M1×MKpnI1×M0.5×K1×T1×M1×M0.5×K1×M1×M-0.5×K+BSA1×T0.5×K+BSA1×M0.5×K1×L1.5×T+BSA1×T+BSA1×M0.5×K1×M1×MNcoI1×M+BSA1×K+BSA1×K+BSA1×K+BSA1×K+BSA1×K+BSA1×M+BSA1×K+BSA0.5×K+BSA-1×K+BSA0.5×K+BSA1×K+BSA1×K+BSA0.5×K+BSA1.5×T+BSA1×T+BSA1×K+BSA1×K+BSA1×M+BSA1×K+BSANdeI1×T1×K1×H1×H1×H1×H1×T1×K1×T1×K+BSA-1×H+BSA1×H1×K1×T1×T+BSA1×H1×H1×T1×H1×HNotI0.5×K+BSA0.5×K+BSA1×H+BSA1×H+BSA1×H+BSA1×H+BSA0.5×K+BSA0.5×K+BSA0.5×K+BSA0.5×K+BSA1×H+BSA-1×H+BSA2×K+BSA0.5×K+BSA1×H+BSA0.5×T+BSA1×H+BSA1×H+BSA0.5×K+BSA1×H+BSAPstI1×M1×K1×H1×H1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA-1×K1×M1×H0.5×T+BSA1×H1×H1×M1×HPvuI0.5×K1×K1×K1×K1×K1×K0.5×K1×K0.5×K1×K+BSA1×K2×K+BSA1×K-0.5×K1.5×K+BSA1×K+BSA1×K1×K0.5×K1×KSacI1×M0.5×K0.5×K1×M1×M0.5×K1×M1×M1×L0.5×K+BSA1×T0.5×K+BSA1×M0.5×K-1.5×T+BSA1×T+BSA1×M0.5×K1×M1×MSalI1.5×T1.5×T1×H1×H1×H1×H1.5×K1.5×K1.5×T+BSA1.5×T+BSA1×H1×H+BSA1×H1.5×K+BSA1.5×T+BSA-1.5×T+BSA1×H1×H1.5×T1×HSmaI1×T+BSA0.5×T1×T+BSA1×T+BSA1×T+BSA0.5×K1×T+BSA1×T+BSA1×T+BSA1×T+BSA1×T+BSA0.5×T+BSA0.5×T1×K+BSA1×T+BSA1.5×T-1×T+BSA0.5×T1×T+BSA1×T+BSANote:1)Itisconfirmedthat10unitsofeachenzymecompletelydigest1µgofDNAat37°Cinonehourin50µlreactionmixture.2)TheconcentrationofGlycerolshouldbelessthan10%tominimizestaractivity.3)DNAmaynotbedigestedcompletely,whenrecognitionsequencesoftwoenzymesarecloseeachother,orwhenDNAtakeshigh-structureconformation.反应体系（20ul）：10*MBuffer2ul质粒T-5.3kbGt13ulXbaⅠ、HindⅢ各0.5ul（7.5U）无菌水14ul37℃水浴保温2h+BSA+BSA+BSA+BSA+BSASpeI1×M1×K1×H1×M1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA-1×H1×M1×HSphI0.5×K1×K1×H1×H1×H1×H2×T1×K0.5×K1×K+BSA1×H1×H+BSA1×H1×K0.5×K1×H0.5×T+BSA1×H-2×T1×HXbaI1×M0.5×K2×T1×M1×M2×T1×M1×M1×M1×M+BSA1×T0.5×K+BSA1×M0.5×K1×M1.5×T1×T+BSA1×M2×T-1×MXhoI1×M1×K1×H1×H1×H1×H1×M1×M1×M1×K+BSA1×H1×H+BSA1×H1×K1×M1×H1×T+BSA1×H1×H1×M-