不同生物预处理对玉米秸秆厌氧发酵产气特性的影响

2014-4-2113:21:48[URL](4):118~12220144April20141.4530002.4530003.7121004VS227.67mL·g-15.01%~81.2%VS125.64mL·g-1pHVFAS216.4X705A1005-9369201404-0118-05,,.[J].,2014,45(4):118-122.ChuLili,TianXiaoxin,YangGaihe.Effectofbiopretreatmentonbiogasproductioncharacteristicsofanaerobicfermentationofcornstalk[J].JournalofNortheastAgriculturalUniversity,2014,45(4):118-122.(inChinesewithEnglishabstract)Effectofbiopretreatmentonbiogasproductioncharacteristicsofanaerobicfermentationofcornstalk/CHULili1,TIANXiaoxin2,YANGGaihe3(1.DepartmentofLifeScienceandTechnology,XinxiangUniversity,XinxiangHenan453000,China;2.SchoolofChemistryandChemicalEngineering,XinxiangUniversity,XinxiangHenan453000,China;3.SchoolofAgronomy,NorthwestAgricultureandForestUniversity,YanglingShaanxi712100,China)Abstract:Slowdecompositionandlowrateofbiogasproductionareconstraintsthatusingstrawasanaerobicfermentationmaterials,biopretreatmentcanacceleratestrawdecompositionandincreasebiogasproductionrate.Biogasslurrywasusedtopretreatmentcornstalk,comparedwithMicrobeDecompositionAgents,EasyCompostingAgent,complexmicrobialagent,sugarfermentationenzyme,andwatertreatmentwasusedascontrol,anaerobicdigestibilityandbiogasyieldofcornstalkwereevaluatedbyanaerobicbatchdigestionexperiments.Theresultsshowedthat,pretreatmentofbiogasslurrywasbetter,thecumulativebiogasproductionof227.67mL·g-1VSwasobtainedafter43daysofdigestion,increasedby5.01%-81.2%ascomparedtootherpretreatmentmethods.Thecumulativebiogasproductionofsugarfermentationenzymewas125.64mL·g-1VS,whichwasthelowestamongallpretreatment's.Inaddition,thetotalalkalinityofbiogasslurrytreatmentwasthehighest,andhadstrongbuffercapacity,VFAdecompositionwasfast,whichledthehighestbiogasproduction.Biogasslurrypretreatmenthadasignificanteffectonincreasingbiogasproductionrateofstraw.Keywords:biopretreatment;cornstalk;biogas;anaerobicdigestion2012-12-27139902017220121224004301341981-4[1][2-8][9][10-15]15~25℃3555℃[16-18]25℃pHVFA1.11.21.31000mL25±1℃1.42cmCK0.02gA4gB8gC8gD500gE1kg7d11500g8%200g3ItemCKABCDEInoculum%Carboncontent38.7516.1042.0641.0835.2838.9635.13%Nitrogencontent0.970.981.011.331.571.121.74C/Nration40.0616.4241.5631.3122.3934.7220.23TS%14.1414.1915.3412.9913.1514.937.37%Ash7.305.867.016.687.829.9129.92VS/TS%92.7094.1492.9993.3292.1890.0970.081Table1Basiccharacteristicsofrawmaterialsandinoculum1.5①TSVS[19]TSVS②[19]K2Cr2O7③④STP⑤pHpHpHS-3CT119452.2pHpH6.5~7.52DpH6.62~7.34DpH6.706.08E1pH3EDpH52.3VFAVFA36VFAVFA6113919272719DVFA8455.50mg·L-1C5777.72mg·L-1B4887.18mg·L-1CK4221.83mg·L-1A4195.21mg·L-1E3460.25mg·L-13DVFA5⑥VFA[19]⑦[19]⑧SAS13.02.161E12317.8mL·d-15D41d247.2mL·d-14270.0mL·d-12E8204.4mL50.49%~77.09%VS1VSEABCKCD5EVS9.31%5.01%8.32%11.86%81.2%Item(mL)TotalbiogasyieldVS(mL·g-1)BiogasyieldperVSCK7723.33208.29A8164.17216.81B7818.33210.19C7597.50203.53D4632.78125.64E8204.44227.6726Table2Biogasproductionofdrymatterof6materials16Fig.1Biogasproductionrateof6materials2pHFig.2VariationsofpHCKABCDEmL·d-1BiogasproductionratedTimedTime051015202530354045300240180120600pH7.507.257.006.756.506.256.00CKABCDE812162024283236404412042.4464000mg·L-1EDE6901.60mg·L-13ED5ABCK4Fig.4Variationsoftotalalkalinity3pHVFATable3ANOVAofthepH,VFAandtotalalkalinityItempHVFAmg·L-1mg·L-1TotalalkalinityCK6.74b2567.10b4492.40dA6.84b2654.80b4733.70cB6.85b2967.90b4500.10dC6.87b3384.20b4572.80cdD6.47c4930.10a5563.40bE7.05a2430.60b6901.60a3VFAFig.3Variationsofvolatilefattyacid3.153􀏑1[20]16~42􀏑1Hansen20~30􀏑1[21]22.39􀏑163.2pH19~35pH2VFApH3VFA54VFA2􀏑1[22]VFA2􀏑12􀏑13pH[23]13.3VFAVFAVFA[24]VFA[25-27]VFAdTimemg·L-1VFA80006000400020000CKABCDE81216202428323640448121620242832364044dTimemg·L-1Totalalkalinity750070006500600055005000450040003500CKABCDE12145a.5VS227.67mL·g-15.01%~81.2%VS125.64mL·g-13VSb.pHVFAc.[1].2013[M].:,2013.[2]ZhangRuihong,ZhangZhiqin.Biogasificationofricestrawwithanaerobic-phasedsolidsdigestersystem[J].BioresourceTechnol⁃ogy,1999,68(3):235-245.[3]ChenHongzhang,Wanghui,ZhangAijun,etal.Biogasificationofsteam-explodewheatstrawbyatwo-phaseddigestionsystem[J].TransactionsoftheCSAE,2005,21(11):116-120.[4],.[M].:,2005.[5]LuoQingming,LiXiujin,ZhuBaonin,etal.Anaerobicbiogasifi⁃cati0nofNaOH—treatedcornstalk[J].TransactionsoftheCSAE,2005,21(2):111-115.[6],,,.[J].,2007,28(5):1153-1157.[7],,,.[J].,2008,24(8):189-192.[8],,,.[J].,2009,29(10):1117-1120.[9],.[J].,2007,23(3):179-283.[10].[D].:,2002.[11],,,.[J].,2005(3):33-35.[12],,,.LZF-12[J].,2013,44(8):99-104.[13],,,.[J].,2011,42(8):56-61.[14],,,.[J].,2007,13(4):583-585.[15],,,.[J].,2009,27(2):176-179.[16]IsciA,DemirerGN.Biogasproductionpotentialfromcottonwastes[J].Renewableenergy,2007,32(5):750-757.[17]RowenaT.Romano,RuihongZhang,SarahTeter,etal.TheeffectofenzymeadditiononanaerobicdigestionofJoseTallWheatGrass[J].BioresourceTechnology,2009,100:4564-4571.[18],,,.[J].,2009,25(5):172-176.[19].[M].:,1984.[20],,.[M].:,2005:1-15.[21]HansenRC,HoitinkHAJ.Poultrymanurecomposting:designguidelinesforammonia[A]