Agilent6410

WWSeminarTourSpring2006IntroducingtheAgilent6410TripleQuadrupoleMassSpectrometerCustomerPresentationSpring2006WWSeminarTourSpring20066300SeriesIonTrapLC/MS6100SeriesSingleQuadLC/MSNewSingleQuads6210Time-of-FlightLC/MS6410TripleQuadrupoleLC/MSUpdatedTOF6510QuadrupoleTime-of-FlightLC/MSNewQQQNewQTOFNewAgilent6000LC/MSSeriesAnewandexpandedportfolioforapplicationsfromlifesciencetochemicalanalysis,combiningexceptionalperformanceandlegendaryAgilentreliabilityUpdatedIonTrapFamilyWWSeminarTourSpring2006WWSeminarTourSpring2006TheAgilent6410TripleQuadLC/MS•Best-in-classsensitivity(Femtogram)withexcellentreproducibility•Multiplecostofownershipadvantages•BestPrice-Performance:30-50%Lowerpricethancomparableinstruments•Autotunethattrulyworks!Skilledandunskilledoperatorsgettopperformance•Newquantificationsoftwarehandlestaskspreviouslyconsideredun-automatable•Agilentreliabilityandease-of-useHighEndPerformancewithUnprecedentedCostofOwnershipForfoodsafety,environmental,chemical,forensics,pharmaceuticalandmetabolomicsmarketsWWSeminarTourSpring2006MRM(MultipleReactionMonitoring)170210250290210222268280165QuadMassFilter(Q3)QuadMassFilter(Q1)CollisionCellSpectrumwithbackgroundions(fromESI)Q1letsonlytargetion210passthrough190210210Collisioncellbreaksion210apart150170190210210158191Q3monitorsonlycharacteristicfragments158and191fromion210forquantandqual.160158190191nochemicalbackgroundWWSeminarTourSpring2006AgilentTripleQuadandQTOFDesignsResultofLong-StandingInnovationIonPulserTurbo2Octopole1DCQuadRoughPumpTurbo1Turbo1Turbo1QuadMassFilter(Q1)CollisionCellLens1and2Octopole2RoughPumpOctopole1Turbo1Turbo1Turbo1QuadMassFilter(Q1)CollisionCellLens1and2QuadMassFilter(Q3)10KVDynodeDetectorTripleQuadAnalyzerQTOFAnalyzerOver10YearsinLC/MSSamplingandPatentedOrthogonalGeometryresultsinindustryleadingsensitivityandrobustnessWWSeminarTourSpring2006++++++++++++++++++++++HeatednitrogendryinggasDielectriccapillaryentranceNebulizertipaimedat90degreeangletoinletofcapillarySolventsprayIonsOrthogonalIntroductionandElectrosprayIonizationWWSeminarTourSpring2006Agilent6410AnalyzeraQuadMassFilter(Q3)HEDDetectorRoughPumpTurboPumpTurboPumpTurboPumpQuadMassFilter(Q1)CollisionCellLens1and2UtilizeasinglethreestageturbopumpWWSeminarTourSpring2006InnovationsinFront-EndIonOpticsDeliverBetterSensitivityAcrossaBroadMassRangeKeycomponentscontributingtosensitivity•Dielectriccapillary•Smalldiameteroctopoleionguide•HighfrequencyRFoctopole•Lens2RF(transmissionofhighermasses)•Hyperbolicpost-filterandquadrupoleOctopole1RoughPumpTurboTurboQuadMassFilterLens1and2(Q1)CapillaryVacuumwallWWSeminarTourSpring2006CapillarySkimmerOctopoleionguideIonpathfromcapillarythroughskimmerandintoOctopoleIonGuideWWSeminarTourSpring2006InnovationsinCollisionCellTechnologyProduceBetterSensitivityandFasterMS/MSExperimentsNomemoryorcross-talkeffects1MS,5MS/MSscans/secindatadependentmodeContributingcomponents:ƒSmalldiameterhexapolecollisioncellƒHighfrequencyhexapolecollisioncellƒLinearaxialaccelerationƒHighpressurecollisioncell:NitrogengasƒHighspeeddigitalelectronicsTurboCollisionCellWWSeminarTourSpring20061stQuadrupoleBeamshaperHEXAPOLERODSEntrancelensExitlensBeamshaper3rdQuadrupoleAgilent6410CollisionCellWWSeminarTourSpring2006CrosstalkEvaluation500pgAlprazolam,20msdwelltime00.511.522.533.544.555.566.577.58Abundancevs.AcquisitionTime(Min)0.511.522.533.544.555.566.577.588.59+EIC(281.0m/z)MRM(400.0m/z);fromalprazolam20DwellCrossTalk500pgOC-101705-28V_5uL.d11AreaRSD=0.68%=Nodetectablecross-talkm/z400→m/z281Topscaleis225Xbottom1800cts→8cts→m/z309→m/z281WWSeminarTourSpring2006Dwelltimeevaluation200pgAlprazolam100,20,5msdwelltimesDwell100ms20ms5msArea148601360513202%RSD0.610.742.255msdwellWWSeminarTourSpring2006BeamTurn-offCharacteristics10100100010000100000-5000500100015002000microsecondsArb.Unitsmz922mz118CollisionCellClearingProfileCollisiongas=7mTorr0Vcollisionenergy5VAppliedAxialPotential600μsec350μsecWWSeminarTourSpring2006Collisioncell(hexapole)RearQuadrupoleDetectorBeamshaperExitlensFromcollisioncellthrough2ndquadrupoleandiondetectionWWSeminarTourSpring2006ReserpineStandardsSensitivityinjectedoncolumn100pg500pg50pg10pg5pg1pg0.5pg0.1pgBlank1pg0.5pg0.1pgBlankWWSeminarTourSpring2006ReserpineinDirtyMatrix(3MRMs)(on-columninjection)10pg5pg2.5pg1.25pg0.63pg0.32pg0.16pgBlank1.25pg0.63pg0.32pgBlank0.16pg20pgWWSeminarTourSpring2006AlprazolamCalibrationResults(LP5)1pg–10ng,PositiveESIMode1pg5pg10pg5ng10ng2ng1ng0100000200000300000400000500000600000700000800000900000010002000300040005000600070008000900010000AbundanceAmount(pg)AlprazolamLinearityY=77.2x+1593.4R2=0.9974WWSeminarTourSpring20061pgBentazone(herbicide)injectedon-column2x102Abundancevs.AcquisitionTime(Min)0.511.522.533.544.555.566.577.588.59-TICMRM(238.9m/z);frombent_041105_1ppb.d111x1001-EIC(238.9m/z)MRM(238.9m/z);frombent_041105_1ppb.d112x1012-EIC(132.0m/z)MRM(238.9m/z);frombent_041105_1ppb.d6.5762.4897.9315.2143.8541.122111x10024-EIC(196.8m/z)MRM(238.9m/z);frombent_041105_1ppb.d11PeakNumberCenterXAreaAreaPercentAreaSumPercentBasePeakMassHei