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Aquaculture水产养殖Genetics遗传学Genomics基因组学Teleosts硬骨鱼类Shellfish贝类Shrimp虾Physiologic生理的Mechanisms机制Maturity成熟Bodyweight体重Ovary卵巢Testis精巢Dimorphism二态性Hormone激素disulfidebonds二硫键mediate介导receptor受体reproduction繁殖immunity免疫endocrine内分泌的promotion促进clone克隆signaltransduction信号转导Transformation转化Transfection转染Infection感Characterize鉴定Gene基因Pool池塘Photoperiod光周期watertemperature水温reproductivemanipulation繁殖调控egg卵sperm精子semen精液female雌鱼male雄fertilization受精volume体积hatch孵化gentleaeration微充气larvae仔鱼triplicate重复3次dose剂量Gonad性腺RNAextractionRNA提取Ethanol酒精Sex性别Blood血Brain脑Eye眼Gill鳃Kidney肾Intestine肠Liver肝Muscle肌肉Pituitary垂体Skin皮肤Spleen脾脏Stomach胃Microscope显微镜Aliquot份Activate激活Digestion消化Protocol实验计划Primer引物Polymerase聚合酶Reversetranscription反转录Sequence序列Fragment片段Amplification扩增Template模板Cycle循环Intron内含子Exon外显子Vertebrate脊椎动物Kit试剂盒Instruction用法说明Agarosegel琼脂糖胶Band带Propagate繁殖aminoacid氨基酸alignment比对phylogenetictrees进化树marker标记Quantitative定量的Stage时期Procedure程序Software软件Concentration浓度Motility活力encodingregion编码区signal信号residue残基flounder牙鲆commoncarp鲤鱼grasscarp草鱼tissue组织population群体sexratio性比negative负的positive正的control对照grow生长production生产molecular分子的stock群体sexdeterminationmechanisms性别决定机制superfemale超雌个体progeny后代feeding喂养manipulation管理environment环境study研究species种类catfish鲶鱼microsatellite微卫星elucidate阐明furtherinvestigations进一步的研究significantly显著地spawn产卵expression表达up-regulate上调fold倍gynogenesis雌核发育homozygosity纯合性meiotic减数的irradiate灭活heterologous异源的coldshock冷休克diploid二倍体的morphology形态homogamete同配marine海洋flatfish比目鱼coastalareas沿海地区adult成体individual个体investment投资chromosome染色体approach方法monosexual单性的secondmeioticdivision第二次减数分裂induce诱导mitogynogenesis卵裂雌核发育thefirstmitoticdivision第一有丝分裂homologous同源的development发育paternal父母的offspring子代Nowadays当前Cryopreserve冷冻Technique技术Practical实际的Feasible可行的Neomal伪雄鱼Theoretical理论的geneticdiversity遗传多样性sexcontrol性别控制lab实验室sexdifferentiation性分化heterozygosity杂合度recombination重组locus位点Additionally另外地Increment增量cultivatedpopulation养殖群体basepairs碱基对feasibility可行性parameter参数survivalrate成活率gynogeneticpopulation雌核发育群体thaw解冻fisheries水产trial实验experiment实验storage保存liquidnitrogen液氮waterbath水浴appropriate适宜的condition条件haploid单倍体diploid二倍体hybrid杂交子treatment处理incubator培养容器percentage百分率embryo胚胎fertilizationrate受精率survivalrate成活率data数据batch批randomly随机地sample取样classic经典的mixture混合物enzyme酶Ligation连接Restriction限制性的Dilute稀释pre-amplification预扩automatic自动的denaturation变性anneal退火elongation延伸polyacrylamidegel聚丙烯酰胺胶silverstaining银染molecularweight分子量allele等位基因index指数Initiation起始Duration持续Putative推定的Polymorphism多态性linkagedisequilibrium连锁不平衡artificial人工的diploidization二倍化optimization优化proportion比例sexreversal性逆转crossover交换incorporation整合eel鳗鲡summary摘要abstract摘要discussion讨论references参考文献materialandmethod材料和方法result结果title题目manuscript草稿revise修改review评审funding资助geneticsexing遗传性别鉴定Acknowledgments致谢Mechanism机制Article文章Foundation基础Methylation甲基化Aromatase芳香化酶Promoter启动子Androgen雄激素Estrogen雌激素Masculinization雄性化inverserelationship负相关suppress抑制genotypicsexdetermination遗传性别决定temperature-dependentsexdetermination温度性别决定thermosensitiveperiod温度敏感期non-mammalianvertebrates非哺乳类脊椎动物sexsteroid性类固醇激素hypothesize假定pattern模式pathway通路epigenetic表观遗传的nuclear细胞核transcriptionfactors转录因子biosynthesis生物合成mutual共同的dinucleotides二核苷酸transcriptionstartsite转录起始位点overall总体上frequencies频率phenotype表型genotype基因型housekeepinggene持家基因exogenous外源的temperature-dependent温度依赖的sex-dependent性别依赖的rear养殖culture养殖stimulate刺激block阻止bioinformatic生物信息的putative推定的co-transfection共转染transcriptionalactivators转录激活因子luciferasereporterassay荧光素酶报告基因检测openingreadingframe开放阅读框similarity相似度development发育demethylation去甲基化process过程sexdifferentiation性分化silence沉默DNAmethyltransferasesDNA甲基转移酶Identify鉴定、发现Influence影响Consistent一致的Nutrition营养Disease疾病Ingredient成分bodysize体长polygenic多基因的monosexpopulations单性群体determine确定match匹配consequence结果migration迁移discern辨别hypermethylate超甲基化phenomenon现象conserved保守的threshold阈值scheme计划FertlizatingrateandsurvialratearetheimportantparametersofrevaluatingThequalityofeggs受精率和成活率是评价卵的重要参数Forimprovingtheculturingproduction,feedingandmanagentisveryimportant饲养的管理对于提高养殖产量非常重要的Thefirststepofbreedingprojectdesignistheselectionofthebasepopulation基础群的选择是育种计划设计的第一步Thetilapiahaveauniquebreedingcharacteristics:Maleterritoryestoblisherandfemalemouthbrooders罗非鱼具有奇特的繁殖习性:雄性领地占领者和雌性口腔孵化者Thehalf-smoothtonguesoleshowedsignificandgrowthdifference半滑舌鳎两性之间生长差异显著Ihavetenpoolsprovidingforculturingtilapia我有十个池塘养殖罗非鱼Reproductivemanipulationisofimportanceforgonadmaturity繁殖调控对鱼类的性腺成熟时非常重要的Thehatchingstageofsoleeggsis3days舌鳎卵需要三天才能孵化Nowadaystherearealotofofniletilapiafemiliesallovertheworld目前世界上有很多罗非鱼的品系Gettingreadywithdata:gatherallimportantdata,analyses,plotsandtables,organizeresultssothattheyfollowalogicalsequence,consolidatedataplotandcreatefigureforthemanuscript,discussthedatawithyouradvisorandnotedownimportantpointsFirstdraft:identifytoworthreeimportantfindingsemergingfromtheexperimentsmakethemthecentralthemeofthearticle,notegoodorbadwritingstylesintheliterature,someasimpleandeasytofollowsomearejusttoocomplex,notethereadershipofthejourn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