lateral-flow-assay侧向层析检测资料

1GEHealthcare2GEHealthcare3GEHealthcareChangingTheParadigmEarlier,MoreAccurateDiagnosisTrackefficacyoftreatmentEarlier,MoreTargetedTreatmentCriticalinformationwheneverandwhereverit’sneededIdentifyAtRiskPatientsEarlierFrom“LateDisease”To“EarlyHealth”4GEHealthcareGEgrowth…ourinnovationGlobalResearchCenterHeadquartersNiskayuna,NewYorkJohnF.WelchTechnologyCentreBangalore,IndiaGlobalResearch—EuropeMunich,GermanyChinaTechnologyCenterShanghai,China5GEHealthcareDiagnosticsProducts&Applications诊断产品&应用FlowThroughAssay渗滤法•Membranes膜•Absorbents吸收垫DipstickAssay试纸检测法•CelluloseFiber纤维素SamplePreparation样本制备•GlassFiber玻璃纤维•TrackEtchedMembranes径迹蚀刻膜•CastMembranes浇注膜FlowThroughLateralFlowLateralFlowAssay侧向层析法SampleWicking样品吸收ConjugateRelease结合物物释放Membranes膜Absorbents吸收垫ContentsPARTI内容——第一部分DiagnosticstestsystemsKeypartsofalateralflowtestSuggestedrangeofproductssuitablefordiagnosticsSamplewicksBloodseparation&otherbodyfluidsConjugatereleaseAbsorbent&dipstickmaterialsWhydoesqualitymatter?诊断检测系统侧向层析检测的主要部件适用于诊断检测的产品样品垫全血及其他体液的分离结合物释放吸收垫及试纸条的材料为什么质量很重要？8GEHealthcareDiagnosticsTestSystems诊断检测系统EndUsersandProfessionalUsers非专业及专业用户LateralFlowAssay侧向层析检测SampleWicking样品吸收ConjugateRelease结合物释放Membranes膜Absorbents吸收垫‰Rapiddiagnosticsofpregnancy,drugs,cardiacmarkers,swineflue,HIV,etc.‰怀孕、毒品、心肌标志物、猪流感、艾滋病等的快速诊断。9GEHealthcareDiagnosticsTestSystems诊断检测系统‰Membrane-basedimmunochromatographicassayLateralFlowAssay侧向层析检测SampleWicking样品吸收ConjugateRelease结合物释放Membranes膜Absorbents吸收垫‰基于膜的免疫层析检测图1：典型的侧向层析检测条。1=样品垫，2=结合物释放垫，3=检测线，4=反应膜，5=对照线，6=吸收垫，7=背衬。10GEHealthcareTestLine检测线ParticleConjugate结合物Wick吸收垫SamplePad样品垫NitrocelluloseMembrane硝酸纤维素膜ControlLine对照线LateralFlowTest侧向层析检测11GEHealthcareDiagnosticsPrinciple诊断原理图2：由于夹心法侧向层析测试需要检测物至少提供两个抗原表位，因此通常只用于大分子待测物的检测。阳性检测会显示两条线，而阴性检测只出现一条线（对照线）。竞争法检测用于不具备有两个独立的抗体结合位点的小分子待测物的检测。在这种检测中，用于捕获待测物的抗体被固定化的竞争剂（待检测物质的类似物）取代。当样品中存在待检测分子，识别抗体的结合位点被这些分子占据，这使得结合物与检测线的结合不复存在（如待分析物质和竞争剂争夺吸附结合物）。1=大分子待检测物，2=结合物，3=捕获抗体（根据分析物情况而定），4=对照线抗体（如anti-IgG），5=小分子待测物，6=竞争剂。12GEHealthcareDiagnosticsTestSystems诊断检测系统‰Rapiddiagnostics(3to5min)ofe.g.antibodies(serology),etc.ProfessionalusersProfessionalUsers专业用户FlowThroughAssay渗滤检测Membranes膜Absorbents吸收垫‰抗体（血清学）等的快速检测（3到5分钟）。适合专业用户使用图23：典型的渗滤法装置的组成。1=检测盒。2=反应膜。3=吸收垫。4=封闭试剂。13GEHealthcareDiagnosticsTestSystems诊断检测系统LineAssay谱线分析WesternBlots蛋白印迹Membranes膜‰Membranestripswithantigenorantibodylinestodetectautoimmundiseasesetc.Oftenusedas“homebrew”-testmadebydiagnosticlabsProfessionalUsers专业用户‰带有抗原线或抗体线的膜条可用于检测自身免疫性疾病等。通常由诊断实验室自制使用。14GEHealthcareDiagnosticsTestSystems诊断检测系统DipstickAssay试纸检测CelluloseFiber纤维素‰Easy“dip-intobody-fluid”–“colour-reaction”tests.E.g.Urinedip-sticks(Roche)ProfessionalUsers专业用户‰简易的试纸-比色法检测如：尿试纸（罗氏）15GEHealthcareDiagnosticsTestSystem诊断检测系统FlowThroughLateralFlowSamplePreparation样品制备GlassFiber玻璃纤维TEM径迹蚀刻膜CastMembranes浇注膜‰Sampleprepbyfiltration.Capturingofcellsonmembranesurface(TEM),removalofbloodcells,etc.‰通过渗流制备样品在膜表面捕获细胞(径迹蚀刻膜)、去除血细胞等。ContentsPARTI内容——第一部分MembranesinlateralflowtestsKeyPropertiesSuggestedrangeofproductsWhydoesqualitymatter?侧向层析检测中的膜主要特性产品的归类为什么质量很重要？KeyPartsofaRapidTest(Focus:LateralFlowTest)快速检测的重要成员（重点关注：侧向层析检测）SamplePreparationConjugateReleaseMembranesAbsorbentsPARTII第二部分NitrocelluloseMembranesasthereactionpartofatest作为检测中反应部件的硝酸纤维素膜样品制备结合物释放膜吸收垫18GEHealthcareMembranesinLateralFlowAssays–KeyProperties侧向层析检测中的膜——主要特性ThemembraneisamatrixwhichcarriesandpreservesatleasttwodifferentcapturereagentsItmustallowthemixingofmaterialsandtheirinteractionwiththecapturelinesItmustallowfluidflow至少结合两种不同的捕获试剂必须在捕获线上能使材料和与之作用的物质混合必须能使液体流过19GEHealthcareMembranesinLateralFlowAssays-Structure侧向层析检测中的膜——结构MembranesarebeingmadebyarandomprecipitationofNCfibresfromasolutionBestcomparison:Asponge膜由溶液中的硝酸纤维随机沉淀而来最好的比喻：海绵20GEHealthcareStructureofNitrocellulose硝酸纤维的结构ScanningElectronMicrographofaWhatmanAE100membrane.Nominalporesize12μm.WhatmanAE100膜的电镜扫描图。标称孔径12μm。21GEHealthcareAirSideandBeltSideofNitrocelluloseMembranes硝酸纤维素膜的气面和带面WhatmanAE100膜在500倍放大倍数下的SEM图像。左图：空气面。右图：皮带面。这种非对称性在大孔径膜上表现的更加明显，如AE100，其标称孔径为12μm。22GEHealthcareCharacterisationofMembranes膜的特性Twoparametersareusedintheliterature:•Poresize•Capillaryrisetime文献中使用的两个参数：•孔径•毛细上升时间23GEHealthcareDistancetravelledagainsttime随时间迁移的距离24GEHealthcareDistancetravelledagainsttime随时间迁移的距离25GEHealthcareProblemswithporesizedefinition孔径精确度的问题012345051015Nominalporesize(microns)Measuredporesize(microns)26GEHealthcareCorrelationbetweennominalporesizeandcapillaryrisetimeforWhatmanmembranesWhatman膜的标称孔径及毛细上升时间之间的相关性Nominalporesize(μm)Timetowick4cm(sec)3245518581401210027GEHealthcareChangeofCapillaryRiseRateDependingonMigrationDistance毛细上升率随迁移距离的改变情况28GEHealthcareEffectofwickingrateonapparentanalyteconcentration浸润率对被测物表观浓度的作用Distanceofcapturelinefromorigin(cm)Lateralwickingrate(cm/15secs)Apparentconcentrationofanalyte21.912.50.77.430.34029GEHealthcareAssaysensitivitydecreaseswithincreasingcapillaryflowrate检测灵敏度随着毛细上升率的升高而降低30GEHealthcare10012014016018020035812Membraneporesize(micron)RelativeIntensityTypeATypeBTypeCLevelofbindingobservedond