曼氏无针乌贼胚胎发育与人工育苗技术的研究

3:1008－830X(2009)03－0257－073160042003、、。20066810585586.0%20mm418271.4%92～130mm106～219g288569.0%1000151.6kg97.02。S917.4AStudyonEmbryoDevelopmentandArtificialBreedingofSepiellamaindroniCHANGKang-mei,WUChang-wen,LVZhen-ming,etalMarineScienceSchoolofZhejiangOceanUniversity,KeyLaboratoryofZhejiangProvincialMarineCulturingEquipmentsandEngineeringTechnology,Zhoushan316004,ChinaAbstract:Tosolvethekeytechniquesoffetation,seedlingbreedingandadultculturinginartificialprop－agation,thereproductivebiologyofSepiellamaindroniwasstudiedfrom2003.Theresultsprovidedthetechni－calsupportforsolvingtheindustrializationdevelopmentofS.maindroniandrestorationoffisheryresourcesinZhoushanfishingground.6810fertilizedeggsofS.maindroni,whichwerefertilizedbythethirdgenerationandthewildcuttlefishwerecapturedatDongjiseaareain2006.5855juvenilswereachievedwithahatchingratewas86.0%,4182juvenilswithcarcasslength20mmwereachievedwithasurviveratewas71.4%,2885adultcuttlefisheswithcarcasslength92-130mmandweight106-219gwereachievedwithasurviveratewas69.0%.Underartificialfarmingandpropagationcontrolling,1000culturedcuttlefisheslay151.6kg,970.200fertilizedeggswereachievedinautumn.Keywords:Sepiellamaindroni;fetation;seedlingbreeding;adultculture;artificialpropagationSepiellamaindronideRochebrune、、(()JournalofZhejiangOceanUniversity(NaturalScience)28320099Vol．28No．3Sep．,[e-mail]()28)。、、。“”。。、[1-3]。“”6t9.3%。2070、“”、、[1-5]。19822003、、、。2006。11.12006656810。0.6m3。1.2600m353.0m×3.0m×1.4m。84.0m×4.0m×1.4m。24h。、、、、、。16.2～28.0℃25～28pH8.0～8.25mg/L0.1mg/L500～800lx。1.3180%～100%。24h。23.5～24.2℃[2]18d。1.41000～2000/m3。24h。---1。5～10/mL3～7/2～5/,2～5/2～4/。20mm。3、1[24]。1Tab.1ThebaitsseriesofSepiellamaindron1d3d6d9d12d15d18d21d24d27d30d25831100%～120%。4～6d1。24h。1.520mm300～500/m3100mm40～50/m3。、、、、、、。8～12。21。1120%～150%14～6d1。24h。1。1.630～40/m3。、、、、。3～4cm。22.12.1.123.5～24.2℃、、、、、、、18d12。1-A。7.5～15.0mm×6.5～9.0mm3.0～3.6mm×2.3～2.9mm0.15g。。31-B。。10h10min29h35min1-C～G。60h50min1-H65h05min70h30min1-I81h55min105h55min6d0.8～1.0mm1-J7d1/48d1/29d3/41-K10d1-L。、、、、。2-M～S11d2-T～U。12d13d2-V。15d2-W18d2-X、Y。4.8～6.0mm10.25mm。0.86mm0.64mm。30min259()28。480～500h·℃。14.95～6.10mm。35.80～7.10mm34～36h。。56.25～7.30mmU。。。8～1510.50～13.97mm。18～2015.10～20.31mm。2.1.26568106585586.0%。7820mm418271.4%。1085～130mm87～219g288569.0%。10～12100056633412～15cm13.5cm120～170g145g。2006151.6kg97.02。87g90g。2500h·℃。33.1。4～6122°30′～122°50′E30°07′～30°17′N。2003-2006、、—700020066810[7-10]。3.2102345。2006928℃24℃200～300lx15d2500h·℃。102～1221000566、434151.6kg97.021714。[3,7]。3.3、、、、。、、、。、。。、[11]。2603[1],,.Sepiaandreana[J].,2006,373231-237.[2],,,.[J].,1984,32131-140.[3],,.[J].,1986,52147-154.[4],,.[J].,1986,52155-164.[5],,.[J].,1986,52171-176.[6],.SepiellamaindronideRochebrune[J].,1982,111-10.[7],,.SepiellamaindronideRochebrune[J].,1985,411-8.[8],,.[J].,1986,5299-104.[9],.[J].,1986,52165-170.[10],,.[J].,1986,52177-182.[11],,,.(Sepiellamaindroni)[J].,2008,38855-56.261()281Plate1×40A.B.C.2D.4E.8F.16G.32H.I.J.K.3/4L..A.fertilizedeggsB.fertilizedeggswithouteggmembraneC.2-cellstageD.4-cellstageE.8-cellstageF.16-cellstageG.32-cellstageH.blastocyststageI.gastrulastageJ.blastodermepibolyK.blastodermepiboly3/4L.blastodermepibolyfinished.26232Plate2×40M.O.P.Q-R.S.T-U.()V.W.X.,×20Y.,×20.M.imaginaldiscscomeintobeingO.imaginaldiscsformationstageP.embryoniceminentiaQ-R.organsshapedS.organsbasicallyformedT-U.cross-seedlingstage(Red-beadstage)V.endoskeletonformationstageW.heartpulsationstageX.youngcuttlefishineggmembraneY.youngcuttlefish.263