PDF下载-聚中性红作电子转移介体的过氧化氢生物传感器

3(,250014),,H2O2,:2.010-52.510-2molPL;1.810-5molPL,95%20s,,,,2001211225;20022032021,1,,,,2,3,4,,,,5(NR)6,(PNR),22.1LK298(),(HRP,,R.Z.-3.0),2.2(HPBS)2.2.16,:01025molPLPBS(pH=6)+0.5molPLNaNO3+3.010-4molPLNR(-1.00.6V)CV,CV,,,PNR6PNR50mVPs,(EP1EP2)60mV,ipaPipc1,2.2.2(HRP):10L40gPLHRPpH7.0PBSPNR,,,,10L5%(37),5%1min,,2.3,,30200210(FENXIHUAXUE)ChineseJournalofAnalyticalChemistry1012371239H2O2,33.1:,,1h,PNR,,,,,,,,;,,,1HPBSCVFig.1Thecyclicvoltammetric(CV)curvesofhydrogenperoxidasebiosensor(HPBS)atdiffrentscanrates(medium):K2HPO42KH2PO4(PBS)(pH=7);v:50100150mVPs(frominnertoouter)3.2HPBSpH7.010,2,,,PNR,,CV,(1)ipaipc,3.3H2O22H2O2H2O2,NR(a),H2O2,NR(b):,,NR,,H2O2,HRPH2O2,NR:H2O2+2H++HRPredHRPox+2H2OHRPox+NRredHRPred+NRoxNRox+e+H+NRredH2O2,H2O23.43H2O2-(-0.5V)H2O2,2.010-52.510-2molPL;r0.992;1.810-5molPL3.5,(3.5mm)10L,3,,95%20s3.6,2mmolPLH2O210,RSD2.0%,8321302HPBSH2O2Fig.2TheCVcurvesofHPBSwith(-)andwithout(---)H2O2(medium):K2HPO42KH2PO4(pH=7);v:100mVPs;a.H2O2=0;b.H2O2=5mmolPL3H2O22Fig.3Current2timecurveofthesensor(medium):K2HPO42KH2PO4(pH=7);v:100mVPs;E:-0.5V;H2O2=1.02.05.08.010.0mmolPL(fromuptodown)14,44,90%3.7-0.3V-0.7V,,,,-0.5V3.8pHpH6.57.5,pH7.0,References1LiQingshan,YeBangce.FreseniusJ.Anal.Chem.,1999,363:2462502KhanGF,WernetW.Anal.Chem.,1997,69:268226873TianChengyun(),CaoDajun().ChineseJ.Anal.Chem.,(),1998,26(7):8548574WangJ,WuL,LuZ,LiR.J.SanchezAnal.Chem.Acta,1990,228:2515ZhuMin,HanShubo.J.Electroanal.Chem.,2000,480:2552616SunYuanxi(),YeBaoxian().ChineseJ.Anal.Chem.(),1998,26(7):166169AHydrogenPeroxideSensorModifiedbyPoly(neutral2red)astheMediatorZhangGuorong3,LiuHaiyan,WangYanling(DepartmentofChemistry,ShandongNormalUniversity,Jinan250014)AbstractAparaffinsoakedgraphiteelectrodewasfabricatedbypoly(neutralred)film,coveringwithalayerofgelatin,andfinallycross2linkingwithglutaraldehyde.Themethodgreatlyshortentherunning2offofwater2solublemediator,nutralred,andimprovedthestability.ThefabricatedH2O2sensorpresentsalinearrangefrom0.02mmolPLto25mmolPLH2O2;Theresponsetimeandthedetectionlimitwere30s,0.018mmolPLrespectively.Thesensorretainsmorethan90%ofinitialresponseafterstorage(4)forfourmonths.KeywordsPoly(neutral2red),gelatin,hydrogenperoxidase,biosensor,paraffinsoakedgraphiteelectrode(Received25November2001;accepted2March2002)932110: