尼古丁印迹聚合物分子识别特性的光谱研究07

SpectroscopyStudiesonMolecularRecognitionofaMolecularlyImprintedNicotine-SpecificPolymer1221(11230052;21233010)[],,,1H,12;[][]O65713[]A:(),,,,,[1](NIC),90%,[2][3],,[4],,(MAA),1H(NMR),NICMAANIC,11.1(Sigma);(Fluka);();UV-2401PC(Shimadzu);AVANCEAV400(Bruker);SHZ-82()1.21mmol,4mmol,20mmol,0124mmol516mL,5min,,6024h,2536Lm,300mLö91(vv)24h,1.3,012mmolöL1cm,,0121186220071mmolöL,5min,1.41H,(TMS),2520mmolöL,40mmolöL,(20mmolöL)(40mmolöL)1H22.1(NIC)(MAA)MAA,NICMAA,,,1P-P3[5]NICa0,MAAb0,n,K,:NIC+nMAA=[NIC][MAA]nK=[[NIC][MAA]n]ö[NIC][MAA]n(1),[NIC]+[[NIC][MAA]n]=a0(2)[MAA]+n[[NIC][MAA]n]=b0(3)b0a0,(3)[[NIC][MAA]n],[MAA]=b0(4)(2)(4)(1),[[NIC][MAA]n]=a0b0nKö(1+b0nK)(5)Lamber-Beer,E1E2NICNIC-MAA,:A=E1l[NIC]+E2l[[NIC][MAA]n]=E1l(a0-[[NIC][MAA]n])+E2l[[NIC][MAA]n]=E1la0+(E2-E1)l[[NIC][MAA]n](6)NIC,$A:$A=A-A0=(E2-E1)l[[NIC][MAA]n]=$E2l[[NIC][MAA]n](7)(7)(5),$Aöb0n=-K$A+K$E21a0(8)(8)n,,n=123,$Aöb0n$A,n=1n=3,n=2,$Aöb0n=11173-61861$A,r=019983NICMAA12,K=61861106L2mol-2,NICMAA,1(25)a0=0120mmolöL;b0ö(mmolöL):a2010;b20120;c20140;d20180;e21120;f2118012.21H1H,22(b)1HNMR,11126ppm2(c),12,1HNMR12118ppm,,,[6]2(a)(b)-(c)1HNMR(25)372,,,1H,121H,;33,[1]1[J]1,2005,17(3):531[2]1[J]1,1998,(2):43[3]Doctor,P.B.Determinationofnicotineandcutinizeintobaccoharvestersurinebysolid2phaseextractionandliquidchromatography[J]1J1Chromatogr1B,2004,802(2):323[4]1[J]1,2003,31(10):1202[5]1[J]1,2003,31(10):1173[6]Matsui,J.Amolecularlyimprintedsyntheticpolymerreceptorselectiveforatropine[J].Anal.Chem.,1995,67(23):44048220071