大蒜主要活性成分及药理作用研究进展_马丽娜

ＲoleofmitochondrialcalpainsinapoptosisSONGBi-weiFANQiao-meiHEZhi-yuCollegeofParmaceulticalScienceZhejiangUniversityofTechnologyHangzhou310014ChinaAbstractCalpainsarethefamilyofCa2+-activatedcysteinepro-teases．Althoughcalpainsareconsideredtobecytoplasmicen-zymesrecentresearchhasdemonstratedthatμ-calpainm-cal-paincalpain10andtheirendogenousinhibitorcalpastatinarepresentinthemitochondriaandplayimportantrolesbothincaspase-dependentand-independentpathwaysincelldeathphe-nomena．CalpainsexertdirectandindirecteffectsonthecaspasesandregulatorsofapoptosispathwaysuchasBcl-2BaxBidpromotingthereleaseofCyt-CAIFthenresultincellularap-optosis．Toallowpharmacologicaltargetingoftheseenzymesthoroughknowledgeoftheirpatternsofactivationandfurtherin-teractionswithalreadyknownapoptoticpathwaysisnecessary．KeywordsmitochondriaCa2+μ-calpainm-calpaincal-pain10apoptosis．2014－5－211050http//．cnki．net/kcms/doi/10．3969/j．issn．1001－1978．2014．06．006．html122321．1000292．1000913．830054doi10．3969/j．issn．1001－1978．2014．06．006A1001－1978201406－0760－04Ｒ-05Ｒ282.71Ｒ284.1Ｒ972Ｒ979.1AlliumsativumL．、。。、《》、《》AlliumsativumL．。。《》“”“、、、”。、[e-mail][e-mail]。。1。alliinS-S-allylcysteinesulfoxide。allinase5’-44851500u135℃。AllicindiallylthiosulfinateFig1。DADS、DAS、ajoeneDATS2Fig2。、、、、。Fig1Allinasecatalyzealliintoproduceallicin32·067·ChinesePharmacologicalBulletin2014Jun306760～32．12．1．1、、、。Sangeetha4－80.04、0.08mg·g－1、CK、CK-MB、TC、TG、ICDH、SDH、α-α-KG-DH、NADH、C、Na+K+-ATP、Ca2+Mg2+-ATP。2．1．2。9+MDA、TC、LDL-CSODHDL-C0.2mg·g－1TCSOD0.04mg·g－1。、3--3-AHMG-CoA。100.08mg·g－1。DADS、11、、。2．1．3、、。Hui1210mg·L－130minTNF-αTNF-αVCAM-1mＲNANADHNOX4JNK、EKＲ1/2、IKBαTNF-α。、·O－2·OH13。Nrf214。Li150.18mg·g－1Nrf2。Louis16、NOH2S。、、。2．2。17+2∶137℃13h5、30、50、80、100150mg·L－1。+MGC-803HeLaMGC-803S。SGC-7901DADS18－19。Chu20。10mg·L－1Nrf221。。2．3Fig2Allicindecomposesintoaseriesofsulfur-containingcompounds3·167·ChinesePharmacologicalBulletin2014Jun306、。。B、、。22。33．119752010All-trideC6H10S3。23－25。3．2、、、、、、。3．3、、、、。、。。。。4“”。、、。、。26－27、、、、、+。1000IU90%～95%。28－29HPLC30。、、。1KuettnerEBHilgenfeldＲWeissMS．Purificationcharacteriza-tionandcrystallizationofalliinasefromgarlicJ．ArchBiochemBiophys20024022192－200．2BlockE．ThechemistryofgarlicandonionsJ．SciAm19852523114－9．3．J．2011311180－5．3WeiNQWuJMZhangQM．DiscussionondrugspecificationofgarlicandalltrideJ．ChinJPharmAnal2011311180－5．4SangeethaTQuineSD．AntilipoperoxidativeandantioxidanteffectsofS-allylcysteinesulfoxideonisoproterenol-inducedmyo-cardialinfarctioninWistarratsJ．JBiochemMolToxicol2006204167－73．5SangeethaTDarlinQuineS．PreventiveeffectofS-allylcysteinesulfoxidealliinoncardiacmarkerenzymesandlipidsinisoprot-erenol-inducedmyocardialinjuryJ．JPharmPharmacol2006585617－23．6SangeethaTDarlinQuineS．PreventiveeffectofS-allylcysteinesulfoxidealliinonlysosomalhydrolasesandmembrane-boundATPasesinisoproterenol-inducedmyocardialinfarctioninWistarratsJ．JBiochemMolToxicol2007213118－24．7SangeethaTQuineSD．ProtectiveeffectofS-allylcysteinesulph-oxidealliinonglycoproteinsandhematologyinisoproterenolin-ducedmyocardialinfarctioninmaleWistarratsJ．JApplToxi-col2008285710－6．8SangeethaTQuineSD．PreventiveeffectofS-allylcysteinesulph-oxideAlliinonmitochondrialdysfunctioninnormalandisoprot-erenolinducedcardiotoxicityinmaleWistarratsahistopathologicalstudyJ．MolCellBiochem20093281－21－8．9．J．201335128－9．9ZhaoLGouPWangXetal．Effectsofgarlicactivitysubstancesonplasma-lipidsmetabolisminmicefedhighfatsdietJ．ChinTraditPatentMed201335128－9．10ElkayamAPelegEGrossmanEetal．Effectsofallicinoncardio-vascularriskfactorsinspontaneouslyhypertensiveratsJ．IsrMedAssocJ2013153170－3．11SantoshKMeenakshiSManishaT．Inhibitoryeffectofnoveldial-lyldisulfideanalogsonHMG-CoAreductaseexpressioninhyper-cholesterolemicratsCＲEBasapotentialupstreamtargetJ．Life·267·ChinesePharmacologicalBulletin2014Jun306Sci2009855－6211－9．12HuiCLikeWYanFetal．S-allyl-L-cysteinesulfoxideinhibitstumornecrosisfactor-alphainducedmonocyteadhesionandinter-cellularcelladhesionmolecule-1expressioninhumanumbilicalveinendothelialcellsJ．AnatＲecHoboken20102933421－30．13．、J．2011264328－30．13XuYYinZN．StudyonantioxidantactivityofalliinalliinaseandtheirmixtureinvitroJ．WestChinaJPharmSci2011264328－30．14GiovanniEManNJorgNSetal．Nrf2/AＲEregulatedantioxidantgeneexpressioninendothelialandsmoothmusclecellsinoxidativestressimplicationsforatherosclerosisandpreeclampsiaJ．ActaPhysiolSin2007592117－27．15LiXHLiCYXiangZGetal．Allicinamelioratescardiachy-pertrophyandfibrosisthroughenhancingofNrf2antioxidantsigna-lingpathwaysJ．CardiovascDrugsTher2012266457－65．16LouisXLMurphyＲThandapillySJetal．GarlicextractspreventoxidativestresshypertrophyandapoptosisincardiomyocytesarolefornitricoxideandhydrogensulfideJ．BMCComplementAlternMed201212140．17．+J．2003194478－80．17QinYDChenJWangHetal．Thestudyofmechanismonanti-tumoreffectofAlliin+AlliinaseJ．ChinPharmacolBull2003194478－80．18．SGC7901J．2012282213－7．18GuBYXiangSLZhuangYZetal．DiallyldisulfideinhibitmigrationandinvasioninhumangastriccancerSGC7901cellsanditsmechanismJ．ChinPharmacolBull2012