Amicon-Ultra-0.5超滤管

Amicon®Ultra-0.5CentrifugalFilterDevicesforvolumesupto500µLUserGuideForresearchuseonly;notforuseindiagnosticprocedures�����������������Fill�����������������Spin�����������������RecoverCollectPR02725_RevB.indd297/23/20093:01:49PMNoticeTheinformationinthisdocumentissubjecttochangewithoutnoticeandshouldnotbeconstruedasacommitmentbyMilliporeCorporationoranaffiliatedcorporation.NeitherMilliporeCorporationnoranyofitsaffiliatedcorporationsassumesresponsibilityforanyerrorsthatmayappearinthisdocument.Thismanualisbelievedtobecompleteandaccurateatthetimeofpublication.InnoeventshallMilliporeCorporationbeliableforincidentalorconsequentialdamagesinconnectionwithorarisingfromtheuseofthismanual.©2009MILLIPORECORPORATION.ALLRIGHTSRESERVED.PR02725Rev.B,07/09Millipore,Amicon,Centricon,Milli-Q,MultiScreen,andUltracelareregisteredtrademarksofMilliporeCorporation.TheMmarkisatrademarkofMilliporeCorporation.AlconoxandTerg-A-ZymeareregisteredtrademarksofAlconox,Inc.LubrolisaregisteredtrademarkofImperialChemicalIndustriesPLC.NonidetisatrademarkofRoyalDutch/ShellGroup.TritonisaregisteredtrademarkofUnionCarbideCorporation.TweenisaregisteredtrademarkofICIAmericas,Inc.PR02725_RevB.indd307/23/20093:01:49PMContentsIntroduction..........................................................................................................................................................1Applications.........................................................................................................................................................2MaterialsSupplied...............................................................................................................................................3RequiredEquipment............................................................................................................................................4Suitability.............................................................................................................................................................4DeviceStorage....................................................................................................................................................4Specifications......................................................................................................................................................5ChemicalCompatibility........................................................................................................................................7HowtoUseAmiconUltra-0.5CentrifugalFilterDevices......................................................................................9HowtoQuantitateRecoveries...........................................................................................................................11DirectWeighingProcedure........................................................................................................................11Performance-DNAConcentration....................................................................................................................14Performance-ProteinConcentration................................................................................................................15FlowRate...................................................................................................................................................15ProteinRetentionandConcentrateRecovery............................................................................................18MaximizingSampleRecovery...........................................................................................................................21DesaltingorDiafiltration.....................................................................................................................................22CentrifugalProductOrderingInformation..........................................................................................................23TechnicalAssistance.........................................................................................................................................24StandardWarranty.............................................................................................................................................25PR02725_RevB.indd317/23/20093:01:49PMIntroductionMillipore’sAmicon®Ultra-0.5centrifugalfilterdevicesprovidefastultrafiltration,withthecapabilityforhighconcentrationfactorsandeasyconcentraterecoveryfromdiluteandcomplexsamplematrices.Theverticaldesignandavailablemembranesurfaceareaprovidefastsampleprocessing,highsamplerecovery(typicallygreaterthan90%ofdilutestartingsolution),andthecapabilityfor30-foldconcentration.Typicalprocessingtimeis10to30minutesdependingonNominalMolecularWeightLimit(NMWL).Solutepolarizationandsubsequentfoulingofthemembraneareminimizedbytheverticaldesign,andaphysicaldeadstopinthefilterdevicepreventsspinningtodrynessandpotentialsampleloss.Efficientrecoveryoftheconcentratedsample(retain