3种硝化污泥性能的比较研究胡安辉

416四川大学学报(工程科学版)Vol.41No.6200911JOURNALOFSICHUANUNIVERSITY(ENGINEERINGSCIENCEEDITION)Nov.2009　　:1009-3087(2009)06-0089-083胡安辉,郑　平＊,胡宝兰(,310029)　:,3。,,,,,,,(63.58±4.19)%,;,,,,,(92.17±2.67)%,;(Rma/KSa),,。:;;;;;:X705:APerformanceofThreeKindsofNitrificationSludgeHUAn-hui,ZHENGPing＊,HUBao-lan(Dept.ofEnvironmentalEng.,ZhejiangUniv.,Hangzhou310029,China)Abstract:Batchexperimentswerecarriedouttostudytheperformancesofthreekindsofnitrificationsludgeofsee-dingsludge(S1)andnitrificationsludge(S2andS3)takenfromabioreactorrunwithinorganicororganicammo-nia-containingwastewaterforalongtime.Theperformancesofseedingsludge(S1)weresetascontrol,andtheni-trificationsludge(S2)showedahigherammoniaoxidationrate,alargerhalf-saturationconstant,astrongertoler-ancetonitrite,ahighernitriteoxidationrateandanaveragenitriteaccumulationof(63.58±4.19)%.Thepro-ducednitritecouldbefurtheroxidizedintonitrate.Thenitrificationsludge(S3)hadalowerammoniaoxidationrate,asmallerhalf-saturationconstant,aweakertolerancetonitrite,alowernitriteoxidationrateandanaveragenitriteaccumulationof(92.17±2.67)%.Theproducednitriteconcentrationcametostablebecauseitcouldnotbeenoxidizedintonitrate.Theratioofmaximumspecificammoniaoxidationratetohalf-saturationconstant(Rma/KSa)showedthattheactivityofnitritebacteriainbothnitrificationsludgeS2andS3wasincreased.Keywords:partialnitrification;nitrificationcapability;ammoniaoxidation;nitriteoxidation;nitriteaccumulation　　,。,:2008-11-02:(30770039);(2006AA06Z332);(Y507227):(1982-),,.:.　＊　E-mail:[e-mail],,“-”“-”[1-3]。,,,、[2]。,。DOI:10.15961/j.jsuese.2009.06.007、,,,-[4-7]。,,O2,[8],,[9]。,,。S1,,S2S3,,。,、,,()。,、,[10-11],3。1　1.1　S1:。A1-O1-A2-O2,O1。O15760m3,,,COD(160±57)mg·L-1,(99±16)mg·L-1,COD(129±52)mg·L-1,(77±18)mg·L-1,,(16.5±11)mg·L-1。S2:S1,。6.8L,,1.5a。(HRT)12h,840mg·L-1,(140±12)mg·L-1,(570±25)mg·L-1,(80±30)mg·L-1。S3:S1,。6.8L,,1.5。:COD(471±131)mg·L-1,(119±58)mg·L-1,C/N(3.57±1.50)。12h,:COD(248.9±127.6)mg·L-1,(69±57)mg·L-1,(30.5±13.4)mg·L-1,。1.2　,(g·L-1):KH2PO40.17,K2HPO43.27,0.04%3.5ml(pH),CaCl20.136,MgSO4·7H2O0.300,KH-CO30.500,(NH4)2SO4NaNO2,NaHCO3,pH7.0～8.2。:EDTA20.000g·L-1,FeSO45.000g·L-1,ZnSO4·7H2O0.430g·L-1,CoCl2·6H2O0.240g·L-1,MnCl2·4H2O0.990g·L-1,CuSO4·5H2O0.250g·L-1,NaMoO4·2H2O0.220g·L-1,NiCl2.6H2O0.190g·L-1,H3BO40.014g·L-1。1.3　S1、S2、S3,、,,。250ml,100ml。,(NH4)2SO4NaNO2。(28±0.5)℃,200r·min-1。,3,,。1.4　-;N-(1-);[12];pHpH(pHS-9V);[13]。2　2.1　32.1.1　氨氧化历程S1,1(a)。50～1000mg·L-1,,,90()41　,800mg·L-1,。1　S1/S2/S3Fig.1　TheNH4+-NprofilesforS1/S2/S3　　S2,1(b)。50～1000mg·L-1,,,,850mg·L-1,。,S2S1。S3,1(c)。50～1000mg·L-1,,400mg·L-1,4～10h,;,。。,S3S1,S2。3,S1,S2,S3。,,S2,;,,S3,。2.1.2　氨氧化动力学特征。,1。1　3Tab.1　AmmoniaoxidationratebythreetypesnitrificationsludgerespectivelyAmmoniaoxidationratebyS1/(mg(N)·g()-1·h-1)　R2/(g·L-1)/(mg·L-1)AmmoniaoxidationratebyS2/(mg(N)·g()-1·h-1)　R2/(g·L-1)/(mg·L-1)AmmoniaoxidationratebyS3/(mg(N)·g()-1·h-1)　R2/(g·L-1)/(mg·L-1)T14.8300.97691.434±0.14954.405T16.0530.98771.935±0.17850.2T14.3820.99912.894±0.09452.3T25.5640.98821.434±0.10499.98T26.4180.99392.044±0.07393.6T25.6810.99192.885±0.30698.85T35.5760.97971.451±0.115199.02T313.1430.9711.753±0.304216.9T36.4440.99902.711±0.309184.1T47.8710.99631.491±0.123347.82T415.4500.97311.837±0.083428.4T46.2680.99683.069±0.189425.7T511.9050.99591.511±0.116583.58T514.9510.99351.664±0.035605.3T56.5730.99883.095±0.526607.99T610.5310.98571.521±0.118757.95T620.1250.94121.669±0.168891.0T66.3820.98713.109±0.140810.65T79.7360.9861.521±0.130976.5T721.3770.93731.463±0.098990.0T77.2080.99433.006±0.129109391　6,:3　　1,2。S1、S2S3(Rma)11.95、24.16、6.97mg(N)·g()-1·h-1;(KSa)126.46、217.78、26.72mg(N)·L-1。S1,31∶2.02∶0.58;31∶1.72∶0.21;3(Rma/KSa)1∶1.18∶2.78。,,S2,,;,S3,,;Rma/KSa,(S2S3)。,;,,。2　Fig.2　FitcurveofammoniaoxidationusingMichealis-MentenmodelforS1/S2/S32.2　32.2.1　亚硝酸氧化动力学特征3,3。50～1000mg·L-1,,200mg·L-1,S1;400mg·L-1,S1。50mg·L-1100mg·L-1,S11.07mg(N)·g()-1·h-11.18mg(N)·g()-1·h-1。,S2,50mg·L-1100mg·L-1,S23.20mg(N)·g()-1·h-14.47mg(N)·g()-1·h-1。,S3,S3,,。3,3:S2S1S3。,S1S2,。200mg·L-1,S1,S2。3　3Fig.3　Thechangeofnitriteconcentrationwithtime2.2.2　亚硝酸抑制作用[14],92()41　[15-16],,pH。,,,pH,,。,34。4　Fig.4　Thechangeofammoniaconcentrationwithtimeinthepresenceofnitriteinhibition(cNH3,t-cNH3,0)/(t·c)(cNH3,t-cNH3,0),c,t)。0、200、800mg(N)·L-1,S113.31、10.55、11.09mg(N)·g()-1·h-1,,20.7%,;S214.49、14.52、14.42mg(N)·g()-1·h-1,;S36.05、3.78、1.05mg(N)·g()-1·h-1,,,37.5%82.6%。3,3:S2S1S3。,800mg(N)·L-1,。,,。2.3　32.3.1　不同氨氮浓度下的亚硝酸积累性能50～1000mg·L-1,,5(a)、(b)、(c)。50、100、200mg·L-1,S18、12、24h,18.5、67.14、142.9mg·L-1;S22、4、10h,44.5、69.7、140.8mg·L-1。S1、S2,。,S3,,,3.2.1S3。52.3mg·L-1,4h,S3,。()、(),3,5(d)、(e)、(d)。5(d)、(e)、(f),,S1(),50mg·L-1(100%),37%,800mg·L-1,91%,1000mg·L-1,56%。S2,60%～70%((63.58±4.19)%)。S3,93　6,:3(92.17±2.67)%。2.3.2　同一基质浓度下的亚硝酸积累性能600mg·L-1,,1.12、2.48、0mg·L-1。5　Fig.5　TheNO2--NprofileandtherangeofNO2--N/NtotandNO3--N/NtotundervariousNH4+-Nconcen-tration6　600mg(N)/LFig.6　TherangeofrelativeproductconcentrationwhenNH3=600mg(N)·L-1　　,S1-(dNO2-1/dNO3-1),(R2=0.8119),(7～8)∶1。S2-,dNO2-1/dNO3-1,,。S3-S1(R2=0.9622),(8～9)∶1。S1、S2、S3,90.8%、64.7%88.5%。S2,S