A2O工艺缺氧池中反硝化聚磷菌的比例特性研究及菌株鉴定周康群

:,,1963,,,。A2/O工艺缺氧池中反硝化聚磷菌的比例、特性研究及菌株鉴定周康群刘　晖孙彦富周遗品刘洁萍(,　510225)　　/、A2/O,(DPB),、。,DPB(PAO)21.5%。、DPB,PAO,。O2NO-3,O2;,COD30mg/LCOD180mg/L。DPBCOD。,A2/O,DOCOD。　　A2/O　　　DenitrifyingphosphateaccumulatingbacteriaintheanoxicreactorofanA2/Owastewatertreatmentsystem　ZhouKan-gqun,LiuHui,SunYanfu,ZhouYipin,LiuJieping.(DepartmentofEnvironmentScienceandEngineering,ZhongkaiAgrotechnicalCollege,GuangzhouGuangdong510225)Abstract:　Manydenitrifyingphosphateaccumulatingbacteria(DPB)werefoundintheanoxicbioreactormixedliquorofanA2/OwastewatertreatmentsysteminsouthernChina.TheDPBsaccountedforabout21.5%oftotalphosphateaccumulatingorganisms(PAO)present.Enterobacteriaceaesp.,Aeromonassp.andPseudomonassp.wereDPBs;Acinetobactersp.wasaPAO;Staphylococcussp.andMicrococcussp.werestrictlydenitrifyingbacteri-a.Thenatureofelectronicacceptorand/ortheamountoforganicmatterspresentinthereactoraffectedthedenitrifi-cationandphosphorusaccumulationcharacteristicsofDPB;Enterobacteriaceaesp.consumedoxygenfirstwhenoxy-genandnitratewerebothpresent,andunderanoxicconditions,accumulatedmorephosphorusatalowerCOD(30vs.180mg/L).PropercontrolofdissolvedoxygenandCODoftheanoxicmixedliquorwasnecessaryforimprovingperformanceofaconventionalA2/Otreatmentsystem.Keywords:　A2/OAnoxictankDenitrifyingphosphorusaccumulationDenitrifyingphosphorusbacteria　　A2/O,(PAO),,,NO-3[1]。KUBA[2]777UCT。,PAO,O2NO-3,(DPB)[3]。KUBA[2]778PAODPB,NO-3DPBPAO。,,。,[4,5]。　　,A2/ODPBPAO,DPB,。A2/O,DPB,,A2/O,。1　1.1试验设备　　/,/(N2/)/(),·437·　A2/O、DOI:10.15985/j.cnki.1001-3865.2007.06.0101、2,1.5L。1　/Fig.1　Flowdiagramfortheanaerobic/anoxicbioreactor1—N2;2—;3—;4—;5—2　/Fig.2　Flowdiagramfortheanaerobic/aerobicbioreactor1—;2—;3—;4—;5—1.2供试材料1.2.1　　　A2/O,。1.2.2　　　(1)　　[6]133:20g,Na2HPO42g,1g,KNO31g,1g,pH=7.0～7.2,1000mL。　　PAOⅠ[7]:NaAc3.68g,Na2HPO428.73mg,NH4Cl57.27mg,MgSO4131.82mg,K2SO426.74mg,CaCl217.2mg,HEPESbuffer12g,1g,pH=7.0,2mL,1000mL。　　PAOⅡ:NaAc1.20g,Na2HPO428.73mg,NH4Cl57.27mg,MgSO4131.82mg,K2SO426.74mg,CaCl217.2mg,HEPESbuffer12g,1g,pH=7.0,2mL,1000mL。　　:EDTA50g,FeSO45g,CuSO41.6g,MnCl25g;(NH4)6Mo7O240.1g;H3BO350mg;KI10mg,MoCl250mg,1000mL。　　(2)、　　:3g,10g,NaCl5g,1000mL,18g,pH=7.0～7.2。1.2.3　　　1。1　Table1　Compositionoftheartificalwastewater/(mg·L-1)COD(NaAc)100～300NH4Cl14K2HPO44～9MgSO44.5ZnCl20.1FeCl30.5MnSO40.1KI0.11.3实验方法1.3.1　DPB　　,2500mg/L,/,30min((ORP)-120～-100mV),,25mg/LNO-3-N,,/。/,O2。,3000r/min1min,COD、PO3-4-PNO-3-N,ORP,HClNaOHpH7±0.2[8]。1.3.2　、PAO、　　MPN,、,,PAOⅠ,27～30℃7～8d。(、),MPN。,35℃48h,3～4,,。1.3.3　　　、PAO、、、、、、、(18～24h)、、,[9],DBP。1.3.4　　　(1)　　、:1.3.2·438·　　29　6　20076PAOⅠ,27～30℃6～7d(108～1010/mL)。　　COD:1.3.2PAOⅡ,27～30℃6～7d(108～1010/mL),COD30mg/L。　　(2)、　　1300mL、,//,N2ORP-100～-80mV,7mg/LPO3-4-P,28mg/LNO-3-N,NO-3-N/PO3-4-P4/1,NO-3,ORP0～100mV;O2,ORP100～200mV。,3000r/min1min,NO-3-NPO3-4-P。ORP,HClNaOHpH7±0.2。　　(3)COD　　1300mLCOD,/,N2ORP-100～-80mV,PO3-4-P(7mg/L)NO-3-N(28mg/L),NO-3-N/PO3-4-P4/1[6]134。NaAcCOD180mg/L(COD30mg/L),NaAc,COD30mg/L,NO-3,,3000r/min1min,COD、PO3-4-PNO-3-N,ORP,HClNaOHpH7±0.2。1.3.5　　　ORPSUNTEXTS-2ORP;NO-3-N;PO3-4-P;COD5B-1A。2　2.1缺氧池中的DPB　　1.3.13。30minPO3-4-P5.72mg/L8.92mg/L,(MPO3-4-P/(MMLSS·h))2.56g/(kg·h),COD300mg/L105mg/L,COD65%。,PAO,,PAO,PAOβ-(PHB),。、,,60min,PO3-4-P8.92mg/L2.83mg/L,PO3-4-P68.3%,(MPO3-4-P/(MMLSS·h))2.44g/(kg·h)。,60min,PO3-4-P8.92mg/L7.61mg/L,1.31mg/L,(MPO3-4-P/(MMLSS·h))0.524g/(kg·h),PO3-4-P14.7%;NO-3-N25.0mg/L1.7mg/L,NO-3-N93.2%,60min(MNO-3-N/(MMLSS·h))9.32g/(kg·h)。,60minCOD105mg/L9mg/L。;CODNO-3,[10]45-49,。3　A2/O、Fig.3　ConcentrationprofilesforCOD,phosphateandnitrateshowingphosphatereleasefromtheanoxicactivatedsludgeanditssubsequentadsorptionunderaerobicandanoxicconditions　　,60min·439·　A2/O、2　A2/O1)Table2　CharacteristicsofpureculturebacteriaisolatedfromtheanoxicbioreactorusingthedenitrifyingmediaF1F2F3F4F5、、、、、、、、、、、、、、、、、、、、、×0.1μm×1.3μm×0.6μm×2.5μm0.4～0.8μm0.9～1.5μm0.8～2.0μm---+++-+--+++++-----+++++-++-+f-fffND+++++++----NDNDND6.5%()NaClND+ND++--NDNDNDNDND+NDNDNDND-NDND　　:1)+,≥90%;-,≥90%;ND,;f,。3。2.44g/(kg·h),0.524g/(kg·h),KUBA[11],DPBPAO。DPBPAO21.5%,KUBA[2]785DPBDBPPAO50%。DPB。2.2筛选培养过程细菌总数变化　　PAOⅠ,23。　　2、3,A2/O、、、;A2/OPAOⅠ、。,()[6]134,、,。3　A2/OPAOⅠTable3　CharacteristicsofpureculturebacteriaisolatedfromtheanoxicbioreactorusingthephosphorusaccumulationmediaⅠJ1J2J3、、、、、、、、、、　　、、×0.1μm×1.3μm×0.9μm×2.5μm0.4～0.8μm---+-++-+---+-+--+f-fND+++++-NDND6.5%NaClNDNDND-NDNDNDND+NDND-·440·　　29　6　20076、。、,、。　　,、、,DPB。,、、,DPB,[12]。,PAOⅠ27.5%20.3%,[6]135,,2.3。　　PAOⅠ,、、,PAO。,,NO-3-NN2。　　,A2/O、、、、,、,DPB。DPBPAO21.5%,DPBA2/O,DPBPAO,,DPB,。A2/O,、DPB。2.3肠杆菌科释磷/聚磷试验2.3.1　、　　1.3.44。180min,PO3-4-P7.01mg/L3.22mg/L,54.1%,PO3-4-P7.01mg/L6.20mg/L,11.4%。,2.1,O2,NO-3DPB;O2NO-3,O2。,O2,。4　O2/NO-3Fig.4　Effectofoxygen/nitrateastheelectronicacceptoronphosphateaccumulationofEnterbacteriaceaesp.2.3.2　COD　　5,120min,COD30mg/LPO3-4-P7.02mg/L4.04mg/L,NO-3-N28.00mg/L13.96mg/L,2.98mg/L,PO-4-P42.2%,NO-3-N50.1%;COD180mg/LPO3-4-P7.02mg/L5.69mg/L,1.33mg/L,18.7%,NO-3-N28.00mg/L9.66mg/L,65.5%。COD,COD,NO-3。CODNO-3,。COD,,CODDPBNO-3[10]46。5　CODFig.5　EffectofCODconcentrationondenitrifyingphosphateaccumulationofEnterbacteriacea