Anammox反应器中氨氧化菌的分离鉴定及特性研究胡宝兰

浙江大学学报()　27(3):314～316,2001JournalofZhejiangUniversity(Agric.&LifeSci.)　　:2000-08-21:(30070017)(596043):(1972-),,,,.:1008-9209(2001)03-0314-03Anammox胡宝兰1,郑平1,管丽莉2(1.,310029;2.,310024)　:从Anammox反应器污泥中分离了6株好氧氨氧化菌,并对其中的N1菌株进行了生物学特性研究,试验证实该菌株具有厌氧氨氧化能力.　　:厌氧氨氧化;氨氧化菌;分离;鉴定;特性:Q939.11+1　　　:AHUBao-lan1,ZHENGPing1,GUANLi-li(1.Dept.ofEnvironmentalEngineering,ZhejiangUniv.,Hangzhou310029,China;2.HangzhouEnvironmentalProtectionBureau,Hangzhou310024,China)Isolation,identificationandcharacteristicsofammonia-oxidationbacteriafromAnammoxreactor.JournalofZhejiangUniversity(Agric.&LifeSci.),2001,27(3):314～316Abstract:6strainsofammonia-oxidationbacteriawereisolatedfromanAnammoxreactor,andthebio-logicalcharacteristicsofstrainN1werestudied.TheresultsshowedthatstrainN1hadtheabilitytooxidizeammoniumanaerobically.Keywords:anaerobicammoniaoxidation;ammonia-oxidationbacteria;isolation;identification;charac-teristics　　、(Anammox)[1,2].,.　　Anammox,,[3].1　材料与方法1.1　　　Anammox,,NH4+—N28mg/L、.1.2　1.2.1　细胞形态　　[4～7].1.2.2　细胞结构　　[7].1.2.3　细菌G+C%含量　　.(Tm).E.coli.365(G+C%50.4%)[8].1.2.4　氨氧化菌的最适pH　　(3000r/min,10min),.pH6.0,6.5,7.0,7.5,8.0,8.5,9.0.30℃(150r/min)...1.3　　　(3000r/min,10min)N1.10mL70mL110mL,1mol/L(NH4)2SO41mol/LNaNO2N28mg/L.,,95%Ar5%CO2(20min),.,.30℃,3.1mL,HN4+—NNO2-—N.3.2　结果与讨论2.1　　　,6,N1、N2、N3、N4、N5、N6.,N1.N1.2.2　N12.2.1　N1菌株的形态　　N1,,.,.,,.1　N1(×20000)Fig.1　AppearanceofstrainN1　　Olympus,;,,;,0.8～1.2μm×1.0～1.5μm;.1.2.2.2　N1菌株的内膜结构　　N1,(2).2　N1(×54000)Fig.2　InnermenbraneofstrainN12.2.3　N1菌株G+C%含量　　N1G+C%67.8%.2.2.4　N1菌株的最适pH　　pHN13.pH6.0pH7.5,;pH7.5pH8.5,;pH8.5,,.N1pH8.5.Painter[9].3　pHN1Fig.3　EffectofpHonammoniaoxidationactivityofstrainN12.3　　　N1,.N1CK1,,;CK2,;N1,(4).,315　3　,　　Anammox　　　　　.,(5).15d,(NH4+—NNO2—N)N1(5).4　N1Fig.4　AnaerobicammoniaconversionbystrainN15　Fig.5　Disappearanceofammoniaandnitriteunderanaerobiccondition6　40dN1(×40000)Fig.6　InteriorstructureofstrainN1after40-dayanaerobicculture　　,NH4+—NNO2-—N.,().40d,,,.(6).:,N1,.3　结　论　　Anammox.,.　　、、,N1Nitrosomonas,G+C%..References:[1]　MulderA,AAvandeGraaf,LARobertson,etal.Anaerobicammoniumoxidationdiscoveredinadenitrify-ingfluidizedbedreactor[J].FEMSMicrobEcol,1995,16:177-183.[2]　AAvandeGraaf,LARobertson,MSMJetten,etal,Autotrophicgrowthofanaerobicammonium-oxidizingmicroorganismsinafluidizedbedreactor[J].Microbiolo-gy,1996,142:2187-2196.[3]　AAvandeGraaf,MulderA,PDebruijn,etal,Anaer-obicoxidationofammoniumisabiologicallymediatedprocess[J].ApplEnvironMicrobiol,1996,61:1246-1251.[4]　StaleyRL,BryantMP,PfennigN,etal.Bergy'smanualofsystematicbacteriology(vol3)[M].Balti-more:WilliamsP&Hensyl,1989.1818-1833.[5]　HoltJG,KriegNR,SneathPHA,etal.Bergey′smanualofdeterminativebacteriology(9thedition)[M].Baltimore:Williams&Wilkins,1994:427-455.[6]　ZURuo-fu,HUBao-long(,),TeachingMaterialofMicrobiologyExperiment()[M].Shanghai:FudanUniversityPress,1993.(inChinese)[7]　LINJun-an,GAOJin-liang,HONGJan(,,),AppliedBiologyElectronMicroscopicTech-nique()[M].Shenyang:LiaoningScienceandtechnologyPress,1989.(inChinese)[8]　LINWan-min,GUOZhao-biao,GAOShu-de,etal(,,,).DeterminetheGCContentofBacterialDNAbyHighTemperatureDenatureMethod[J].JournalofMicrobiology(),1981,8(5):245-247.(inChinese)[9]　PainterHA,JELoveless.EffectoftemperatureandpHvalueonthegrowth-rateconstantsofnitrifyingbacteriaintheactivatedsludgeprocess[J].WatRes,1983,17:238-246.316　　　　　浙江大学学报()　27