ANAMMOX与反硝化协同脱氮反省略启动及有机负荷对其运行性能的影响孙艳波

　60　10　　　　　　　　　　　　Vol.60　No.10　200910　　CIESC　Journal　　　October　2009ANAMMOX孙艳波,周少奇,李伙生,覃芳慧(,510006):UASB-ANAMMOXTN0.26kg·m-3·d-1,(),TN1,35dANAMMOX,、、TNCOD95.3%、99.1%、94.0%93.2%,∶∶1∶1.34∶0.03。ANAM-MOX。,;,,,。。:;;;;:X703　　　　　　:A:0438-1157(2009)10-2596-07Start-upofANAMMOX-denitrificationreactorandeffectoforganicloadingonitsperformanceofsynergisticinteractionSUNYanbo,ZHOUShaoqi,LIHuosheng,QINFanghui(SchoolofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou510006,Guangdong,China)Abstract:Continuousadditionoforganicmatter(glucose),atanorganicloadingtototalnitrogen(TN)ratioof1,wasconductedaftertheUASB-ANAMMOXreactorhadreachedthestableoperationphase.TheANAMMOX-denitrificationreactorwassuccessfullystartedwithin35dundertheconditionofinfluentnitrogenloadingstablyat0.26kg·m-3·d-1.Theremovalefficienciesofammonia,nitrite,TNandCODwereupto95.3%,99.1%,94.0%and93.2%,respectively.Theaverageratioofremovedammonia,removednitriteandgeneratednitratewas1∶1.34∶0.03atthestablephase.TheeffectoforganicloadingshockontheANAMMOX-denitrificationreactorwasalsoinvestigated.Theresultsdemonstratedthatthesharpdecreaseinorganicloadinghadlittleeffectonthereactor,whilethesharpincreaseinorganicloadingposednotableinfluenceonthereactor.Stablenitrogenremovalwasachievedafteracclimatization,althoughtheeffluentqualitydeteriorated.Theeffluentqualitycouldbeimprovedbysubstantiallyreducingtheinfluentorganicloading.TheANAMMOX-denitrificationreactorhadstrongorganicloading-resistance.Keywords:anaerobicammoniumoxidation;denitrification;synergisticinteraction;organicloading;performance　　2009-04-20,2009-05-07。:。:(1984—),,。:(20377013);(2006B36703002);(020959)。　　　Receiveddate:2009-04-20.Correspondingauthor:Prof.ZHOUShaoqi,[e-mail]:supportedbytheNationalNaturalScienceFoundationofChina(20377013),theScienceandTechnologyKeyProjectofGuangdongProvince(2006B36703002)andtheNaturalScienceFoundationofGuangdongProvince(020959).　　(ANAMMOX),NH+4,NO-2NO-3,NH+4、NO-2NO-3N2[1]。ANAMMOX、[2],[3-13]。ANAMMOX,,[3],ANAMMOX[4],,ANAMMOX,。[4-9],ANAMMOX,,。ANAMMOX[7-9],ANAMMOX。ANAMMOX,ANAMMOX,,。1　1.1　1。,3.2L,,UASB(32±1)℃。。1.2　,(NH4)2SO4NaNO2,。NaHCO3、KH2PO4。Ⅰ(g·L-1):EDTA5.000,FeSO45.000;Ⅱ(g·L-1):ZnSO4·7H2O0.430,CuSO4·5H2O0.250,MnCl2·4H2O0.990,NiCl2·6H2O0.190,CoCl2·6H2O0.24,H3BO40.014。Ⅰ、Ⅱ1.0ml·L-1。pH7.6～8.2。1　UASBFig.1　UASBreactoranditsflowdiagram1—circlethermostaticwaterbath;2—thermometer;3—exhaust;4—bufferbottle;5—influentpump;6—gas-solid-liquidseparator;7—influent;8—heatpreservation;9—sampling;10—UASBreactor;11—effluent　1.3　《》[14]:COD,;,;,N-(1-)-;,;pHpHS-9V。1.4　(HRT)24h,TN0.26kgTN·m-3·d-1,1∶1.32。,0.26kg·m-3·d-1,ANAMMOX;,。0.13、0.39、0.26、0.13kg·m-3·d-1,TN1/2、3/2、11/2。1。1　Table1　CharacteristicsofinfluentParameterTime/dCOD/mg·L-1TN/mg·L-1NH+4-N/mg·L-1NO-2-N/mg·L-1start-upphase1—55260260112148organicloadingshockphase56—7013026011214871—111390260112148112—133260260112148134—180130260112148·2597·　10　　:ANAMMOX2　2.1　ANAMMOX,ANAMMOX。、TN98%、99%85%,NH+4-N∶NO-2-N∶NO-3-N1∶1.30∶0.26,Strous[3]。,。2.2　[7],CO2,NO-3-N,;,CO2,ANAMMOX,。ANAMMOX,ANAMMOX。CODTN2,3,4。2,COD3:(1～2d)、(3～35d)(36～55d)。:COD89%,CODNO-3-N,COD,,。:,(3～23d)COD,45%～55%,,;,,COD,35d89%;,。:COD93%,COD13～22mg·L-1。3,ANAMMOX,。2d,,96%99%。(3～10d),,3.53mg·L-136.1mg·L-1,68.1%,ANAMMOX[4];,,。2,7～23dCOD50%,ANAMMOX,81.4%。,,23d20.8mg·L-135d·2598·　　　　　60　12.3mg·L-1,89.8%,98%。,35dANAMMOX。35d,、COD,、95.3%99.1%,TNCOD15.5mg·L-116.2mg·L-1,94.0%93.2%。4,1～2d,28.2mg·L-1,1∶1.33∶0.26,,,。(3～16d),(α),1.85;(β)0.20,17.6mg·L-1;α,β,。17～35d,5.6mg·L-1,β0.06,;α1.52,ANAM-MOX,。35d,β3.2mg·L-10.03,αANAMMOX1.34,1.32。ANAMMOX。,ANAMMOX。34,,,ANAMMOX,95.3%,ANAMMOX。[10]ANAM-MOXα0.750.95;[11]ANA-MMOXα1.0;Dosta[15]α1.05。ANAMMOX,ANAMMOX“”ANAM-MOX,。[7]C/N(η)YCη1=COD/NO-3-N=2.857/(1-1.628YC)(1)η2=COD/NO-2-N=1.714/(1-1.628YC)(2)1,TN,COD0.26η1。YC≈0.15[2],η1≈3.8,η2≈2.3,,COD1.0。η1、η2,COD:COD99mg·L-1,COD142mg·L-1,0.7。,ANAMMOX61.7mg·L-181.4mg·L-1,43.1%56.9%,ANAMMOX∶1∶0.77,1∶1.30,ANAMMOX。ANAMMOX:(1)“”;(2)。ANAMMOX。,UASB-ANAMMOX()(35d)ANA-MMOX,11.340.03,ANAMMOX,,。2.3　　　TN0.26kg·m-3·d-1,11.32。56d,·2599·　10　　:ANAMMOX,ANAMMOX。TN1/2、3/2、11/2。5,6。56～70d,0.26kg·m-3·d-10.13kg·m-3·d-1,。56,,,COD8～15mg·L-1,TN15mg·L-1。。71d0.13kg·m-3·d-10.39kg·m-3·d-1,。3d,COD12.8mg·L-1100.3mg·L-1,74.1%,;,81dCOD92%,,。6,,99%;,3.1mg·L-1;,77d22.1mg·L-1,,85d62.8mg·L-1,41.6%,TN71.0%,ANAMMOX;,ANAMMOX,。85d,,102dTN53%75%,ANAMMOX,,;TN53.2mg·L-167.1mg·L-1,。,ANAM-MOX,,,。COD3.5,,3.0。,,112d0.26kg·m-3·d-1,TN3/21。5,。6,,TN,,15d42mg·L-156mg·L-1,TN79%,4%。0.26kg·m-3·d-1,,。,134dTN1/2,·2600·　　　　　60　。6,0.13kg·m-3·d-1,,,2d,144d25.1mg·L-112.7mg·L-1,68.5%,TN68%;,ANAMMOX,18d,152d,,178d79%,ANAMMOX,164d96%,99%,2.8mg·L-1,CODTN92.1%85.9%,CODTN11.5mg·L-136.5mg·L-1,11.650.03,。,。,ANAMMOX;。80%,ANAMMOX,ANAMMOX[3],。3　　(1)TN0.26kg·m-3·d-1,11.32,(),TN1,35dANAMMOX,11.340.03,ANAM-MOX10.77。、、TNCOD95.3%、99.1%、94.0%93.2%,ANAMMOX。。(2)ANAMMOX;(),,;,。。(3),COD1.0～3.0。AN-AMMOX“”ANMMOX。References[1]　MulderA,vandeGraafAA,RobertsonLA,etal.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidizedbedreactor.FEMSMicrobiol.Ecol.,1995,16:17