实验三-醋酸纤维素薄膜电泳法分离血清蛋白

:(1954),,,,.ATP(,130021):,,(ATP),ATP,ATP(pH),,ATP,ATP.:;(ATP);ATP:X832:A:100921288(2007)0320007205,.,,,.(MLSS)(MLVSS)(SV)(SVI)(TS),F/M.F(Food);M(Microorganisms),,MLSS(MLVSS).,,MLSS(MLVSS),MLSS(MLVSS),(),,.,(DHA)(SOUR)(ATP),,ATP,,,,ATP,,.ATP,[1-2].1ATP(AdenosineTriphosphate,ATP),C10H16O13N5P3,:PHOOOHOPOOHOPOOHOCH2CHCOHCOHOCHNCHNCCCNH2NCHN,53,,2,1.301565kJ/mol24320079JournalofJilinArchitecturalandCivilEngineeringInstituteVol.24No.3Sep12007(713kcal/mol),ATP(ADP).ATP-617(22,31095%),ATPADP(AMP)pKa1[3].1pKapHmax/nmEmax/10-3Emax/10-3250/260280/260290/260AMP347122110210257151114120184012201038AMP347122317AMP347122612614712259151415100179011501009ADP42712011022571510141501850121ADP427120319ADP4271206116177122591514151401780116ATP5071201102257141714130185012201027ATP507120410ATP507120615712259151415140180011401008ATPADP,AMP,ATP,.,ATP260nm.ATPADPAMP(Pi),ATPADPAMPpH.,ANADH,.Mcelroy,:Mg2+,ATP,,AMP,(PPi).,,CO2H2O.,L-AMP,LAMP,ATP,ATP[4-6],:LH2+ATP+EZE-LH2-AMP+PPiE-LH2-AMP+O2vOL+AMP+CO2+h,LH2();E;E-LH2-AMP-;PPi;AMP;OL;h.2ATPATP510-16g510-15g,,pH,(CN-,S2-).211pHATP,,.,pH,,,.pH68,,ATP.212ATPRoeP1C[7]S2-(010mg/L)CN-(0110mg/L),,.2.2CN-,S2-ATPS2-(mg/L)0246810ATP(RLU/mgMLSS)481004050010313001023050101415010992010CN-(mg/L)0012014016018110ATP(RLU/mgMLSS)4810036200102803010210001011300104350102,S2-CN-ATP,ATP824=512;CN-1m=0150(1m:.ATP100%,,ATP,50%,,,50%1m).1SVI-ATP213ATP,MLSS,,,MLSS,,.,,ATP114g/mgVSS212g/mgVSS,SVI.SVI250mL/g,1[8].3ATP,ATP.[9]ATP467,ATP,,,,ATP,3.3ATP/d1571113810310810310(IA),,:IA=(/)100,,.IA,3:(1)IA150,;(2)150IA50,;(3)IA50,.[10]ATP,ATP,,,,ATP.,COD250mg/L2000mg/LATP.,COD1000mg/L,ATP5RLU/mgMLSS;COD1000mg/L,COD,,ATP5RLU/mgMLSS,1RLU/mgMLSS.,,COD1000mg/L,COD150mg/L,,.JudyA.[11],,,DO015mg/L,ATP3RLU/mgMLSS,,;DO015mg/L2mg/L,;DO2mg/L6mg/L,.,,DO2mg/L,DO.TobinR.S.[12]1535ATP,2.2,,,,2515115,3525,,2593,,:ATP[13]ATP(OUR)(FDA),ATP.ATP,ATP.,ATP.ATP,,,COD,.4ATPATP,,,.(1)ATP,.L,ELAMP.L,,ELAMP,.,,.(2)ATP.103/mL.103/mL,103/mL.(3)ATP,ATP.,ATP,ATP.(4)ATP,ATP,.,.,,,.,,,,.,pHATP,,ATP,,.5ATP(5min,),,.ATPCOD,,,,,.[1],1J1,1993(2):58-6210124[2]()1ATPJ1,1992(3):13-181[3],,1M1:,19941[4],,,,1ATPJ1,2003,17(2):30-331[5],,1ATPJ1,1998,4(4):2412441[6]JamesW.Patterson,PatrickL.Brezonik,andHughD.Putnam.MeasurementandSignificanceofAdenosineTriphosphateinActivatedSludgeJ.EnvironmentaiScienceTechnology,1970,4(7):569-5751[7]RoeP.C,BhagatS.K.AdenosinetriphosphareasacontrolparametertoactivatedsludgeprocessesJ.J.Wat.Pollut.ControlFed.,1999,54:244-254.[8],1M1:,2002:117-1191[9],,1ATPJ1,1996(1):1-41[10]1J1,2002(6):56-581[11]JudyA,etal.ATP,oxygenuptakerateandint-dehydrogenaseaetivityofactionmycetefoamsJ.Wat.Res.,1985(7):917-921.[12]TobinR.S,etal.AnimprovedmethodforthedeterminationofATPinenvironmentalsamplesJ.Wat.Res.,1978(12):783-92.[13]JorgensenP.E.,EriksenT.,JensenB.K.,EstimationofviablebiomassinwastewaterandactivatedsludgebydeterminationofATP.Oxy2genutilizationrateandFDAhydrolysisJ.Wat.Res.,1992(11):1495-1501.StatusofApplicationandStudyofATPMeasurementinActivatedSludgeProcessYINJun,SANGLei,LILin(SchoolofMunicipalandEnvironmentalEngineering,JilinArchitecturalandCivilEngineeringInstitute,Changchun130021)Abstract:Traditionalindexeswhichareusedtomeasuresludgeactivityarentexactincontrolofactivatedsludgeprocess,soadenosinetriphosphate(ATP)becomesthenewindexwhichreflectsmicrobialviabilityofactivatedsludge.Inthispaper,partphysicalandchemicalqualityofATP,andprincipleandmethodsofmeasurementtech2nologyareintroduced,relationshipsofATPdensityandenvironmentelementssuchaspH,toxicantor,activatedsludgedensityarestudied.TheachievementsofATPmeasurementappliedinactivatedsludgeprocessispositive.AffectelementsofstudyandapplicationofATPmeasurementistentativelyanalyzed.Keywords:activatedsludgeprocess;adenosinetriphosphate;ATPmeasurement113,,:ATP