Ca2在好氧颗粒污泥形成中的作用杨新萍

31520105ENVIRONMENTALSCIENCEVol.31No.5May2010Ca2+*210095Ca2+0～200mg/LCa2+.SBRⅠ、ⅡCa2+30、100mg/L20d.50dSBRⅠMLSSSVISBRⅡ80dSBRⅠ、ⅡMLSS7.5g/LSVI46～48mL/g、45～47mL/g.90dSBRⅠ、ⅡZetaζ－12.5～－12.6mVCa2+0～200mg/L60dZeta－14.1～－14.4mVCa2+ZetaZeta.Ca2+Ca2+.SBRⅠ、ⅡCa、Mg、K、NaFe4.42%、7.82%.EPS.SBRⅡSBRⅠ70dSBRⅠ、ⅡNH+4、COD90%TN、TP65%～70%.Ca2+ZetaX522A0250-3301201005-1269-052009-07-142009-09-288632006AA06Z3141972～[e-mail]*[e-mail]2+intheFormationofGlucose-fedAerobicGranularSludgeinSequencingBatchReactorYANGXin-pingHANJiaoZHOULi-xiangDepartmentofEnvironmentalEngineeringCollegeofResourcesandEnvironmentalSciencesNanjingAgriculturalUniversityNanjing210095ChinaAbstractInfluenceofCa2+onglucose-fedaerobicgranulewasinvestigatedthroughsequencingbatchreactorSBRandshakingflaskexperiments.GranulesoftwoSBRsspikedwith30mg/Land100mg/LofCa2+couldbeobservedvisuallyonday20.Inthefirst50daystheSBRⅡhadhighermixedliquorsuspendedsolidMLSSandlowersludgevolumeindexSVIthanthoseofSBRⅠ.After50daysthedifferenceofgranuleperformanceintworeactorsgraduallybereducedandlittledifferenceswerefoundonday80ormoreforexampleMLSSofSBRⅠⅡwas7.5g/LandSVIarrivedat46-48mL/gand45-47mL/grespectively.After90dayssludgeZetapotentialofSBRⅠⅡreached－12.5-－12.6mV.Furthermorewithinfluentof0-200mg/LCa2+sludgeZetapotentialofshakingflaskreached－14.4-14after50days.Ca2+concentrationofinfluenthadlittleinfluenceonZetapotentialofsludgeduringgranulationprocess.MicrobiologicalobservationsconfirmedsomeappreciablechangesinmicroorganismpopulationanddiversitieswiththeincreaseofCa2+concentrationofinfluent.TheroleofCa2+inbioflocculationofgranulecanbemainlyattributedtoitscationbridginginsteadofchargeneutralization.TheelementalanalysisofseedsludgeandaerobicgranulebyX-rayfluorescenceXRFshowedthatthecontentofCaMgKandNaingranulewerelessthanseedsludgeexpectforFeincreasing4.42%and7.82%respectively.DivalentmetalionssuchasCa2+andFe2+wereprobablyconstituentofbiopolymer.ItwaspossiblethatthebindingofdivalentmetalionwithextracellularpolymericsubstancesEPSenhancedthegranulationofseedsludge.ConsequentlythepollutantremovalefficiencyofSBRⅡwashigherthanthatofSBRⅠthroughtoutthetrial.BothNH+4-NandCODremovalefficienciesreached90%andbothtotalNandtotalPremovalefficiencywere65%-70%afterday70.KeywordsaerobicgranularsludgeCa2+ZetapotentialsequencingbatchreactorSBRremovalefficiency、、12.、1～3.2、345.DOI:10.13227/j.hjkx.2010.05.01831Ca2+6～11、、12～15..SBRZetaCa2+SBRCa2+SBR.11.1A2/O.SBR4L/3.22g/L.mg/L1500、NH4Cl200、K2HPO4·3H2O45、MgSO4·7H2O25、FeSO4·7H2O20CaCl2·2H2O1mL/Lmg/LH3BO3150、CoCl2·6H2O150、CuSO4·5H2O30、FeCl3·6H2O150、KI30、MnCl2·2H2O120、Na2Mo7O24·2H2O60、ZnSO4·7H2O120.NaHCO3pH6.5～7.5.1.22SBR1、1.21.SBR300mm×240mm×550mm××21.6L.、.0.25m3/h25±1℃25℃.SBR16h3min、325min、3min、4min、25min4/d.500mL250mLSBR25℃、180r/minSBR2.DOSBR.1.3Ca2+2SBRCa2+.SBRⅠCa2+30mg/LSBRⅡCa2+100mg/L.、20%50%.Ca2+mg/L0、15、30、50、100、150、2001.150%.1.4NikonEclipse80iSBRXPW4030/45BFinlandZetaColloidalDynamicsIncUSADOThermoOrion3starpHpHS-3CpHMLSS、SV、SVI16CODCODThermoAQ4001TNGB11894-1989NH+4GB7479-1987TPGB11893-1989.22.1SBRSVI、MLSS、DO12dSBRⅠ、ⅡSVISBRⅠSVI299mL/gSBRⅡSVI209mL/g1.SBRⅠ、Ⅱ40d、28dSVI60mL/g70d46～50mL/g、46～47mL/g.SBRⅡSBRⅠ.MLSS3.22g/L10dMLSS20%50%MLSS1.Ca2+100mg/LSBRⅡMLSSCa2+30mg/LSBRⅠSBRⅠ80dMLSS7.5～7.6g/LSBRⅡ60dMLSS7.6～7.7g/L.SBRⅡSBRⅠ80d.07215Ca2+1SVI、MLSSFig.1SVIMLSSforSBRduringoperationperiodSBRDOCa2+DO.2SBRⅠ、ⅡDO.2DOFig.2DOforSBRduringaerationoperationperiod2.2SBRZeta2、3479～11、3814151718.SBR、Zeta3、4.Zeta－16.8mV30dCa2+100mg/LSBRⅡZeta30dSBRⅠ、ⅡZeta－12.6mVZeta3.9mV.460dCa2+3SBRZetaFig.3ZetapotentialforSBRduringoperationperiod4ZetaFig.4ZetapotentialforflaskduringoperationperiodZeta42dCa2+ZetaZeta－15.5～－15.3mV60d－14.1～－14.3mV.45d、.Ca2+Zeta.19FeⅢZeta20Ca2+、Mg2+、K+、Na+ZetaPavoni21.2.3SBR172131.SBR.4dSBRⅡSBRⅠ..20d、1.0～3.5mm..Ca2+100mg/LSBRⅡ、、SBRⅠ.Ca2+0～200mg/LCa2+、、、Ca2+.Schmidt4Mg2+.2.4SBR479～1114、2223.、SBR1.1SBRⅠ、ⅡTable1ElementalanalysisofseedsludgeaerobicgranulebyX-rayfluorescenceXRFNa/%Mg/%Al/%Si/%K/%Ca/%Ti/%Fe/%P/mg·L－11.042.9015.2256.421.863.780.715.6024734Ⅰ0.480.928.262.341.691.640.1610.0264257Ⅱ0.300.848.262.260.972.290.1313.42575701Ⅰ、ⅡCa2+2.14%、1.49%Fe4.42%、7.82%Al、Mg、K、Na.pH6.5～7.5pH6.9～7.2.Wang12.19EPSFeCa、Mg、K、NaEPSFe.EPSFe、Ca、MgEPS.2.5SBR、NH+4、TN、TP、COD5、6.70dSBRⅠ、ⅡNH+4、COD90%TN、TP5SBRⅠFig.5PollutantremovaleffectsofSBRⅠduringoperationperiod65%～70%SBRⅡSBRⅠ.1P2.3～2.6P.2SBR70minDO0.3～1.25mg/LDO5.0～7.5mg/L27215Ca2+6SBRⅡFig.6PollutantremovaleffectsofSBRⅡduringoperationperiodTNTP.31Ca2+30、100mg/LSBRⅠ、Ⅱ20d.50dSBRⅠMLSS、SVISBRⅡSBRⅠ、Ⅱ.Ca2+DO.2SBRCa2+ZetaZeta.Ca2+Ca2+.3Ⅰ、ⅡCa、Mg、K、NaFe.EPS.4SBRⅡSBRⅠ.70dSBRⅠ、ⅡNH+4、COD90%TN、TP65%～70%.1AdavSSLeeDJShowKYetal.AerobicgranularsludgerecentadvancesJ.BiotechnolAdv2008265411-423.2.J.2009293449-473.3.J.2007284795-799.4SchmidtJEAhringBK.GranularsludgeformationinupflowanaerobicsludgeblanketUASBreactorsJ.BiotechnolBioeng1996493229-246.5RoseRK.TheroleofcalciuminoralstreptococcalaggregationandtheimplicationsforbiofilmformationandrentationJ.BiochimBiophysActa20001475176-82.6ApostolosVElliMBSofiaM.Influenceofferro