CANON工艺处理猪场沼液的启动及微生物种群结构分析王子凌

3810201810ActaScientiaeCircumstantiaeVol．38No．10Oct．2018!No．2015-HM01-00477-SFNo．17ZA0067No．J201614SupportedbythePeople-benefitProjectofChengduScienceandTechnologyBureauNo．2015-HM01-00477-SFtheScientificＲesearchProjectofSichuanProvinceEducationDepartmentNo．17ZA0067andtheYouthAcademicLeaderＲesearchFundProjectofChengduUniversityofInformationTechnologyNo．J2016141993—[e-mail]*[e-mail]1993—[e-mail]*[e-mail]10．13671/j．hjkxxb．2018．0207．2018．CANONJ．38103945-3953WangZLXinXWangLetal．2018．Start-upandmicrobialcommunitiesanalysisofCANONprocessforthetreatmentofanaerobicdigesterliquorofswinewastewaterADLSWJ．ActaScientiaeCircumstantiae38103945-3953CANON，*，，，，6102252018-04-062018-05-082018-05-0830±1℃DO0．5±0．1mg·L－1CANONCompletelyautotrophicnitrogenremovalovernitrite．210dCANON450mg·L－169．8%61．1%NO－3-N+NO－2-N/ΔNH+40．11．CANONProteobacteria36．16%、Chloroflexi14．22%、Planctomycetes11．07%、Acidobacteria10．41%Chlorobi12．82%Candidatus_BrocadiaANAMMOX、NitrosomonasAOB、ComamonadaceaeXanthomonadaceae．CANON0253-2468201810-3945-09X703AStart-upandmicrobialcommunitiesanalysisofCANONprocessforthetreatmentofanaerobicdigesterliquorofswinewastewaterADLSWWANGZilingXINXin*WANGLuoDUＲongtingLIYilingGOUYuxiaCollegeofＲesourcesandEnvironmentChengduUniversityofInformationTechnologyChengdu610225Ｒeceived6April2018receivedinrevisedform8May2018accepted8May2018AbstractAsuccessfulstart-upofacompletelyautotrophicnitrogenremovalovernitriteCANONprocesstreatinganaerobicdigesterliquorofswinewastewaterADLSWwasachievedinacontinuous-flowbiofilmreactorCFBＲbygraduallyincreasingtheinfluentammoniumNH+4-NconcentrationandcontrollingthelowdissolveoxygenDO0．5±0．1mg·L－1．Thetemperaturewasmaintainedat30±1℃．High-throughputsequencingtechniquewasusedtomonitortheevolutionofthebacterialcommunity．Theresultsdemonstratethatasuccessfulstart-upofCANONprocesswasaccomplishedin210d．Whentheinfluentammonianitrogenconcentrationwas450mg·L－1theaverageammoniumremovalefficiencyandtotalnitrogenremovalefficiencywasupto69．8%and61．1%respectively．AndtheeffluentNO－3-N+NO－2-N/ΔNH+4-Nratiowasclosedto0．11．AtthesametimecomparedtotheseedsludgethemicrobialcommunitieshadremarkablechangesinthesludgeofCANONprocess．ThedominantmicrobialpopulationatphylumlevelwereProteobacteria36．16%Chloroflexi14．22%Planctomycetes11．07%Acidobacteria10．41%andChlorobi12．82%．ThedominantbacterialspeciesrelatedtonitrogentransformationwereCandidatus_BrocadiaNitrosomonasComamonadaceaeandXanthomonadaceae．KeywordsCANONanaerobicdigesterliquorofswinewastewaterADLSWhigh-throughputsequencingbiologicalcommunity381Introduction．、20152004．-2017．CODCOD．2013．．CANONCompletelyautotrophicnitrogenremovalovernitrite．Ammonia-oxidizingbacteriaAOB1ANAMMOX2CANON3Sliekersetal．2002．1NH+4+1．5O2→1NO－2+2H++H2O11NH+4+1．32NO－2+H+→1．02N2+0．26NO－3+2H2O21NH+4+0．85O2→0．44N2+0．11NO－3+0．14H++1．43H2O3CANON50%．2017SBＲ15%20%ANAMMOXpH=7～8、30±1℃46d35dCANON0．35kg·m－3·d－1．2014CANON30±1℃、HＲT=9h、pH=7～860dCANONTN0．79kg·m－3·d－1．Sliekers2003SBＲANAMMOX30℃、pH=7．849dCanon85%．2017SBAF30±2℃DO48dCANONNH+4-NTN99．9%86．5%．CANON、CANON．CANONCANONCANONCANON．2Materialsandmethods2．11．1．8L5∶1．30%．PVC1SBＲ1．2．3．4．5．6．7．8．Fig．1Schematicdiagramofexperimentdevice649310CANON40%8mm8mm．DO0．5±0．1mg·L－1．CANON4150、250、350、450mg·L－1．30±1℃HＲT1．5d．2．21．mg·L－1CuCl2·6H2O0．1、ZnSO4·7H2O0．1、FeCl30．3、H3BO40．1、CoCl20．1、EDTA0．1．1．1Table1KeywaterqualityoftheinfluentCODmg·L－1150～350TNmg·L－1200～500TPmg·L－120～45NH+4-Nmg·L－1150～450NO－3-Nmg·L－12．0～5．0NO－2-Nmg·L－10．5～1．0TSSmg·L－13．0～10．0pH7．5～8．02．3COD、NH+4-N、NO－3-N、NO－2-N、TN2002．DOpHJPB-607ApHTestr30pH．FAAnthonisenAnthonisenetal．1976．2．4CANON210dBlank、Ｒ_AS_SDDNADNA20160．8%DNAPCＲDNA－20℃．16SrＲNAF338ACTCCTACGGGAGGCAGＲ518ATTACCGCGGCGCTGGPCＲMuyzeretal．1993PCＲ96℃5min50℃30s72℃30s3072℃5min．2%PCＲAxyPrepDNAAXYGENPCＲYangetal．2007．PCＲQuantiFluorTM-STPromega2016PCＲIlluminaMiseq．Prinseq、、、Paired-endreadsUniqueTags97%OUToperationaltaxonomicunitsGreenGenesＲelease13．5http//greengenes．Secondgenome．com/．OTUOTU、2015．phylum、class、genus．3Ｒesultsandanalysis3．13．1．1NH+4-NTNCANONNH+4-N、TN2CANON4．Ⅰ1～40dNH+4-N150mg·L－1．NH+4-NNH+4-N93．85%．TN27．24%NH+4-NNO－3-NNO－2-NTN．Ⅱ40～90dNH+4-N250749338mg·L－1NH+4-N93．21mg·L－163．02%TN191．85mg·L－126．78%．NH+4-NTN58d56．95%19．55%60dNH+4-NTN86d67．07%35．34%．AOBANAMMOXNOBZhangetal．2013．Ⅲ90～150dNH+4-N350mg·L－1NH+4-N185．16mg·L－147．16%90～128dFA9mg·L－1．FANH+4-NFA8．5mg·L－1AOBVanetal．20072016NH+4-N．3TN118d18．02%．2016NH+4-NNO－2-NNH+4-NCANONNO－2-NNO－2-NAOB．NO－2-NCANONLottietal．2012．NO－2-NAOBNH+4-NTN126d144d62．99%35．28%．Ⅳ150～210dNH+4-N450mg·L－1NH+4-N52．2%NH+4-N215．15mg·L－1TN40．6%271．54mg·L－1．210dNH+4-NTN69．8%61．1%．2017AUSBAUSBHＲTCANON80%69%．2017450mg·L－12017．2NH+4-NaTNbFig．2ChangeofNH+4-NaandTNbremovalefficiency849310CANON3．1．2NO－3-N+NO－2-N/ΔNH+4-NCANONNH+4-NN211%NO－3-NNO－3-N+NO－2-N/ΔNH+4-NCANONDavereyetal．2012NO－3-N+NO－2-N/ΔNH+4-NCANON0．11．CANONNO－3-N+NO－2-N/ΔNH+4-N3．1～40dNO－3-N+NO－2-N/ΔNH+4-N1．0493．85%27．24%．NOB．．Ⅱ40～90dNO－3-N+NO－2-N/ΔNH+4-N．CANON200mg·L－1NOBLiuetal．2012．250m