DO对高氨氮渗滤液短程生物脱氮反硝化过程动力学的影响孙洪伟

30520105　　　　　　ActaScientiaeCircumstantiaeVol.30,No.5May,2010:(No.50978003);(No.8091001)SupportedbytheNationalNaturalScienceFoundationofChina(No.50978003)andtheBeijingNaturalScienceFoundation(No.8091001):(1976—),,,E-mail:[e-mail];＊(),E-mail:[e-mail]:SUNHongwei(1976—),male,Ph.D.,E-mail:[e-mail];＊Correspondingauthor,E-mail:[e-mail],,,.2010.DO[J].,30(5):935-940SunHW,WeiDY,WangSY,etal.2010.TheeffectofDOonthekineticsofdenitritationandpartialnitrogenremovalfromlandfillleachatewithhighammoniaconcentration[J].ActaScientiaeCircumstantiae,30(5):935-940DO孙洪伟1,魏东洋2,王淑莹1,时晓宁1,张树军1,杨庆1,彭永臻1,＊1.北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京1001242.环境保护部华南环境科学研究所,广州510655:2009-07-27　　　:2009-12-13　　　:2010-02-26:DONO-2-N,/UASB-SBR,SBR(120d),SBRpH8.0DO,DONO-2-N(K′O).,DONO-2-N,,DO0.40.6mg·L-1,49.0%16.3%.DONO-2-N.DONO-2-NK′O,,DO,K′O.:;;;;:0253-2468(2010)05-935-06　　　:X703.1　　　:ATheeffectofDOonthekineticsofdenitritationandpartialnitrogenremovalfromlandfillleachatewithhighammoniaconcentrationSUNHongwei1,WEIDongyang2,WANGShuying1,SHIXiaoning1,ZHANGShujun1,YANGQing1,PENGYongzhen1,＊　1.KeyLaboratoryofBeijingWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing1001242.SouthChinaInstituteofEnvironmentalSciences,SEPA,Guangzhou510655Received27July2009;　　　receivedinrevisedform13December2009;　　　accepted26February2010Abstract:Theeffectofdissolvedoxygen(DO)onthekineticsofdenitritationusingnitriteaselectronacceptorwasinvestigatedinthisstudy.Alab-scaleUASB-SBRsystemwasoperatedtoachievenitrogenremovalvianitritefromlandfillleachatewithhighammoniaconcentration.StablepartialnitrationwassuccessfullyachievedintheSBRafter120days.BatchtestswereperformedtoassesstheeffectofDOconcentrationontheDOinhibitioncoefficient(K′O)ofdenitritationusingthesludgefromtheSBRatdifferentDOgradientsandconstantpH8.0.DOhasasignificantinhibitoryeffectontheactivityofdenitrifyingbacteria.Thenitritereductionratedecreasedapproximately49%and16.3%ofitsmaximumrateatDOconcentrationsof0.4mg·L-1and0.6mg·L-1,respectively.AnegativeexponentialformulawasestablishedtodescribetherelationshipbetweenDOconcentrationandnitritereductionrate.DistinctvalueswereobtainedbylinearregressionwithinthreedifferentDOranges(DO≤0.4mg·L-1,0.4mg·L-1≤DO≤0.72mg·L-1and0.72mg·L-1≤DO≤1.03mg·L-1).However,theK′OwasconstantineachDOrange.Keywords:landfillleachate;denitritationkinetics;DO;inhibitioncoefficient1　(Introdcution)(DO),,O2NO-3/NO-2.,O2,NO-3/NO-2(,2006).O2:①O2(+0.82V)NO-3/NO-2(+0.42V),(Lansing,2001);②DOI:10.13671/j.hjkxxb.2010.05.016　　　　　　　　　　30,(,2006).,DO0.15mg·L-1(,2002).,DO.,,DO≥0.2mg·L-1,(Pseudomonas)(Skermanetal.,1957;Teraietal.,1975;Dawsonetal.,1972).Nelson(1978),DO0.13mg·L-1,.DO(rDN),DO(1)(MetcalfEddy,2003).rDN=k×X×SKs+SNO-xKs,NOx+NO-xK′OK′O+DO(1),rDN(g·g-1·d-1);S(mg·L-1);NO-x(mg·L-1);Ks(mg·L-1);Ks,NOxNOx(mg·L-1);K′O;X(g·L-1);DO(mg·L-1)DO(A/O,A2O).DO,.DODO()(TN).,3.①,、;②NO-3,NO-2;③.20℃,pH,,、、,,.,DO.,pH8.0(pH8.0SBRpH),DO,,,DO.2　(Materialsandmethods)2.1　试验装置及分析方法(2010)./UASB-SBR,SBR(,2010).,WTW(pH/Oxi340i,WTW)DO、pH.NO-2-NrDN,DO/rDN,rDN,rDN,DODONO-2-N(Zhouetal.,2007).2.2　试验方案SBR7L,72L.NaNO210mg·L-1NO-2-N,NO-2-N40mg·L-1.0.1mol·L-1HCl,pH8.0±0.1.(28.0±0.6)℃,VSS(1.25±0.11)g·L-1.,DO.DONO-2-N,,DO.3　(Results)3.1　DO对NO-2-N还原过程的抑制pH8.0,DONO-2-N.NO-2-N,(2):r=C0-Ctxt(2),rNO-2-N(g·g-1·h-1);CtNO-2-N(mg·L-1);t(h);x(mg·L-1).13DO(,0.60.82mg·L-1),NO-2-N.NO-2-N45mg·L-1,,DO0.6mg·L-1DO0.82mg·L-1,NO-2-N.2DONO-2-N.9365:DO1　DO,NO-2-NFig.1　NitriteconcentrationatdifferentDOconcentrations2　DOFig.2　InhibitoryeffectofDOonthenitritereductionactivityofdenitrifiers3.2　NO-2-N还原过程K′O值求解方法的建立Monod,NO-2-N(Monod,1949):rNO-2-N=kSKs+S(3)DONO-2-N(3)DO(MetcalfEddy,2003):rNO-2-N,DO=kSKs+SK′OK′O+DO(4),rNO-2-N,rNO-2-N,DODO,NO-2-N(g·g-1·d-1);kNO-2-N(g·g-1·d-1);Ks(mg·L-1);SNO-2-N(mg·L-1);K′ODONO-2-N(mg·L-1).(3)(4):rNO-2-N,DO=rNO-2-NK′OK′O+DO(5)(5)rNO-2-N:rNO-2-NrNO-2-N,DO=K′O+DOK′O=1+DOK′O=1+1K′ODO(6)DO,rNO-2-NrNO-2-N,DO,,1K′O,DOK′O.3.3　不同DO下以NO-2-N为电子受体K′O值的求解DO,rNO-2-NrNO-2-N,DODO3.3,,y=1.10×exp(x/0.36)-0.42,R2=0.983.,.(1)rNO-2-N,DODO:937　　　　　　　　　　303　rNO-2-NrNO-2-N,DODOFig.3　RelationshipbetweenrNO-2-NrNO-2-N,DOandDOconcentrationsrNO-2-N,DO=rNO-2-N1.10×expDO0.36-0.42(7),(,pH),rNO-2-N,(7)rNO-2-NrNO-2-N,DODO.,rNO-2-N0.443g·g-1·d-1,(7),:rNO-2-N=0.4431.10×expDO0.36-0.42(8)(2)DO,K′O3.2,rNO-2-NrNO-2-N,DODO.,3,,41.4　NO-2-NFig.4　SlopeoftheinhibitioncoefficientofnitritereductionwithindifferentDOconcentrationranges1　DOTable1　Inhibitioncoefficient(K′O)withindifferentDOconcentrationranges　DO/(mg·L-1)K′O/(mg·L-1)R2DO≤0.4y=0.83+2.8x0.3510.9750.4≤DO≤0.72y=-4.9+16.9x0.0590.9270.72≤DO≤1.03y=-15.8+32.0x0.0310.944　　:yrNO2-N/rNO2-N,DO;xDO,mg·L-1.4　(Discussion)4.1　DO对微生物NO-2-N还原活性的抑制分析,,DO0.6mg·L-10.82mg·L-1,NO-2-N0.443,0.0720.041g·g-1·d-1.,NO-2-NDO,,DONO-2-N,NO-2-N.2,DONO-2-N.DO,.DO0.4、0.60.82mg·L-1,49.2%、16.3%9.2%.,DO0.19mg·L-1,79%.2b,DO0.6mg·L-10.62mg·L-1DO1.03mg·L-1,DO,,0.9.4.2　DO对以NO-2-N为电子受体K′O值的影响分析4,DO,DOK′O,,0.9.DO,K′O.,DOK′O.DO,K′O,DO,K′O,K′ODONO-2-N.DO,K′ONO-2-NDO.DO,K′ONO-2-NDO.Ba