DR2500方法说明书

59000-22ODYSSEYDR/2500分光光度计分析操作手册哈希公司（HACH）2003目录Atrazine···························5钡浊度法···························7苯并三唑或甲苯并三唑·····················8硼胭脂红法··························10硼甲亚胺-H法·························11溴·······························14氯胺（Mono）靛酚法HR····················16氯胺（Mono）靛酚法LR····················17氯化物硫氰酸汞法·······················18二氧化氯直读HR·······················19二氧化氯DPD·························21二氧化氯氯酚红法LR·····················22余氯······························23余氯DPDMethod····················24总氯DPDMethod·······················26总余氯DPDMethodTNT····················27余氯DPDMethodTNT····················30总氯DPDMethod·······················31六价铬····························32总铬·····························33钴PAN法··························36真色度和表观色度·······················38铜·······························39铜叶啉法··························42氰化物·····························44氰尿酸·····························46氟·······························47甲醛······························48硬度钙和镁··························52总硬度ULR··························54联氨······························55碘······························56铁FerroZine法························58亚铁1,10-菲绕啉法······················60总铁FerroVer法··························61总铁TPTZ法···························62总铁FerroMo法··························64铅·································65锰HR·······························68锰LR·······························69汞·································712钼HR·······························75钼LR·······························77镍·································79镍Heptoxime法··························81硝酸根离子·····························83硝酸盐TNT····························86亚硝酸盐······························87亚硝酸盐HR····························90亚硝酸盐LRTNT··························91氨氮HR·····························92氨氮LR·····························95总氮LR·····························96总氮HR·····························98总无机氮·····························100总凯氏氮·····························102总有机碳HR···························105总有机碳LR···························106COD锰法·····························108COD铬法·····························111溶解氧LR·····························115溶解氧UHR····························116氧SCAVENGERS··························117臭氧································119苯酚································120磷酸盐UV法····························122磷TNT······························125活性磷氨基酸法··························127活性磷·······························129活性磷抗坏血酸法·························131活性磷TNT法··························132总磷································133总磷TNT·····························134钾···································136季铵盐·································137硒···································138硅···································141硅HR·································143银···································145硫酸盐·································147硫化物·································148阴离子洗洚剂······························150悬浮固体································152丹宁及木质素硫酸盐······························153毒性··································1553TPH··································157TPH土壤中···························160挥发性酸································165锌···································1674Atrazine免疫法方法号：10050水中免疫程序1.按SingleWavelength，按，按450nm，按OK。2.标记每一抗体试管，为校准管和样品准备。3.将试管放入架中。4.吸取0.5ml校准液到样品管中。5.吸取0.5ml样品液到样品管中。6.立刻吸取0.5mlAlachlorEnzymeConjugate液到每一样品管中。7.按timeicon,按OK，开始20分钟反应。使用MicroCuvetteRack技术，先快迅摇晃试管30秒。8.十分钟后，再摇晃30秒。9.反应后，倒去试管中液体。10.用去离子水，清洗4次，保证清洗干净，并将水倒干。5显色11.保持试管在架中，加入0.5ml显色液。12.按timeicon,按OK，开始10分钟反应。13.五分钟后，再摇晃30秒。注：部份或全部溶液显蓝色。14.反应后，按加显色液顺序加入停止显色液，并放入架中，摇晃。注：蓝色溶液变黄色。15.在标记为0管中加入去离子水，并擦干净。16.放入1cm圆管适配器。17.按ZERO，显示0.000Abs。18.放入第一个校准液，按READ，显示一吸光值，记下。19.重复18步，测出每一校准液和样品的值。结果计算如果样品读数样品Alachlor浓度小于校准液读数大于该校准液浓度大于校准液读数小于该校准液浓度6钡浊度法(药剂法和安培瓶法)1~100mg/L（方法号：8014）1.在HACHPROGRAM下，选择钡法程序编号20，按START。2.往样品管中装入25mL样品。3.加入一包BariVer4Barium试剂粉包到样品管（待测试样）。旋转混合。注：如存在钡将出现白色浑浊。4、按TIMERICON，按OK。开始5分钟的反应计时。注：在生成浑浊的5分钟期间，不要搅动样品。5.往另一个样品管中加入25mL样品（空白试样）。6.当计时器鸣叫时，将空白试样放入样品管槽。关上遮光盖。7.按ZERO，屏幕显示：0.0mg/LBa。注：如使用试剂空白校正，屏幕将显示校正值。8.计时器鸣叫后10分钟内，将预制试样放入样品管槽，盖上遮光盖，按READ。屏幕将显示钡的含量，单位是mg/L。以下物质超过表中所列的浓度时会引起干扰。干扰物质干扰水平和处理钙10,000mg/L的CaCO3镁100,000mg/L的CaCO3硅500mg/L氯化钠130,000mg/L的NaCl高缓冲液样品及极端PH需要样品前处理锶所有水平上干扰。如果存在锶，钡和锶的总浓度可用沉淀物表示（硫酸盐的沉淀物）。当这样不能区分钡和锶的情况下，要进一步分析两者比例。7苯并三唑或甲苯并三唑（1-16mg/L或1-20mg/L）Method8079UVPhotolysisMethod*1.在HACHPROGRAM下，选择苯并三唑法程序编号30，甲苯并三唑法程序编号730，按start。2.往样品管中装入25mL样品。3.加入一包三唑试剂粉包。4.旋转混合使完全溶解。5.戴上紫外保护镜6.在样品管中嵌入紫外灯。7.打开紫外灯。按TIMERICON，按OK，并开始5分钟的反应计时。注：如有三唑存在，将呈黄色。8.当计时器鸣叫时，关紫外灯。从样品管中取出紫外灯。旋转样品管使充分混合。9.往另一个比色瓶中装入25mL样品（空白试样）。10.将空白试样放入样品管槽，关上遮光盖。11.按ZERO。屏幕显示：0.0mg/LBenzo或0.0mg/LToly。12.将预制试样放入样品管槽，关上遮光盖，按READ。屏幕将显示苯并三唑或甲苯并三唑的含量，单位是mg/L。8干扰表1干扰物质和建议的处理方法干扰物质干扰水平和处理丙烯酸盐(甲基丙烯酸盐)大于50mg/L。明矾大于400mg/L。硼酸盐(四硼酸钠)大于4000mg/L。氯(Cl2)大于20mg/L。铬(铬酸盐)大于12mg/L。铜大于10mg/L。硬度大于500mg/L的CaCO3。铁大于20mg/L。Lignosulfonates大于40mg/L。镁大于300mg/L的CaCO3。钼(钼酸盐)大于200mg/L。亚硝酸盐大于4000mg/L。Phosphonates(AMPorHEDP)大于100mg/L。硫酸盐大于200mg/L。锌大