EGSB反应器在20下处理啤酒废水的工艺及微生物学研究李津

EGSB20℃,＊,(,　100084):20℃(EGSB),、,:①HRT18h,184d,EGSB(COD)10kg(m3·d),COD85%,1kgCOD0.58m3;②,,,10kg(m3·d),;,(ECP),;③,20℃,15℃6.:EGSB;;:X797　:A　:0250-3301(2008)04-0990-06:2007-04-24;:2007-07-23:(50678091):(1982～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]lCharacteristicsofanEGSBReactorTreatingBreweryWastewaterUnder20℃LIJin,ZUOJian-e,XINGWei(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China)Abstract:Alab-scaleEGSBreactorfedbyartificialbrewerywastewaterwasoperatedunder20℃continuously,anditsprocessperformance,characteristicsofgranularsludge,microbialcommunitystructurewereinvestigated.Theresultsindicatedthat:①after184days'operation,theorganicloadingrateofEGSBreactorcouldreach10kg(m3·d)atHRTofabout18h,theCODremovalefficiencieswereabove85%,andthespecificbiogasproductionwasabout0.58m3perkilogramCODremoved.②Astheorganicloadingrateincreased,theaveragediameterofthegranuleincreased,andthesettlingvelocityincreasedtoo,butwhentheloadingratereachedabout10kg(m3·d),thegranularaveragediameterdecreasedslightly.Comparedwiththegranularsludgeoperatedatmesophiliccondition,theECPcontentsespeciallytheproteincontentsinthegranularsludgeoperatedat20℃increasedobviously.③Comparedwiththeseedsludge,themainArchaea(methanogenes)speciesinthesludgesamplesoperatedatdifferentloadingratesunder20℃didnotchangegreatly,butthedominantmethanogenesinthesludgeoperatedunder15℃for6monthsseededwiththesamemesophilicsludgechangedobviously.Keywords:EGSBreactor;ECPs;microbialcommunitystructure　　,[1].,(35℃)(55℃),[2,3].,,[4～7].,,[8～11].,EGSB620℃,(extracellularpolymers,ECP),,(20℃).1　1.1　1,(expandedgranularsludgebed,EGSB),85mm,2040mm,1760mm;15L,10L,5L.,DC-101020℃.29420084　　　　　　ENVIRONMENTALSCIENCEVol.29,No.4Apr.,2008DOI:10.13227/j.hjkx.2008.04.005,,.,.1　EGSBFig.1　SchematicdiagramofEGSBreactorandprocessflowchart1.2　,,,1200mgL,7500mgL,1mL(Fe=0.13gL,Co=0.005gL,Mo=0.03gL,Ni=0.002gL)0.2mL,,pH7.0.,20Ld,HRT18h,.1.3　(35℃)UASB,0.6～2.0mm,VSSSS0.86,26.7gL.1.4　COD:TL-1ACOD;pH:Orion828;:;:LML-2;:[3];:[12];:S-570;SSVSS:[3];:1m[3];:,TP-2060T,2m(3×0.5mm),50℃;(ECP):EDTA,,[13～16].PCR-DGGE:DNA,PCRPTC-200(MJ),16SrDNAV2-V3,PARC109FPRUN518R,DGGEDcode(Bio-Rad),8%,30%～55%,60℃10～12h,SYBRGold.2　2.1　20℃EGSB,1.5kg(m3·d),184d,50d.EGSBCOD、2,3.20d,20℃.,EGSBCOD1200mgL,COD50%～70%,,,23.COD85%,.,EGSB51.6kg(m3·d)2.6、4.0、6.610kg(m3·d),2,EGSB3～5d,COD,3～5d,COD85%,6.6kg(m3·d)10kg(m3·d),10dCOD70%,6d,COD85%.,,COD,72、8096d,,,18℃,COD74%80%69%,;,,143、172d,18℃,COD,,.3,EGSB(),,9914:EGSB20℃0.3L(L·d)4.8L(L·d).,,EGSB20℃1kgCOD0.58m3.75%,,,:CO2,,.2　EGSB20℃Fig.2　PerformanceofEGSBreactorat20℃3　20℃EGSBFig.3　RemovalorganicloadingratesandspecificbiogasproductionratesofEGSBreactorat20℃2.2　6,,4.,1.4mm56%85%,2mm,30%70%.10kg(m3·d),,,1.4mm,2mm10,10kg(m3·d)、,,.2mm1.4～2mm,;,,:,Allen[17],5.1.05gcm3,　　　　　　4　Fig.4　GranulardiameterdistributionatdifferentOLRs5　Fig.5　AveragegranulardensitiesatdifferentOLRs992　　　　　　291.0～1.03gcm3,;10kg(m3·d).,,,,10kg(m3·d).,,,.,,,.20℃,,,,,.184d,,,6.,,,[6(a)];,[6(b)];[(6(c)];,12,,[6(d)];,70～80μm,,,[6(e)];[6(f)],.6　20℃184dFig.6　SEMphotographsofgranulesfromEGSBreactorat20℃after184days'operation2.3　ECPECP[13～16].7,EGSB.:①,ECP.4.0、6.610.0kg(m3·d),(VSS):11、13、14mgg17、27、56mgg;②ECP;③1.5∶1～4∶1,ECPVSS2.8%～7.0%.UASB,35℃,10kg(m3·d),,14mgg,44mgg.20℃EGSB,35℃ECP,.,ECP,.ECP,9934:EGSB20℃,.7　20℃ECPFig.7　ECPcontentsandcompositioninthesludgeoperatedat20℃2.4　[18～21],[22～24],[24,25].PCR-DGGE、20℃EGSB15℃6(),8.DGGE,,.8A15℃,B,C、D、E20℃.8:①,,;②320℃,,15℃6,,:15℃6(A)81、2,(B)320℃(C、D、E).,B、C、D、E,A,A.3　(1),20℃A:15℃;B:(35℃);C:20℃,1.6kg·(m3·d)-1;D:20℃,2.6kg·(m3·d)-1;E:20℃,4.0kg·(m3·d)-18　DGGEFig.8　DggefingerprintofthesamekindofsludgeunderdifferenttemperaturesEGSB,HRT18h,184d,10kg(m3·d),COD85%,1kgCOD0.58m3.(2),,.10kg(m3·d),,.ECP,,ECP,,.(3),20℃,,15℃6.:[1]LettingaG,RebacS,ZeemanG.Challengeofpsychrophilicanaerobicwastewatertreatment[J].TrendsinBiotechnology,2001,19(9):363-370.[2],.[M].:,2004.29.[3].[M].:,1998.522-560.[4]LettingaG,VanlierS.Advancedanaerobictreatmentinnearfuture[J].WaterScienceTechnology,1997,35(10):5-12.[5].[J].,1998,19(1):94-96.[6]LettingaG,RebacS,ParshinaS,etal.High-rateanaerobictreatmentofwastewateratlowtemperature[J].AppliedEnvironmentalMicrobiology,1999,65(4):1696-1702.[7]RebacS,RuskovaJ,GerbensS,etal.High-rateanaerobictreatmentofwastewaterunderpsychrophilicconditions[J].FermentandBiotechnol,1995,80(5):499-506.[8]NozhevnikovaAN,SimankovaMV,ParshinaSN,etal.Temperaturecharacteristicsofmethanogenicarchaeaandacetogenicbacteriaisolatedfromcoldenvironments[J].WaterScience994　　　　　　29Technology,2001,44(8):41-48.[9]SharonMH,CartonM.Methanogenicpopulationstructureinavarietyofanaerobic[J].FEMSMicrobiologyLetters,2003,219:297-304.[10]ConnellyTL,TilburgCM,YagerPL.EvidenceforpsychrophilesoutnumberingpsychrotolerantmarinebacteriainthespringtimecoastalArctic[J].LimnologyandOceanography,2006,51(2):1205-1210.[11],.[J].,1999,26(2):155.[12],.[P].():200510011874.8,2005-11-16.[13],,,.、[J].,1994,15(4):23-26.[14]SponzaDT.Extracellularpolymersubstanc