EGSB反应器中耦合厌氧氨氧化与甲烷化反硝化的研究祖波

EGSB祖波1,张代钧1,2*,白玉华1,张萍11.,4000302.,400030:(EGSB),120d,.EGSB,32~35e,pH715~813,-150~-40mV;412h,4186mPh,270d,.Q(CODCr)500mgPL,41800kgP(m3#d)(CODCr)11152kgP(m3#d)(N),Q(CODCr)80mgPL;CODCr,,85%,35%,9919%67%;6112,01202,0157501777kgP(m3#d);P(0105kgP(m3#d))41515.pH,,,,CODCrEGSB.:;;;:X703:A:1001-6929(2007)02-0051-07StudyofIntegrationofMethanogensiswithSimultaneousAnaerobicAmmoniumOxidationandDenitrificationinEGSBReactorZUBo1,ZHANGDai-jun1,2,BAIYu-hua1,ZHANGPing11.DepartmentofEnvironmentalSciences,ChongqingUniversity,Chongqing400030,China2.KeyLaboratoryofSouthwest-ChinaResourceExploitation&EnvironmentalDisasterControlEngineering,MinistryofEducation,ChongqingUniversity,Chongqing400030,China:2006-07-05:(50378094);(200609Y1A0260185):(1980-),,,.*Abstract:GranularsludgewasformedinEGSBreactorinoculatedwiththeaerobicactivatedsludgeafterthereactorstart-upoperationof120d.Then,denitrificationbacteriaandanaerobicammoniumoxidationbacteriawereculturedandenrichedfor270dinEGSBreactorwithnitriteandammonium.ThepHwascontrolledbetween715and813,redoxpotentialat-150~-40mVandtemperatureat32~35einthereactor;HRTwas412h,andtheup-velocitywas4186mPh.UndertheconditionsthatCODCrconcentrationwasabout500mgPLandthevolumetricloadingrateabout41800kgP(m3#d)(estimatedbyCODCr)and11152kgP(m3#d)(estimatedbyN)intheinfluent,CODCrconcentrationwasstable,below80mgPLintheeffluent,theremovalefficiencyofCODCr,ammoniumnitrogen,nitritenitrogenandtotalnitrogenwas85%,35%,9919%and67%,respectively;theremovalrateofCODCr,ammoniumnitrogen,nitritenitrogenandtotalnitrogenwasstable,about6112,01202,01575and01777kgP(m3#d),respectively;theremovalrateofammoniumnitrogenandtotalnitrogenis4and1515timeshigherthanthatinaconventionalactivatedsludgesystemwithnitrificationPdenitrification(0105kgP(m3#d)),respectively.ResultsdemonstratedthatpH,temperature,DO,redoxpotential,nitrite,CODCraffectedtheintegrationofmethanogenesisbacteria,denitrificationbacteriaandanaerobicammoniumoxidationbacteriaandthecharacteristicofgranularsludgeinEGSBreactor.Keywords:anaerobicammoniumoxidation;methanogensis;denitrification;integration202ResearchofEnvironmentalSciencesVol.20,No.2,2007DOI10.13198/j.res.2007.02.53.zub.0112070Lettinga(upflowanaerobicsludgebed,UASB),[1].90,(extendedgranularsludgebed,EGSB)(3~8e)(Q(CODCr)200mgPL)CODCr90%[2].,,,,CODCr,[1].,,.,,.P,,.P,2:¹,;º,[3].,[4)5].EGSB,;,EGSB.11.1EGSB1.,215L,115L,61mm,13.3,,,EGSB32~35e.NaOH,.1.2EGSB:(164mgPL),(NH4)2SO4(716mgPL),KH2PO4(3104mgPL),K2HPO4(3196mgPL),NH4Cl(2014mgPL),NaHCO3(2000mgPL),A(210mLPL).A:EDTA(510gPL),FeSO4#7H2O(510gPL),ZnSO4#7H2O(212gPL),CoCl2#1);2);3);4);5);6);7);8);9);10)1EGSBFig.1SchematicdiagramofEGSBreactor6H2O(116gPL),CaCl2#2H2O(515gPL),MnCl2#4H2O(510gPL),CuSO4#5H2O(116gPL),MgSO4#7H2O(510gPL),(NH4)6Mo7O24#4H2O(111gPL),NiSO4#6H2O(0142gPL).:(13mL),(NH4)2SO4(716mgPL),KH2PO4(3104mgPL),K2HPO4(3196mgPL),NaHCO3(1400mgPL),NH4Cl(14816mgPL),NaNO2(18715mgPL),A(210mLPL).1.3EGSB:2005227)627.EGSB22g,Q(CODCr)180mgPL,10mLPmin,180mLPmin,(CODCr,)11728kgP(m3#d),(CODCr,)0119kgP(kg#d),412h,4186mPh.:EGSBNaNO2NH4Cl,.2005627)2006225,EGSBQ(CODCr)500mgPL,4180011152(N)kgP(m3#d),52200155001088(N)kgP(kg#d).EGSB.N2,pH,.1.4N-(1)-NO2--N,CODCr,MLSS,pHpH,,,NH4+-N,,1000mL[6].22.1EGSBEGSB22g,0~3d,CODCr,CODCr,,,,80mgPL,.6~30d,0126mPh0177mPh,,,(0102~0150mm),,.30~60d,0177mPh2130mPh,,,CODCr42%,,,.60~75d,2130mPh4186mPh,75d4186mPh,CODCr76%,;1~2mm,,.100~120d,CODCr81%,2~3mm,1~2mm,,,,,(2).2.2EGSB,NaNO2NH4Cl,.2.2.1CODCrEGSBQ(CODCr)400mgPL2EGSBFig.2Thestart-upofEGSBreactor,500mgPL,CODCr71%85%;80mgPL(3).3,NaNO2NH4Cl,CODCr,CODCr.CODCr,.,CH4N2.3CODCrFig.3TheCODCrremovalintheintegrationstage2.2.24,EGSBQ()30mgPL40mgPL.3,,,,,.,,.46d,;,.2.2.33(0~46d),Q(),532:EGSB4Fig.4Thenitritenitrogenremovalintheintegrationstage.,Q().,.46d,Q().,,;200d,EGSB35%,(5)..Q(),pH,NH3.5Fig.5Theammoniumnitrogenremovalintheintegrationstage2.2.4EGSB,6.6,,200d.EGSB67%,.2.2.5EGSB6Fig.6Thetotalnitrogenremovalintheintegrationstage,,,,2~3mm,1~2mm;.EGSB15~80mPh,80%30~55mPh,.33.1Q(CODCr)EGSBEGSB,210gPLNaHCO3,pH,,pH618~712,32~35e,-340mV,.EGSB.Q(CODCr)180mgPL,11728kgP(m3#d),0119kgP(kg#d),EGSB,,CODCr81%.,Q(CODCr),EGSB.Ghangrekar[7],UASB,210~415kgP(m3#d)011~0125kgP(kg#d),CODCr.EGSB,,3:,,.,4186mPh,,;805420mgPL,,.Show[8]6UASB,,80mgPL,43%,,40gP(L#d),.Alphenaar[9]UASB.[10]EGSB,EGSB,,115~415mPh.3.23.2.1pH,DO,EGSB114gPLNaHCO3,pH618~715,pH715~813,pH810~816;2000~5000mgPL.32~35e.,pH615~718,618~715;15~43e,32~35e[11].pH615~715,35~45e[12].BrocadiaanammoxidanspH617~813,20~43e;810,40e[13].KueneniastuttgartiensispH615~910,810;37e,45e,37e,11e37e24%[13].pH,,3.[11],,Q(DO)015mgPL[12].,O22LmolPL,O2[13].N2,.3.2.2,NO2-NH4+,,,-150~-40mV,-150~-50mV.pH7,NO3-PNO2-,NO2-PNO,NOPN2O,N2OPN2432,638,1173,1355mV,CO2PCH4-224mV[14].-330mV[11],NO2-PN2,.,3.,-330mV[11];,.,,.3,,,;,,,,,CODCr85%.Akunna[15](-290mV),.Roy[16](,H2,),,H2.,,[14].Belay[17]NO3-N,.Fischer[18],MethamosarcinabarkeriN2O.Clarens[19],0118mmolPLNO2-018%NOx,7114mmolPLNO3-8313%,MethanosarcinamazeiPseudomonasstutzeri,().Kluber[14],,NO,N2O(MethanosarcinabarkeriMethanobacteriumbryantii),2,N2OMethanosarcin