Fhhh工程菌株降解PTA废水动力学研究

FhhhPTA,,,,,,(,　210093):Fhhh(PTA).Fhhh6、、,(Ebis).,3Fhhh,(Vmin)1309m3,(qA)0.0136h-1,YZ14,4,2.,FhhhPTA.:;;PTA;;:X74　:A　:0250-3301(2003)06-05-0116:863(2001AA214191);(BK-99033):(1947～),,,;.:2002-11-18;:2003-01-09DegradationKineticsforFhhhStraininPTAWastewaterChengShupei,ZhangXuxiang,ShiLei,QuMengmeng,ZhouTao,HaoChunbo,YanJun(NationalKeyLaboratoryofPCRR,TheSchoolofEnvironment,NanjingUniversity,Nanjing210093,China)Abstract:Degradationkineticsofthepurifiedterephthalicacid(PTA)petrochemicalwastewaterwiththefunctionalstrainFhhhwasconductedinthisresearch.Inputtingthevaluesofthe6kineticsparametersobtainedfromthetest,thevaluesofthenaturalparameterandthevaluesofthestandardparametersfordischargingwastewaterintotheenviron-mentalbiotechnologicalinformaticssoftware(Ebis),theminimumreactorvolume(Vmin)requiredandthespecificdegradationrate(qA)werecalculatedbasedontheactivatedsludgeprocess.TheminimumvalueofVminwas1309m3aswellasthehighestvalueofqAwas0.0136h-1amongtheFhhhpreparationskeptinthreedifferentmethods.Thehigh-estvalueofFhhhqAwas4foldofthatofthenativebacteriumYZ1,andhigherthanthatofthedatapublishedby4re-searchersandlowerthanthatofby2researchers.TheresultsshowthatFhhhhastheobviouspotentialforthedegrada-tionofPTAwastewater.Keywords:functionalstrain;protoplastfusion;PTAwastewater;kinetics;informaticssoftware　　(purifiedterephthalicacid,PTA),、、.WebofScience,5PTA343[1,2].PTA,,PTA,[3,4].PTA、、3,Fhhh3.Fhhh23,.3:1,PC(Phane-rochaetechrysosporium);2,SC(Saccharomycescerevisiae);3,YZ1.FhhhPCmnp、SCFLO1YZ116SrDNA3DNA,.FhhhPC、SCYZ1[5,6].qAVmin2,FhhhPTA.(EnvironmentalBiotechnologicalInfor-maticsSoftware,Ebis)246200311　　　　　　ENVIRONMENTALSCIENCEVol.24,No.6Nov.,2003DOI:10.13227/j.hjkx.2003.06.021[13].Ebis,;,;Vstudio.NETMicrosoftVisualBasic,,.Ebis,、,,.Ebis.(Vmin).,(Vmin),.(qA),,,[7,8].qA,.qAVmin2,.,FhhhPTAqA0.0136h-1,Fhhh0.0136.SCPTA,qA.SCFLO1Fhhh,Fhhh.FhhhqAYZ14,4、2,.1　(1)　Fhhh,32,3、、.1:PC()Prof.Wang;2:SC();3:YZ1()NJYZ-PTA.(2)　1000mL:5g,20g,200g,pH=7.(3)PTA　NJYZ-PTA.COD《》;TN、TP[9,10].CODCr=4344mg/L;BOD5=2828mg/L;pH=4.9.(4)Fhhh3　3:pH=7.0、DO=0.26mg/L、X=4.99mg/L、4℃、60d.31.　　(5)1　Fhhh3Table1　FhhhstrainsfromthethreepreservationmethodsFhhh3(1)Fhhh+SH()(2)Fhhh+Fe3+()(3)Fhhh+Fe3++SHSH/%　0.020.02Fe3+/%0.140.14TN/mg·L-1305TP/mg·L-161　[11,12]:μ=(lnXn-lnX0)/t1/μ=(Ksμ/μmax)×(1/S0)+1/μmaxq=μ(S0-Se)/[X0(eμt-1)]1/q=(Ksq/qmax)×(1/S0)+1/qmax1/θc=YTq-Kd,μ(h-1);X0、Xn(mg/L);t(h);μmax(h-1);Ksμμmax/2CODCr(mg/L);qmax(h-1);Ksqqmax/2CODCr(mg/L);S0、SeCODCr(mg/L);YT1176　　　　　　(%);Kd(h-1);θc(h).3Fhhh5.,,2.,.:μ=/(·);:q=/(·),μq.,.μq.2　FhhhPTATable2　DesignationofkineticstestsforFhhhinPTAwastewaterFhhh(1)Fhhh+SH()(2)Fhhh+Fe3+()(3)Fhhh+Fe3++SH　1　2　3　4　5　1　2　3　4　5　1　2　3　4　5X0/mg·L-1725725725725725804804804804804789789789789789S0/mg·L-1434421721086543272434421721086543272434421721086543272/h666666666666666/℃353535353535353535353535353535/r·min-11201201201201201201201201201201201201201201202　　　3X0、Xn、S0Se,3FhhhPTA6.6,.3,Fhhh,Ebis[13].3　FhhhPTATable3　ThevaluesofkineticsparametersforFhhhinPTAwastewaterFhhh(1)Fhhh+SH(2)Fhhh+Fe3+(3)Fhhh+Fe3++SHμmax/h-1　0.1735　0.1113　0.0417Ksμ/mg·L-1576395486qmax/h-10.38450.61520.7296Ksq/mg·L-15635281054YT/%44.4522.2312.16Kd/h-15×10-52×10-45×10-5　　Ebis:6,;,;,.Ebis,,;,;,.,Ebis.4,,PTA10000m3,CODCr4344mg/L.4SeXe[GB8978-1996],Ebis.EbisSeXe,,、Fhhh、、.E-bis,FhhhPTA.,FhhhPTA.118　　　　　　244　EbisFhhhPTAqAVminTable4　ForecastingvaluesofqA&VminforFhhhinPTAwastewaterfromEbisFhhh(1)Fhhh+SH()(2)Fhhh+Fe3+()(3)Fhhh+Fe3++SHCODCr,Se/mg·L-1101020,Xe/mg·L-11251FhhhqA/h-10.00670.01140.0136FhhhμA/h-10.00290.00310.0017FhhhVmin/m3153514681309　　4,3FhhhPTA,qAVmin,qA,.qA,,,.43FhhhqA:0.0067h-1、0.0114h-1、0.0136h-1,3.qmax/qA(3、4)58、54、54,qmaxqA58～54.　　FhhhPTAqA5.5,PCqAFhhh,0.0914h-1.PCFhhh,PC,,.5　FhhhqA[14～19]Table5　ComparisonofqAvaluesbetweenFhhhanddatareportedq/h-1qA/h-1[14]0.0089～0.0075Fhhh+Fe3++SH,(3)0.0136[15]0.0125Fhhh+Fe3+,(2)0.0114[16]0.0583～0.0304Fhhh+SH,(1)0.0067[17]0.0121/0.00381PC0.0914[18]0.0458～0.01672SC0.0000[19]0.00833YZ10.0035　　5,(3)FhhhqA0.0136h-1,2.(3)2Fhhh.FhhhqA0.0136h-1YZ12.FhhhPCmnp.YZ1PTAA/O(anaerobic/oxygena-tion),qAA/O[14].(3),FhhhqA,[16,18],[15～17],FhhhqA.,.,.Ebis,[13].Fhhh,FhhhPTA.3　FhhhPTAqA1196　　　　　　0.0136h-1.FhhhqAYZ14.FhhhqA,(3),Fhhh.致谢:ProfessorWangPC;NJYZYZ1PTA.:1　DyatkinaNB,RobertsCD,KeicherJD,DaiYQ,Nadh-ernyJP.MinorgrooveDNAbindersasantimicrobialagents[J].JournalofMedicinalChemistry,2002,45(4):805～817.2　MacarieH.Overviewoftheapplicationofanaerobictreat-menttochemicalandpetrochemicalwastewaters[J].WaterScienceandTechnology,2000,42(5～6):201～213.3　KleerebezemR,LettingaG.High-rateanaerobictreatmentofpurifiedterephthalicacidwastewater[J].WaterScienceandTechnology,2000,42(5～6):259～268.4　LeeDK,ChangTS,ShinCH.Thermaltreatmentofcrudeterephthalicacidrecoveredfromalkaliweight-reductionwastewater[J].JournalofIndustrialandEngineeringChem-istry,2002,8(5):405～409.5　,,,,.FhhhPTA4[J].,2001,37(11):701～705.6　,,.[J].,2000,36(3):312～316.7　,KuoCH.[J].,2000,13(2):21～24.8　,,.[J].,2000,20(1):27～30.9　APHA,AWWA,WPCF.Standardmethodsfortheexami-nationofwaterandwastewater,18thed